Differential Expression of Glutathione s-Transferase Enzyme in Different Life Stages of Various Insecticide-Resistant Strains of Anopheles Stephensi: A Malaria Vector

Background & objectives: Interest in insect glutathione s-transferases (GSTs) has primarily focused on their role in insecticide resistance. These play an important role in biotransformation and detoxification of many different xenobiotic and endogenous substances including insecticides. The GST activity among 10 laboratory selected insecticide resistant and susceptible/control strains of Anopheles stephensi was compared using the substrates 1-chloro-2,4-dinitrobenzene (CDNB). The difference in the GST activities of different life stages of diverse insecticide resistant strains was compared and presented.Methods: About 100 larvae, pupae, adult males, adult females and eggs (100 μg in total weight) were collected and used for the experiment. The extracts were prepared from each of the insecticide-resistant strains and control. Protein contents of the enzyme homogenate and GST activities were determined.Results: Deltamethrin and cyfluthrin-resistant strains of An. stephensi showed significantly higher GST activity. Larvae and pupae of DDT-resistant strain showed peak GST activity followed by the propoxur-resistant strain. On contrary, the GST activity was found in reduced quantity in alphamethrin, bifenthrin, carbofuran and chloropyrifos resistant strains. Adults of either sexes showed higher GST activity in mosquito strain resistant to organophosphate group of insecticides namely, temephos and chloropyrifos. Interpretation & conclusion: The GST activity was closely associated with almost all of the insecticides used in the study, strengthening the fact that one of the mechanisms associated with resistance includes an increase of GST activity. This comparative data on GST activity in An. stephensi can be useful database to identify possible underlying mechanisms governing insecticide-resistance by GSTs

Insecticide susceptibility status in three medically important species of mosquitoes, Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus, from Bruhat Bengaluru Mahanagara Palike, Karnataka, India

Background: Development of resistance among mosquito vectors of diseases is of growing concern in many countries of the subtropics, including much of India. The distributions of susceptibility/resistance to insecticides are scarcely recorded and are unknown in many parts of India. This manuscript reports the susceptibility status among strains of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus collected from different geographical areas of Bruhat Bengaluru Mahanagara Palike (BBMP), Karnataka, to six conventionally used insecticides. Results: An. stephensi, Ae. aegypti and Cx. quinquefasciatus from different geographic areas showed susceptibility towards temephos. However, susceptibility was meagre for most of the other insecticides used in the study. Strong resistance to propoxur was reported in Ullala (RR90 = 467.1) and Bannerghatta (RR90 = 12 265) populations of An. stephensi and Ae. aegypti respectively. RR90 â 12 830 was recorded as higher in the Yelahanka (YLK) population of Cx. quinquefasciatus to neem. Significant differences at LC50 and LC90 concentrations were observed against various insecticides in different mosquito populations. Resistance was observed against some of the larvicides for the diagnostic dose recommended by WHO. Conclusion: The results form baseline data on the susceptibility status of three medically important mosquito vectors of diseases to commonly used insecticides in the field at present emphasise the importance of carrying out more effective and planned vector control measures. © 2012 Society of Chemical Industry

Symmetry Energy in the Equation of State of Asymmetric Nuclear Matte

The symmetry energy is an important quantity in the equation of state of isospin asymmetric nuclear matter. This currently unknown quantity is key to understanding the structure of systems as diverse as the neutron-rich nuclei and neutron stars. At TAMU, we have carried out studies, aimed at understanding the symmetry energy, in a variety of reactions such as, the multifragmentation of $^{40}$Ar, $^{40}$Ca + $^{58}$Fe, $^{58}$Ni and $^{58}$Ni, $^{58}$Fe + $^{58}$Ni, $^{58}$Fe reactions at 25 - 53 AMeV, and deep-inelastic reactions of $^{86}$Kr + $^{124,112}$Sn, $^{64,58}$Ni (25 AMeV), $^{64}$Ni + $^{64,58}$Ni, $^{112,124}$Sn, $^{232}$Th, $^{208}$Pb (25 AMeV) and $^{136}$Xe + $^{64,58}$Ni, $^{112,124}$Sn, $^{232}$Th, $^{197}$Au (20 AMeV). Here we present an overview of some of the results obtained from these studies. The results are analyzed within the framework of statistical and dynamical models, and have important implications for future experiments using beams of neutron-rich nuclei.Comment: 10 pages, 4 figures, talk presented at VI Latin American Symposium on Nuclear Physics and Application

Effective nucleon mass and the nuclear caloric curve

Assuming a schematic form of the nucleon effective mass as a function of nuclear excitation energy and mass, we provide a simple explanation for understanding the experimentally observed mass dependence of the nuclear caloric curve. It is observed that the excitation energy at which the caloric curve enters into a plateau region, could be sensitive to the nuclear mass evolution of the effective nucleon mass.Comment: 5 pages, 5 figures, Accepted for publication in Phys. Rev. C. Minor changes mad

Glutathione S-Transferase activity and total thiol status in chronic alcohol abusers before and 30 days after alcohol abstinence

Background: Glutathione S Transferase (GST) has been involved in detoxification process in the liver and its activity has been shown to be increased in alcohol abusers. In the current work we measured the GST activity, total thiol status, AST, ALT, and direct bilirubin in chronic alcohol abusers before and 30 days after alcohol abstinence and lifestyle modification. Methods: Serum and urine GST activity and total thiol status were determined using spectrophotometric methods and serum transaminases were determined using clinical chemistry analyzer. Results: We found,significant increase in serum and urine GST (p<0.001), AST (p<0.001), ALT (p<0.001), and decrease in total thiol status (p<0.001) in chronic alcohol abusers. GST activity significantly decreased (p<0.001) and total thiol status were improved significantly (p<0.001) 30 days after alcohol abstinence and lifestyle modification. Conclusion: This study provides preliminary data to suggest the role of GST as prognostic indicator of alcohol abstinence with possible trend towards an improvement in liver function

Model-based Segmentation and Deep Learning for Lung Cancer

Lung cancer is one of the life taking disease and causes more deaths worldwide. Early detection and treatment is necessary to save life. It is very difficult for doctors to interpret and identify diseases using imaging modalities alone. Therefore computer aided diagnosis can assist doctors for the early detection of cancer very accurately. In the proposed work, optimized deformable models and deep learning techniques are applied for the detection and classification of lung cancer. This method involves pre-processing, lung lobe segmentation, lung cancer segmentation, Data augmentation and lung cancer classification. The median filtering is considered for pre-processing and the Bayesian fuzzy clustering is applied for segmenting the lung lobes. The lung cancer segmentation is carried out using Water Cycle Sea Lion Optimization (WSLnO) based deformable model. The data augmentation process is used to augment the size of segmented region in order to perform better classification. The lung cancer classification is done effectively using Shepard Convolutional Neural Network (ShCNN), which is trained by WSLnO algorithm. The proposed WSLnO algorithm is designed by incorporating Water cycle algorithm (WCA) and Sea Lion Optimization (SLnO) algorithm. The performance of the proposed technique is analyzed with various performance metrics and attained the better results in terms of accuracy, sensitivity, specificity and average segmentation accuracy of 0.9303, 0.9123, 0.9133 and 0.9091 respectively

Acute aortic syndromes in India: the need for a nationwide program to increase awareness

Acute aortic dissection is a rare, but potentially life-threatening and time-critical condition that is frequently misdiagnosed. Therefore, its prompt and proper diagnosis is vital to increase a patient's chance of survival and to prevent grievous complications. Raising awareness and educating the general population and healthcare professionals about an aortic dissection is mandatory, for early diagnosis and improving the chances of survival.Thoracic Surger