13 research outputs found
A fiber matrix model for the filtration through fenestral pores in a compressible arterial intima
A Fiber Matrix Model for the Growth of Macromolecular Leakage Spots in the Arterial Intima
The double layer–capillary stability of an annular electrolyte fluid surrounding a dielectric-fluid core in a tube
A model for the initiation and growth of extracellular lipid liposomes in arterial intima
An asymptotic theory for the linear stability of a core–annular flow in the thin annular limit
Quantification of the plasma clearance kinetics of a gadolinium-based contrast agent by photoinduced triplet harvesting.
The use of gadolinium-based contrast agents (GBCA) is integral to the field of diagnostic magnetic resonance imaging (MRI). Pharmacokinetic evaluation of the plasma clearance of GBCA is required for all new agents or improved formulations, to address concerns over toxicity or unforeseen side effects. Current methods to measure GBCA in plasma lack either a rapid readout or the sensitivity to measure small samples or require extensive processing of plasma, all obstacles in the development and characterization of new GBCA. Here, we quantify the plasma concentration of a labeled analogue of a common clinical GBCA by ligand triplet harvesting and energy transfer. The nonemittive GBCA becomes a "dark donor" to a fluorescent detector molecule, with a lower limit of detection of 10(-7) M in unprocessed plasma. On a time scale of minutes, we determine the plasma clearance rate in the wild-type mouse, using time-resolved fluorescence on a standard laboratory plate reader