975 research outputs found
A multi-dimensional approach from seed-to-seed to understand and improve heat stress tolerance in rice
In changing climatic conditions, stress caused by high temperature
poses a serious threat to rice cultivation. Physiological,
biochemical, and molecular analysis of rice cultivars revealed
that Nagina22 (N22) shows lesser reduction in chlorophyll content,
net photosynthetic rate, spikelet fertility and grain yield,
but increased membrane thermal stability, antioxidant enzymes
activity and transpiration rate (E) at high temperature. DREB,
RAB, LEA, and genes associated with hormones signalling were
induced during germination, while OsFd (an iron sulphur cluster
binding protein) and CWIP (cell wall integrity protein) emerged
as high priority candidate genes in seedling and reproductive
stages. Their function is being analysed by transgene expression
and CRISPR/Cas genome editing approaches. Field screening
in polyhouse, late sowing and temperature gradient chamber
for 20 morpho-physiological traits indicated the importance of
both yield and spikelet fertility, and photosynthesis traits. N22
showed the least Heat Susceptibility Index (HSI) for yield/plant,
spikelet fertility, flag leaf SPAD and stomatal conductance, while
Vandana showed the highest HSI for spikelet fertility and flag leaf
temperature. QTLs for HSI of spikelet fertility were identified on
chromosome 1 and HSI of yield per plant on chromosomes 1, 2, 3,
4, 7 and 8; and PV of 6% to 57% using 174 F2-3 Vandana x N22
mapping population. Simultaneously, RNAseq was performed
to identify the genome wide miRNAs and transcriptome of N22
and Vandana from shoot and root after short and long duration
of heat stress treatments; and recovery phase for an eQTL-guided
function-related co-expression analysis to identify the putative
regulators and gene regulatory networks
Insilico and Invitro anthelmintic properties of phytocompounds in Rostellularia quinquangularis (J. Koenig ex Roxb.) Nees
The present study aimed to evaluate the anthelmintic activity of various extracts of Rostellularia quinquangularis (R. quinquangularis) against adult Indian earthworms (Pheretima posthuma). Petroleum ether extract (PERQ), ethyl acetate extract (RQEA), and ethanol extract (RQEE) of R. quinquangularis were tested at different concentrations (10, 20, 50, and 100 mg/mL), along with the positive control (albendazole) and negative control (normal saline). Anthelmintic activity was assessed based on the duration of paralysis and mortality. The RQEE extract showed significant anthelmintic activity, with the highest activity observed at a concentration of 100 mg/mL, exhibiting paralysis time of 1.62 min and death times of 19.9 min, compared to the standard albendazole. Further, HR LC-MS analysis of the RQEE extract revealed the presence of various phytoconstituents based on m/z signals. Molecular docking analysis using AutoDock Vina indicated that Columbianetin, Dunnione, Cryptochlorogenic acid, Gaylussacin, Luvangetin, and Albendazole showed docking scores of -8.1, -7.9, -7.4, -7.3, -7.2, and -6.8 Kcal/mol, respectively. These results suggest that R. quinquangularis possesses potent anthelmintic activity, supporting its traditional use in medicinal practices.
Deep sequencing of small RNAs reveals ribosomal origin of microRNAs in Oryza sativa and their regulatory role in high temperature
MicroRNAs are small noncoding regulatory RNAs which control gene expression by mRNA degradation or translational repression. They are significant molecular players regulating important biological processes such as developmental timing and stress response. We report here the discovery of miRNAs derived from ribosomal DNA using the small RNA datasets of 16 deep sequencing libraries of rice. Twelve putative miRNAs were identified based on highly stringent criteria of novel miRNA prediction. Surprisingly, 10 putative miRNAs (mi_7403, mi_8435, mi_12675, mi_4266, mi_4758, mi_4218, mi_8200, mi_4644, mi_14291, mi_16235) originated from rDNA of rice chromosome 9. Expression analysis of putative miRNAs and their target genes in heat tolerant and susceptible rice cultivars in control and high temperature treated seedlings revealed differential regulation of rDNA derived miRNAs. This is the first report of rDNA derived miRNAs in rice which indicates their role in gene regulation during high temperature stress in plants. Further studies in this area will open new research challenges and opportunities to broaden our knowledge on gene regulation mechanisms
Observation of and search for violation in radiative charm decays
We report the first observation of the radiative charm decay and the first search for violation in decays , , and , using a data sample of
943 fb collected with the Belle detector at the KEKB asymmetric-energy
collider. The branching fraction is measured to be , where the first
uncertainty is statistical and the second is systematic. The obtained
asymmetries, , , and
, are consistent with no violation. We also present an improved
measurement of the branching fractions and
Search for violation in the decay at Belle
We search for violation in the charged charm meson decay
, based on a data sample corresponding to an integrated
luminosity of collected by the Belle experiment at the KEKB
asymmetric-energy collider. The measured violating asymmetry
is , which is consistent with
the standard model prediction and has a significantly improved precision
compared to previous results.Comment: 8 pages, 3 figure
Evidence for Isospin Violation and Measurement of Asymmetries in
We report the first evidence for isospin violation in and
the first measurement of difference of asymmetries between and . This analysis is based on the data sample
containing pairs that was collected with the Belle
detector at the KEKB energy-asymmetric collider. We find evidence for
the isospin violation with a significance of 3.1, \%, where
the third uncertainty is due to the uncertainty on the fraction of to
production in decays. The measured value is
consistent with predictions of the SM. The result for the difference of
asymmetries is \%, consistent with zero. The measured branching fractions and
asymmetries for charged and neutral meson decays are the most precise to
date. We also calculate the ratio of branching fractions of to .Comment: 11 pages, 7 figures. shown at FPCP2017. accepted by PR
Angular analysis of
We present a measurement of angular observables, , , ,
, in the decay , where
is either or . The analysis is performed on
a data sample corresponding to an integrated luminosity of
containing pairs, collected
at the resonance with the Belle detector at the
asymmetric-energy collider KEKB. Four angular observables,
are extracted in five bins of the invariant mass squared of the
lepton system, . We compare our results for with Standard
Model predictions including the region in which the LHCb collaboration
reported the so-called anomaly.Comment: Conference paper for LHC Ski 2016. SM prediction for
corrected and reference for arXiv:1207.2753 adde
Measurement of the lepton polarization and in the decay
We report the first measurement of the lepton polarization
in the decay as
well as a new measurement of the ratio of the branching fractions , where
denotes an electron or a muon, and the is reconstructed in the modes
and .
We use the full data sample of pairs recorded
with the Belle detector at the KEKB electron-positron collider. Our results,
and
, are
consistent with the theoretical predictions of the Standard Model.Comment: 7 pages, 2 figures, submitted to Physical Review Letters; one figure
was removed from the first versio
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