Sensitive detection and glycoprofiling of a prostate specific antigen using impedimetric assays

This study presents a proof-of-concept for the development of an impedimetric biosensor for ultra-sensitive glycoprofiling of prostate specific antigen (PSA). The biosensor exhibits three unique characteristics: (1) analysis of PSA with limit of detection (LOD) down to 4 aM; (2) analysis of the glycan part of PSA with LOD down to 4 aM level and; (3) both assays (i.e., PSA quantification and PSA glycoprofiling) can be performed on the same interface due to label-free analysis.The financial support received from the Slovak Scientific Grant Agency VEGA 2/0162/14 and 1/0229/12; and from the Slovak Research and Development Agency APVV 0282-11 is acknowledged. This report was made possible by a NPRP award [NPRP grant no. 6-381-1-078] from the Qatar National Research Fund (a member of the Qatar Foundation). The statements made herein are solely the responsibility of the authors. The research leading to these results received funding from the European Research Council under the European Union?s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement no. 311532 and this work has received funding from the European Union?s Seventh Framework Program for research, technological development and demonstration under grant agreement no. 317420. This publication is the result of the project implementation: Centre for materials, layers and systems for applications and chemical processes under extreme conditions ? Stage I, ITMS No.: 26240120007 supported by the Research & Development Operational Programme funded by the ERDF.Scopu