261 research outputs found
Establishment of the Coast Range ophiolite microbial observatory (CROMO): Drilling objectives and preliminary outcomes
Abstract. This project aimed to establish a subsurface microbial observatory in ultramafic rocks, by drilling into an actively serpentinizing peridotite body, characterizing cored rocks, and outfitting the boreholes for a program of long-term observation and experimentation to resolve the serpentinite-hosted subsurface biosphere. We completed drilling in August 2011, drilling two boreholes with core recovery and possibility for down-hole experimentation, and six smaller-diameter monitoring wells arrayed around the two primary holes, in the Coast Range ophiolite (CRO) locality in the UC-Davis McLaughlin Natural Reserve, Lower Lake, CA. Every effort was made during drilling to keep the cores and wells as free of drilling-induced contamination as possible: clean, purified water was used as drilling fluid, fluorescent microbead tracers were suspended in that water for quantification of drilling fluid penetration into the cores, and high resolution next generation sequencing approaches were used to characterize the microbial populations in the drill fluids and core materials. In December 2011, we completed installation of well pumps (slow flow bladder pumps) in the monitoring wells, and have deployed a set of in situ incubation experiments in the two uncased boreholes. Preliminary findings illustrate natural variability in actively serpentinizing strata, and confirm distinct groundwater flow regimes and microbial ecosystems in (a) shallow, surface-impacted soil water horizons and (b) deeper, ultramafic bedrock-sourced formation fluids
Carbon assimilation strategies in ultrabasic groundwater: clues from the integrated study of a serpentinization-influenced aquifer
© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Seyler, L. M., Brazelton, W. J., McLean, C., Putman, L. I., Hyer, A., Kubo, M. D. Y., Hoehler, T., Cardace, D., & Schrenk, M. O. . Carbon assimilation strategies in ultrabasic groundwater: clues from the integrated study of a serpentinization-influenced aquifer. mSystems, 5(2), (2020): e00607-00619, doi: 10.1128/mSystems.00607-19.Serpentinization is a low-temperature metamorphic process by which ultramafic rock chemically reacts with water. Such reactions provide energy and materials that may be harnessed by chemosynthetic microbial communities at hydrothermal springs and in the subsurface. However, the biogeochemistry mediated by microbial populations that inhabit these environments is understudied and complicated by overlapping biotic and abiotic processes. We applied metagenomics, metatranscriptomics, and untargeted metabolomics techniques to environmental samples taken from the Coast Range Ophiolite Microbial Observatory (CROMO), a subsurface observatory consisting of 12 wells drilled into the ultramafic and serpentinite mélange of the Coast Range Ophiolite in California. Using a combination of DNA and RNA sequence data and mass spectrometry data, we found evidence for several carbon fixation and assimilation strategies, including the Calvin-Benson-Bassham cycle, the reverse tricarboxylic acid cycle, the reductive acetyl coenzyme A (acetyl-CoA) pathway, and methylotrophy, in the microbial communities inhabiting the serpentinite-hosted aquifer. Our data also suggest that the microbial inhabitants of CROMO use products of the serpentinization process, including methane and formate, as carbon sources in a hyperalkaline environment where dissolved inorganic carbon is unavailable.We thank McLaughlin Reserve, in particular Paul Aigner and Cathy Koehler, for hosting sampling at CROMO and providing access to the wells, A. Daniel Jones and Anthony Schilmiller for their advice regarding metabolite extraction and mass spectrometry, Elizabeth Kujawinski for her guidance in metabolomics data analysis and interpretation, and Julia McGonigle, Christopher Thornton, and Katrina Twing for assistance with metagenomic and computational analyses
Pion parameters in nuclear medium from chiral perturbation theory and virial expansion
We consider two methods to find the effective parameters of the pion
traversing a nuclear medium. One is the first order chiral perturbation
theoretic evaluation of the pion pole contribution to the two-point function of
the axial-vector current. The other is the exact, first order virial expansion
of the pion self-energy. We find that, although the results of chiral
perturbation theory are not valid at normal nuclear density, those from the
virial expansion may be reliable at such density. The latter predicts both the
mass-shift and the in-medium decay width of the pion to be small, of about a
few MeV.Comment: 9 Pages RevTex, 3 eps figure
Factors that control the stable carbon isotopic composition of methane produced in an anoxic marine sediment
The carbon isotopic composition of methane produced in anoxic marine sediment is controlled by four factors: (1) the pathway of methane formation, (2) the isotopic composition of the methanogenic precursors, (3) the isotope fractionation factors for methane production, and (4) the isotope fractionation associated with methane oxidation. The importance of each factor was evaluated by monitoring stable carbon isotope ratios in methane produced by a sediment microcosm. Methane did not accumulate during the initial 42-day period when sediment contained sulfate, indicating little methane production from 'noncompetitive' substrates. Following sulfate depletion, methane accumulation proceeded in three distinct phases. First, CO2 reduction was the dominant methanogenic pathway and the isotopic composition of the methane produced ranged from -80 to -94 per thousand. The acetate concentration increased during this phase, suggesting that acetoclastic methanogenic bacteria were unable to keep pace with acetate production. Second, acetate fermentation became the dominant methanogenic pathway as bacteria responded to elevated acetate concentrations. The methane produced during this phase was progressively enriched in C-13, reaching a maximum delta(C-13) value of -42 per thousand. Third, the acetate pool experienced a precipitous decline from greater than 5 mM to less than 20 micro-M and methane production was again dominated by CO2 reduction. The delta(C-13) of methane produced during this final phase ranged from -46 to -58 per thousand. Methane oxidation concurrent with methane production was detected throughout the period of methane accumulation, at rates equivalent to 1 to 8 percent of the gross methane production rate. Thus methane oxidation was too slow to have significantly modified the isotopic signature of methane. A comparison of microcosm and field data suggests that similar microbial interactions may control seasonal variability in the isotopic composition of methane emitted from undisturbed Cape Lookout Bight sediment
Metabolic variability in seafloor brines revealed by carbon and sulphur dynamics
Brine fluids that upwell from deep, hot reservoirs below the sea bed supply the sea floor with energy-rich substrates and nutrients that are used by diverse microbial ecosystems. Contemporary hypersaline environments formed by brine seeps may provide insights into the metabolism and distribution of microorganisms on the early Earth or on extraterrestrial bodies. Here we use geochemical and genetic analyses to characterize microbial community composition and metabolism in two seafloor brines in the Gulf of Mexico: an active mud volcano and a quiescent brine pool. Both brine environments are anoxic and hypersaline. However, rates of sulphate reduction and acetate production are much higher in the brine pool, whereas the mud volcano supports much higher rates of methane production. We find no evidence of anaerobic oxidation of methane, despite high methane fluxes at both sites. We conclude that the contrasting microbial community compositions and metabolisms are linked to differences in dissolved-organic-matter input from the deep subsurface and different fluid advection rates between the two sites.
DOI: 10.1038/NGEO47
Neutron charge form factor at large
The neutron charge form factor is determined from an analysis of
the deuteron quadrupole form factor data. Recent calculations, based
on a variety of different model interactions and currents, indicate that the
contributions associated with the uncertain two-body operators of shorter range
are relatively small for , even at large momentum transfer . Hence,
can be extracted from at large without undue
systematic uncertainties from theory.Comment: 8 pages, 3 figure
The Decuplet Revisited in PT
The paper deals with two issues. First, we explore the quantitiative
importance of higher multiplets for properties of the decuplet in
chiral perturbation theory. In particular, it is found that the lowest order
one--loop contributions from the Roper octet to the decuplet masses and
magnetic moments are substantial. The relevance of these results to the chiral
expansion in general is discussed. The exact values of the magnetic moments
depend upon delicate cancellations involving ill--determined coupling
constants. Second, we present new relations between the magnetic moments of the
decuplet that are independent of all couplings. They are exact at the
order of the chiral expansion used in this paper.Comment: 7 pages of double column revtex, no figure
A model for two-proton emission induced by electron scattering
A model to study two-proton emission processes induced by electron scattering
is developed. The process is induced by one-body electromagnetic operators
acting together with short-range correlations, and by two-body
currents. The model includes all the diagrams containing a single correlation
function. A test of the sensitivity of the model to the various theoretical
inputs is done. An investigation of the relevance of the currents is
done by changing the final state angular momentum, excitation energy and
momentum transfer. The sensitivity of the cross section to the details of the
correlation function is studied by using realistic and schematic correlations.
Results for C, O and Ca nuclei are presented.Comment: 30 pages, 18 figures, 3 table
Covariant and Heavy Quark Symmetric Quark Models
There exist relativistic quark models (potential or MIT-bag) which satisfy
the heavy quark symmetry (HQS) relations among meson decay constants and form
factors. Covariant construction of the momentum eigenstates, developed here,
can correct for spurious center-of-mass motion contributions.Proton form factor
and M1 transitions in quarkonia are calculated. Explicit expression for the
Isgur-Wise function is found and model determined deviations from HQS are
studied. All results depend on the model parameters only. No additional ad hoc
assumptions are needed.Comment: 34 pages (2 figures not included but avaliable upon request), LATEX,
(to be published in Phys.Rev.D
- …