LYME DISEASE. CLINICAL AND EPIDEMIOLOGICAL STUDY FOR THE PERIOD 1989-1991 IN VARNA REGION

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ACTIVATION OF PHAGOCYTOSIS IN VITRO WITH FRACTIONS OF FASEOLOSAXIN

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Does BCR/ABL1 positive Acute Myeloid Leukaemia Exist?

The BCR/ABL1 fusion gene, usually carried by the Philadelphia chromosome (Ph) resulting from t(9;22)(q34;q11) or variants, is pathognomonic for chronic myeloid leukaemia (CML). It is also occasionally found in acute lymphoblastic leukaemia (ALL) mostly in adults and rarely in de novo acute myeloid leukaemia (AML). Array Comparative Genomic Hybridization (aCGH) was used to study six Ph(+)AML, three bi-lineage and four Ph(+)ALL searching for specific genomic profiles. Surprisingly, loss of the IKZF1 and/or CDKN2A genes, the hallmark of Ph(+)ALL, were recurrent findings in Ph(+)AML and accompanied cryptic deletions within the immunoglobulin and T cell receptor genes. The latter two losses have been shown to be part of 'hot spot' genome imbalances associated with BCR/ABL1 positive pre-B lymphoid phenotype in CML and Ph(+)ALL. We applied Significance Analysis of Microarrays (SAM) to data from the 'hot spot' regions to the Ph(+)AML and a further 40 BCR/ABL1(+) samples looking for differentiating features. After exclusion of the most dominant markers, SAM identified aberrations unique to de novo Ph(+)AML that involved relevant genes. While the biological and clinical significance of this specific genome signature remains to be uncovered, the unique loss within the immunoglobulin genes provides a simple test to enable the differentiation of clinically similar de novo Ph(+) AML and myeloid blast crisis of CML. © 2013 John Wiley & Sons Ltd and Crown

CHANGES IN THE ACTIVITY OF SOME SERUM ENZYMES IN PATIENTS WITH EXTRAHEPATIC CHOLESTASIS

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Music and Technics: the understanding of our aesthetic experience before and after the Industrial Revolution considered through the eyes of Arthur Schopenhauer and Bernard Stiegler

Two centuries ago Schopenhauer claimed that through music people were able to escape from what he defined as an evil world, but music is created and performed in a different way today, especially after the Industrial Revolution. Not only were there new technological inventions but also through them some secrets about humanity were revealed, secrets which influence the change in our understanding of what is called aesthetic experience, namely that we are not only biological and social, but also technical creatures. This is the so-called by Bernard Stiegler general organology. If this organological connection gets disrupted we start losing our individuation. Technic itself, however, cannot be blamed for this disindividuation since it is a pharmakon. The digital era we live in can be seen as a negative outcome of the use of technics but in this paper the opportunities of the healthy relation with technics will be elaborated on

CHANGES IN THE ACTIVITY OF SOME SERUM ENZYMES IN CHRONI CLIVER DISEASES

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HIGH REMISSION AND LOW RECURRENCE RATES OF HIDRADENITIS SUPPURATIVA LESIONS TREATED BY REGIONAL COMPLETE EXCISION AND SECONDARY INTENTION WOUND HEALING AFTER36 MONTHS OF FOLLOW-UP

Hidradenitis suppurativa (HS) is a chronic inflammatory disease which mostly affects intertriginous skin areas. For its advanced stage, regional complete surgical excision (CSE), followed by healing by secondary intention is a useful treatment modality. PURPOSE: We performed a pilot case series study to calculate remission and defined recurrence rates of HS lesions after regional CSE. METHODS: 44 regional CSE were performed in 22 patients with Hurley stage II/III disease. The mean resulting wound size was 96 cm². After completed reepithelization by secondary intention, patients were under regular face-to-face follow-up for 36 months at the Clinic of Dermatology and VenereologyinStara Zagora. New HS lesions within 1 cm around the resulting scar were rated as regional recurrence. RESULTS: 42/44 (95.5%) excision sites showed complete scarring without hypertrophic scar, keloid formation, functional impairment or lymphedema. Regional recurrence was detected in 2/44 (4.5%) excision sites. New HS lesions distant from the regional excision site developed in 1/22 (4.5%) patients. CONCLUSIONS: Our results underscorethe high remission and low defined recurrence rates of treatment of advanced HS lesions by regional CSE and secondary intention wound healing and further support the significance of this treatment modality for long-term disease control

THE CASE OF CUTANEOUS ANTHRAX

A case of the disease in the skin form of anthrax which was held with discrete toksoinfektsionnym syndrome, but with characteristic skin manifestations, etiology, and ended proven recovery

Optimization and Characterization of N-Acetamide Indoles as Antimalarials That Target PfATP4

To discover new antimalarials, a screen of the Janssen Jumpstarter library against Plasmodium falciparum uncovered the N-acetamide indole hit class. The structure-activity relationship of this chemotype was defined and culminated in the optimized frontrunner analog WJM664, which exhibited potent asexual stage activity and high metabolic stability. Resistant selection and whole-genome sequencing revealed mutations in PfATP4, which was validated as the target by showing that analogs exhibited reduced potency against parasites with resistance-conferring mutations in PfATP4, a metabolomic signature similar to that of the PfATP4 inhibitor KAE609, and inhibition of Na+-dependent ATPase activity consistent with on-target inhibition of PfATP4. WJM664 inhibited gamete development and blocked parasite transmission to mosquitoes but exhibited low efficacy in aPlasmodium berghei mouse model, which was attributed to ATP4 species differentiation and its moderate systemic exposure. Optimization of these attributes is required for N-acetamide indoles to be pursued for development as a curative and transmission-blocking therapy.</p

International Consensus Based Review and Recommendations for Minimum Reporting Standards in Research on Transcutaneous Vagus Nerve Stimulation (Version 2020).

Given its non-invasive nature, there is increasing interest in the use of transcutaneous vagus nerve stimulation (tVNS) across basic, translational and clinical research. Contemporaneously, tVNS can be achieved by stimulating either the auricular branch or the cervical bundle of the vagus nerve, referred to as transcutaneous auricular vagus nerve stimulation(VNS) and transcutaneous cervical VNS, respectively. In order to advance the field in a systematic manner, studies using these technologies need to adequately report sufficient methodological detail to enable comparison of results between studies, replication of studies, as well as enhancing study participant safety. We systematically reviewed the existing tVNS literature to evaluate current reporting practices. Based on this review, and consensus among participating authors, we propose a set of minimal reporting items to guide future tVNS studies. The suggested items address specific technical aspects of the device and stimulation parameters. We also cover general recommendations including inclusion and exclusion criteria for participants, outcome parameters and the detailed reporting of side effects. Furthermore, we review strategies used to identify the optimal stimulation parameters for a given research setting and summarize ongoing developments in animal research with potential implications for the application of tVNS in humans. Finally, we discuss the potential of tVNS in future research as well as the associated challenges across several disciplines in research and clinical practice