Organic Acid and DNA Sensing with Electrochemical Sensor Based on Carbon Black and Pillar[5]arene

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimElectrochemical sensor has been proposed on the base of glassy carbon electrode (GCE) modified with carbon black (CB) and pillar[5]arene (P[5]A). The characteristics of the peak currents were found to be sensitive to the incubation of the sensor in organic acid and DNA solution. The detection of n×(10−8–10−5) M of organic acid and down to (1–5)×10−18 g of DNA was attributed to the effect of the analytes on the aggregation and relative stability of oxidized and reduced forms of P[5]A. No signal interference with the alkali and alkali-earth metal ions was found. The electrochemical sensor was tested in the detection of specific DNA interactions, i.e. reactive oxygen species damage and intercalating pharmaceuticals detection

Electrochemical behavior of pillar[5]arene on glassy carbon electrode and its interaction with Cu2+ and Ag+ ions

© 2014 Elsevier Ltd. All rights reserved. The electrochemical behavior of pillar[5]arene (P[5]A) and of its reaction products with Ag+ and Cu2+ ions has been investigated using cyclic voltammetry, optical methods and transmission electron microscopy (TEM). Stepwise oxidation of hydroquinone units of P[5]A molecule is guided by self-assembling and acid-base interactions. From one to three hydroquinone units per P[5]A molecule are oxidized depending on the measurement conditions. The deposition of P[5]A on glassy carbon electrode (GCE) partially blocks the electron transduction. Interfering influence of dissolved oxygen can be partially eliminated by the use of carbon black as immobilization matrix. The reaction of P[5]A with silver ions results in formation of most stable form with three benzoquinone and two hydroquinone units stabilized by quinhydrone-like structure. The Ag nanoparticles formed in the reaction retain electron transduction with the electrode due to involvement of shielding P[5]A molecules. Similar reaction with Cu2+ ions does not lead to stable products because of the formation of Cu2O particles detected by UV spectroscopy and TEM. Possible analytical applications of the materials obtained were proved by electrocatalytic reduction of hydrogen peroxide and mediated oxidation of thiocholine as model systems. In both cases, high sensitivity and wide range of the concentration determined were shown

К ВОПРОСУ ОБ ИШЕМИИ СТВОЛА МОЗГА И ХРОНИЗАЦИИ ЛЮМБОИШИАЛГИИ

We analyzed 60 patients aged from 30 up to 75 years with any of low-lumbar herniated intervertebral disks. Pain intensity was compared with the concomitant presence of chronic circulatory failure in the vertebral-basilar basin as a result of clinically significant abnormalities of blood vessels in the form of hypoplasia of vertebral artery and its tortuosity. We investigated dynamics of patient's pain under the influence of low-dose treatment by Cortexin (neurocytoprotector). In patients with chronic brainstem ischemia the herniated discs cause more intense and prolonged pain (radicular and local). More effective in these patients is a treatment with inclusion of Cortexin 20 mg intramuscularly within 10 days.Анализируется 60 больных в возрасте от 30 до 75 лет с грыжами одного из нижнепоясничных межпозвонковых дисков. Оценивается интенсивность болевого синдрома в сопоставлении с сопутствующим наличием хронической недостаточности кровообращения в вертебрально-базилярном бассейне вследствие клинически значимой аномалии сосудов в виде гипоплазии позвоночной артерии или ее извитости. Изучена также динамика боли у этих пациентов под влиянием лечения низкодозированным нейроцитопротектором кортексином. У пациентов с хронической ишемией ствола мозга грыжи дисков вызывают более интенсивную и продолжительную боль (корешковую и локальную). Эффективным у таких пациентов является лечебный комплекс с включением кортексина по 20 мг внутримышечно в течение 10 дней

Organic Acid and DNA Sensing with Electrochemical Sensor Based on Carbon Black and Pillar[5]arene

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimElectrochemical sensor has been proposed on the base of glassy carbon electrode (GCE) modified with carbon black (CB) and pillar[5]arene (P[5]A). The characteristics of the peak currents were found to be sensitive to the incubation of the sensor in organic acid and DNA solution. The detection of n×(10−8–10−5) M of organic acid and down to (1–5)×10−18 g of DNA was attributed to the effect of the analytes on the aggregation and relative stability of oxidized and reduced forms of P[5]A. No signal interference with the alkali and alkali-earth metal ions was found. The electrochemical sensor was tested in the detection of specific DNA interactions, i.e. reactive oxygen species damage and intercalating pharmaceuticals detection

Organic Acid and DNA Sensing with Electrochemical Sensor Based on Carbon Black and Pillar[5]arene

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimElectrochemical sensor has been proposed on the base of glassy carbon electrode (GCE) modified with carbon black (CB) and pillar[5]arene (P[5]A). The characteristics of the peak currents were found to be sensitive to the incubation of the sensor in organic acid and DNA solution. The detection of n×(10−8–10−5) M of organic acid and down to (1–5)×10−18 g of DNA was attributed to the effect of the analytes on the aggregation and relative stability of oxidized and reduced forms of P[5]A. No signal interference with the alkali and alkali-earth metal ions was found. The electrochemical sensor was tested in the detection of specific DNA interactions, i.e. reactive oxygen species damage and intercalating pharmaceuticals detection

Organic Acid and DNA Sensing with Electrochemical Sensor Based on Carbon Black and Pillar[5]arene

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimElectrochemical sensor has been proposed on the base of glassy carbon electrode (GCE) modified with carbon black (CB) and pillar[5]arene (P[5]A). The characteristics of the peak currents were found to be sensitive to the incubation of the sensor in organic acid and DNA solution. The detection of n×(10−8–10−5) M of organic acid and down to (1–5)×10−18 g of DNA was attributed to the effect of the analytes on the aggregation and relative stability of oxidized and reduced forms of P[5]A. No signal interference with the alkali and alkali-earth metal ions was found. The electrochemical sensor was tested in the detection of specific DNA interactions, i.e. reactive oxygen species damage and intercalating pharmaceuticals detection

Electrochemical behavior of pillar[5]arene on glassy carbon electrode and its interaction with Cu2+ and Ag+ ions

© 2014 Elsevier Ltd. All rights reserved. The electrochemical behavior of pillar[5]arene (P[5]A) and of its reaction products with Ag+ and Cu2+ ions has been investigated using cyclic voltammetry, optical methods and transmission electron microscopy (TEM). Stepwise oxidation of hydroquinone units of P[5]A molecule is guided by self-assembling and acid-base interactions. From one to three hydroquinone units per P[5]A molecule are oxidized depending on the measurement conditions. The deposition of P[5]A on glassy carbon electrode (GCE) partially blocks the electron transduction. Interfering influence of dissolved oxygen can be partially eliminated by the use of carbon black as immobilization matrix. The reaction of P[5]A with silver ions results in formation of most stable form with three benzoquinone and two hydroquinone units stabilized by quinhydrone-like structure. The Ag nanoparticles formed in the reaction retain electron transduction with the electrode due to involvement of shielding P[5]A molecules. Similar reaction with Cu2+ ions does not lead to stable products because of the formation of Cu2O particles detected by UV spectroscopy and TEM. Possible analytical applications of the materials obtained were proved by electrocatalytic reduction of hydrogen peroxide and mediated oxidation of thiocholine as model systems. In both cases, high sensitivity and wide range of the concentration determined were shown

Electrochemical behavior of pillar[5]arene on glassy carbon electrode and its interaction with Cu2+ and Ag+ ions

© 2014 Elsevier Ltd. All rights reserved. The electrochemical behavior of pillar[5]arene (P[5]A) and of its reaction products with Ag+ and Cu2+ ions has been investigated using cyclic voltammetry, optical methods and transmission electron microscopy (TEM). Stepwise oxidation of hydroquinone units of P[5]A molecule is guided by self-assembling and acid-base interactions. From one to three hydroquinone units per P[5]A molecule are oxidized depending on the measurement conditions. The deposition of P[5]A on glassy carbon electrode (GCE) partially blocks the electron transduction. Interfering influence of dissolved oxygen can be partially eliminated by the use of carbon black as immobilization matrix. The reaction of P[5]A with silver ions results in formation of most stable form with three benzoquinone and two hydroquinone units stabilized by quinhydrone-like structure. The Ag nanoparticles formed in the reaction retain electron transduction with the electrode due to involvement of shielding P[5]A molecules. Similar reaction with Cu2+ ions does not lead to stable products because of the formation of Cu2O particles detected by UV spectroscopy and TEM. Possible analytical applications of the materials obtained were proved by electrocatalytic reduction of hydrogen peroxide and mediated oxidation of thiocholine as model systems. In both cases, high sensitivity and wide range of the concentration determined were shown

Electrochemical behavior of pillar[5]arene on glassy carbon electrode and its interaction with Cu2+ and Ag+ ions

© 2014 Elsevier Ltd. All rights reserved. The electrochemical behavior of pillar[5]arene (P[5]A) and of its reaction products with Ag+ and Cu2+ ions has been investigated using cyclic voltammetry, optical methods and transmission electron microscopy (TEM). Stepwise oxidation of hydroquinone units of P[5]A molecule is guided by self-assembling and acid-base interactions. From one to three hydroquinone units per P[5]A molecule are oxidized depending on the measurement conditions. The deposition of P[5]A on glassy carbon electrode (GCE) partially blocks the electron transduction. Interfering influence of dissolved oxygen can be partially eliminated by the use of carbon black as immobilization matrix. The reaction of P[5]A with silver ions results in formation of most stable form with three benzoquinone and two hydroquinone units stabilized by quinhydrone-like structure. The Ag nanoparticles formed in the reaction retain electron transduction with the electrode due to involvement of shielding P[5]A molecules. Similar reaction with Cu2+ ions does not lead to stable products because of the formation of Cu2O particles detected by UV spectroscopy and TEM. Possible analytical applications of the materials obtained were proved by electrocatalytic reduction of hydrogen peroxide and mediated oxidation of thiocholine as model systems. In both cases, high sensitivity and wide range of the concentration determined were shown