ELAV links paused Pol II to alternative polyadenylation in the Drosophila nervious system

Alternative polyadenylation (APA) has been implicated in a variety of developmental and disease processes. A particularly dramatic form of APA occurs in the developing nervous system of flies and mammals, whereby various developmental genes undergo coordinate 3' UTR extension. In Drosophila, the RNA-binding protein ELAV inhibits RNA processing at proximal polyadenylation sites, thereby fostering the formation of exceptionally long 3' UTRs. Here, we present evidence that paused Pol II promotes recruitment of ELAV to extended genes. Replacing promoters of extended genes with heterologous promoters blocks normal 3' extension in the nervous system, while extension-associated promoters can induce 3' extension in ectopic tissues expressing ELAV. Computational analyses suggest that promoter regions of extended genes tend to contain paused Pol II and associated cis-regulatory elements such as GAGA. ChIP-seq assays identify ELAV in the promoter regions of extended genes. Our study provides evidence for a regulatory link between promoter-proximal pausing and APA

Effects of canola, wheat and faba bean on yield and nitrogen use efficiency in potato production systems

Non-Peer Reviewe

Vanishing Fe 3d orbital moments in single-crystalline magnetite

We show detailed magnetic absorption spectroscopy results of an in situ cleaved high quality single crystal of magnetite. In addition the experimental setup was carefully optimized to reduce drift, self absorption, and offset phenomena as far as possible. In strong contradiction to recently published data, our observed orbital moments are nearly vanishing and the spin moments are quite close to the integer values proposed by theory. This very important issue supports the half metallic full spin polarized picture of magnetite.Comment: 7 pages, 4 figure

Deep far infrared ISOPHOT survey in "Selected Area 57", I. Observations and source counts

We present here the results of a deep survey in a 0.4 sq.deg. blank field in Selected Area 57 conducted with the ISOPHOT instrument aboard ESAs Infrared Space Observatory (ISO) at both 60 um and 90 um. The resulting sky maps have a spatial resolution of 15 x 23 sq.arcsec. per pixel which is much higher than the 90 x 90 sq.arcsec. pixels of the IRAS All Sky Survey. We describe the main instrumental effects encountered in our data, outline our data reduction and analysis scheme and present astrometry and photometry of the detected point sources. With a formal signal to noise ratio of 6.75 we have source detection limits of 90 mJy at 60 um and 50 mJy at 90 um. To these limits we find cumulated number densities of 5+-3.5 per sq.deg. at 60 um and 14.8+-5.0 per sq.deg.at 90 um. These number densities of sources are found to be lower than previously reported results from ISO but the data do not allow us to discriminate between no-evolution scenarios and various evolutionary models.Comment: 15 pages, 11 figures, accepted by Astronomy & Astrophysic

Stomatin-like Protein 2 Links Mitochondria to T-Cell Receptor Signalosomes at the Immunological Synapse and Enhances T-Cell Activation

T cell activation through the antigen receptor (TCR) requires sustained signalling from microclusters in the peripheral region of the immunological synapse (IS). The bioenergetics of such prolonged signaling have been linked to the redistribution of mitochondria to the IS. Here, we report that stomatin-like protein-2 (SLP-2) plays an important role in this process by bridging polarized mitochondria to these signaling TCR microclusters or signalosomes in the IS in a polymerized actin-dependent manner. In this way, SLP-2 helps to sustain TCR-dependent signalling and enhances T cell activation

Dust in Spiral Galaxies: Comparing Emission and Absorption to Constrain Small-Scale and Very Cold Structures

The detailed distribution of dust in the disks of spiral galaxies is important to understanding the radiative transfer within disks, and to measuring overall dust masses if significant quantities of dust are either very opaque or very cold. We address this issue by comparing measures of dust absorption, using the galaxy-overlap technique in the optical, with measures of the dust grains' thermal emission from 50-2000 micron using ISOPHOT on board ISO and SCUBA at the JCMT. We examine three spiral galaxies projected partially in front of E/S0 galaxies --- AM1316-241, NGC 5545, and NGC 5091 (for NGC 5091 we have only optical and ISO data). Adopting an empirical exponential model for the dust distribution, we compare column densities and dust masses derived from the absorption and emission techniques. This comparison is sensitive to the amount of dust mass in small, opaque structures, which would not contribute strongly to area-weighted absorption measures, and to very cold dust, which would contribute to optical absorption but provide only a small fraction of the sub-mm emission. In AM1316-241, we find global dust masses of 2-5 x 10^7 M_solar, both techniques agreeing at the 50% level. NGC 5545 has about half this dust mass. The concordance of dust masses is well within the errors expected from our knowledge of the radial distribution of dust, and argues against any dominant part of the dust mass being so cold or opaque. The 50-2000 micron data are well fitted by modified Planck functions with an emissivity law beta=-2, at 21 +/- 2 K. We also present 12 micron ISOCAM observations of these pairs.Comparison of H-alpha and 12 micron images of NGC 5545 indicate that ISOCAM images are reliable tracers of star formation.Comment: 16 pages, 4 tables, 8 figures, in press for October Astronomical Journa

EC 11481-2303 - A Peculiar Subdwarf OB Star Revisited

EC 11481-2303 is a peculiar, hot, high-gravity pre-white dwarf. Previous optical spectroscopy revealed that it is a sdOB star with an effective temperature (Teff) of 41790 K, a surface gravity log(g)= 5.84, and He/H = 0.014 by number. We present an on-going spectral analysis by means of non-LTE model-atmosphere techniques based on high-resolution, high-S/N optical (VLT-UVES) and ultraviolet (FUSE, IUE) observations. We are able to reproduce the optical and UV observations simultaneously with a chemically homogeneous NLTE model atmosphere with a significantly higher effective temperature and lower He abundance (Teff = 55000 K, log (g) = 5.8, and He / H = 0.0025 by number). While C, N, and O appear less than 0.15 times solar, the iron-group abundance is strongly enhanced by at least a factor of ten.Comment: 8 pages, 11 figure

Ordered phase in the two-dimensional randomly coupled ferromagnet

True ground states are evaluated for a 2d Ising model with random near neighbor interactions and ferromagnetic second neighbor interactions (the Randomly Coupled Ferromagnet). The spin glass stiffness exponent is positive when the absolute value of the random interaction is weaker than the ferromagnetic interaction. This result demonstrates that in this parameter domain the spin glass like ordering temperature is non-zero for these systems, in strong contrast to the 2d Edwards-Anderson spin glass.Comment: 7 pages; 9 figures; revtex; new version much extende