Manufacturing process applications team (MATeam)

Activities of the manufacturing applications team (MATeam) in effecting widespread transfer of NASA technology to aid in the solution of manufacturing problems in the industrial sector are described. During the program's first year of operation, 450 companies, industry associations, and government agencies were contacted, 150 manufacturing problems were documented, and 20 potential technology transfers were identified. Although none of the technology transfers has been commercialized and put in use, several are in the applications engineering phase, and others are in the early stages of implementation. The technology transfer process is described and guidelines used for the preparation of problems statements are included

An analysis of B cell selection mechanisms in germinal centres

Affinity maturation of antibodies during immune responses is achieved by multiple rounds of somatic hypermutation and subsequent preferential selection of those B cells that express B cell receptors with improved binding characteristics for the antigen. The mechanism underlying B cell selection has not yet been defined. By employing an agent-based model, we show that for physiologically reasonable parameter values affinity maturation can be driven by competition for neither binding sites nor antigen—even in the presence of competing secreted antibodies. Within the tested mechanisms, only clonal competition for T cell help or a refractory time for the interaction of centrocytes with follicular dendritic cells is found to enable affinity maturation while generating the experimentally observed germinal centre characteristics and tolerating large variations in the initial antigen density

Analysis of B cell selection mechanisms in the adaptive immune response

The essential task of a germinal centre reaction is the selection of those B cells that bind the antigen with high affinity. The exact mechanisms of B cell selection is still unknown and rather difficult to be accessed in experiment. With the help of an already established agent-based model for the space-time-dynamics of germinal centre reactions [1,2] we compare the most important hypotheses for what the limiting factor for B cell rescue may be. We discuss competition for antigen sites on follicular dendritic cells, a refractory time for centrocytes after every encounter with follicular dendritic cells, competition for the antigen itself, the role of antigen masking with soluble antibodies, and competition for T cell help. The unexpected result is that neither competition for interaction sites nor competition for antigen nor antigen masking are in agreement with present experimental data on germinal centre reactions. We show that these most popular selection mechanisms do not lead to sufficient affinity maturation and do not respect the observed robustness against changes of initial conditions. However, the best agreement with data was found for the newly hypothesized centrocyte refractory time and for competition for T cell help. Thus the in silico experiments point towards selection mechanisms that are not in the main focus of current germinal centre research. Possible experiments to test these hypotheses are proposed

Impact of Electrostatic Forces in Contact Mode Scanning Force Microscopy

In this $\ll$ contribution we address the question to what extent surface charges affect contact-mode scanning force microscopy measurements. % We therefore designed samples where we could generate localized electric field distributions near the surface as and when required. % We performed a series of experiments where we varied the load of the tip, the stiffness of the cantilever and the hardness of the sample surface. % It turned out that only for soft cantilevers could an electrostatic interaction between tip and surface charges be detected, irrespective of the surface properties, i.\,e. basically regardless its hardness. % We explain these results through a model based on the alteration of the tip-sample potential by the additional electric field between charged tip and surface charges

Development of dry coal feeders

Design and fabrication of equipment of feed coal into pressurized environments were investigated. Concepts were selected based on feeder system performance and economic projections. These systems include: two approaches using rotating components, a gas or steam driven ejector, and a modified standpipe feeder concept. Results of development testing of critical components, design procedures, and performance prediction techniques are reviewed

An Improbable Solution to the Underluminosity of 2M1207B: A Hot Protoplanet Collision Afterglow

We introduce an alternative hypothesis to explain the very low luminosity of the cool (L-type) companion to the ~25 M_Jup ~8 Myr-old brown dwarf 2M1207A. Recently, Mohanty et al. (2007) found that effective temperature estimates for 2M1207B (1600 +- 100 K) are grossly inconsistent with its lying on the same isochrone as the primary, being a factor of ~10 underluminous at all bands between I (0.8 um) and L' (3.6 um). Mohanty et al. explain this discrepency by suggesting that 2M1207B is an 8 M_Jup object surrounded by an edge-on disk comprised of large dust grains producing 2.5^m of achromatic extinction. We offer an alternative explanation: the apparent flux reflects the actual source luminosity. Given the temperature, we infer a small radius (~49,000 km), and for a range of plausible densities, we estimate a mass < M_Jup. We suggest that 2M1207B is a hot protoplanet collision afterglow and show that the radiative timescale for such an object is >~1% the age of the system. If our hypothesis is correct, the surface gravity of 2M1207B should be an order of magnitude lower than predicted by Mohanty et al. (2007).Comment: ApJ Letters, in press (11 pages

Binding between two-component bosons in one dimension

We investigate the ground state of one-dimensional few-atom Bose-Bose mixtures under harmonic confinement throughout the crossover from weak to strong inter-species attraction. The calculations are based on the numerically exact multi-configurational time-dependent Hartree method. For repulsive components we detail the condition for the formation of a molecular Tonks-Girardeau gas in the regime of intermediate inter-species interactions, and the formation of a molecular condensate for stronger coupling. Beyond a critical inter-species attraction, the system collapses to an overall bound state. Different pathways emerge for unequal particle numbers and intra-species interactions. In particular, for mixtures with one attractive component, this species can be viewed as an effective potential dimple in the trap center for the other, repulsive component.Comment: 10 pages, 10 figure

Drag reductions obtained by modifying a box-shaped ground vehicle

A box-shaped ground vehicle was used to simulate the aerodynamic drag of high volume transports, that is, delivery vans, trucks, or motor homes. The coast-down technique was used to define the drag of the original vehicle, having all square corners, and several modifications of the vehicle. Test velocities ranged up to 65 miles per hour, which provided maximum Reynolds numbers of 1 times 10 to the 7th power based on vehicle length. One combination of modifications produced a reduction in aerodynamic drag of 61 percent as compared with the original square-cornered vehicle

Acid-Labile Traceless Click Linker for Protein Transduction

Intracellular delivery of active proteins presents an interesting approach in research and therapy. We created a protein transduction shuttle based on a new traceless click linker that combines the advantages of click reactions with implementation of reversible pH-sensitive bonds. The azidomethyl-methylmaleic anhydride (AzMMMan) linker was found compatible with different click chemistries, demonstrated in bioreversible protein modification with dyes, polyethylene glycol, or a transduction carrier. Linkages were stable at physiological pH but reversible at the mild acidic pH of endosomes or lysosomes. We show that pH-reversible attachment of a defined endosome-destabilizing three-arm oligo(ethane amino)amide carrier generates an effective shuttle for protein delivery. The cargo protein nlsEGFP, when coupled via the traceless AzMMMan linker, experiences efficient cellular uptake and endosomal escape into the cytosol, followed by import into the nucleus. In contrast, irreversible linkage to the same shuttle hampers nuclear delivery of nlsEGFP which after uptake remains trapped in the cytosol. Successful intracellular delivery of bioactive ß-galactosidase as a model enzyme was also demonstrated using the pH-controlled shuttle system