A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain neurons.

Nanobodies (nAbs) are small, minimal antibodies that have distinct attributes that make them uniquely suited for certain biomedical research, diagnostic and therapeutic applications. Prominent uses include as intracellular antibodies or intrabodies to bind and deliver cargo to specific proteins and/or subcellular sites within cells, and as nanoscale immunolabels for enhanced tissue penetration and improved spatial imaging resolution. Here, we report the generation and validation of nAbs against a set of proteins prominently expressed at specific subcellular sites in mammalian brain neurons. We describe a novel hierarchical validation pipeline to systematically evaluate nAbs isolated by phage display for effective and specific use as intrabodies and immunolabels in mammalian cells including brain neurons. These nAbs form part of a robust toolbox for targeting proteins with distinct and highly spatially-restricted subcellular localization in mammalian brain neurons, allowing for visualization and/or modulation of structure and function at those sites

Picosecond absorption relaxation measured with nanosecond laser photoacoustics

Picosecond absorption relaxation—central to many disciplines—is typically measured by ultrafast (femtosecond or picosecond) pump-probe techniques, which however are restricted to optically thin and weakly scattering materials or require artificial sample preparation. Here, we developed a reflection-mode relaxation photoacoustic microscope based on a nanosecond laser and measured picosecond absorption relaxation times. The relaxation times of oxygenated and deoxygenated hemoglobin molecules, both possessing extremely low fluorescence quantum yields, were measured at 576 nm. The added advantages in dispersion susceptibility, laser-wavelength availability, reflection sensing, and expense foster the study of natural—including strongly scattering and nonfluorescent—materials

Residents’ Perception of Destination Quality: Key Factors for Sustainable Rural Development

Tourism represents a viable alternative for economic activities in rural areas, and improves the living standards of the communities. The aim of the paper is to assess residents’ perceptions towards tourism destination quality in the North-West Development Region of Romania using the following items: natural attractions: cultural and social attractions; accommodation; food; availability of transportation to destination area; quality of information and communication; hospitality of host community; feeling of security and safety. A survey was conducted to collect the data and a total of 433 questionnaires were validated and analyzed. Descriptive and inferential statistics (Principal component analysis, ANOVA, Scheffe multiple range test) were used to analyze the data. Results show that there are statistically significant differences in residents’ perceptions between counties. Two of the components of destination quality (general infrastructure and tourism potential) are affected by the gender of residents, while the level of education has no significant effect. A weak and indirect correlation was found between the age of residents and their perception towards a quality destination. This study makes two contributions to the existing literature. First, a questionnaire was developed based on the QUALITEST tool adapted to the realities of the research area. Second, we analyzed the perception of residents towards a quality destination in relation to their socio-demographic characteristics and county of residence. Understanding the implications of tourism development from the residents’ point of view helps to increase knowledge about the factors affecting the long-term, sustainable success of tourism destinations