11 research outputs found
Reduced mitochondrial respiration in T cells of patients with major depressive disorder
Converging evidence indicates that major depressive disorder (MDD) and metabolic disorders might be mediated by shared (patho)biological pathways. However, the converging cellular and molecular signatures remain unknown. Here, we investigated metabolic dysfunction on a systemic, cellular, and molecular level in unmedicated patients with MDD compared with matched healthy controls (HC). Despite comparable BMI scores and absence of cardiometabolic disease, patients with MDD presented with significant dyslipidemia. On a cellular level, T cells obtained from patients with MDD exhibited reduced respiratory and glycolytic capacity. Gene expression analysis revealed increased carnitine palmitoyltransferase IA (CPT1a) levels in T cells, the rate-limiting enzyme for mitochondrial long-chain fatty acid oxidation. Together, our results indicate metabolic dysfunction in unmedicated, non-overweight patients with MDD on a systemic, cellular, and molecular level. This evidence for reduced mitochondrial respiration in T cells of patients with MDD provides translation of previous animal studies regarding a putative role of altered immunometabolism in depression pathobiology
lmmunomorphologica characteristics of renal cell carcinoma
Immunomorphological characteristics of 27
renal cell carcinoma (RCC): 18 clear cell, 6 granular
(chromophilic), 2 chromophobe, 1 spindle cell
(sarcomatoid) as well as of 1 oncocytoma, were
analyzed. The investigation was performed on cryostat
sections by immunoperoxidase technique applying a
panel of monoclonal antibodies which defined: proximal
(TNE3, TN5, 5D9) and distal (TN8, TN9, 7C2) tubular
antigens; intercellular adhesion molecule 1 (ICAMl);
HLA class I1 (-DQ, -DR and -DP) antigens, intermediary
filaments (cytokeratin and vimentin); and antigens on
tumour infiltrating mononuclear leucocytes (TT1, TT2
and LeuM3 for CD4, CD8 and CD14 antigens,
respectively). All RCC with exception of chromophobe
CO-expressed cytokeratin and vimentin. In addition, they
were usually positive for all proximal and two distal
tubular markers (TN8, TN9) indicating primitive cells
which could differentiate into the epithelium of both
parts of tubule system as the most probable originators
of in RCC. Almost all RCC but the chromophobe
aberrantly expressed HLA class I1 antigens which great
variability from case to caie. The presence of HLA-DR
antigens was more intensive and widespread than of
HLA-DQ and-DP antigens. Expression of ICAMl
mostly correlated with presence of HLA class I1
antigens, particularly with -DR on tumour cells of RCC HLA-DR antigen expression was always more
prominent than mononuclear cell infiltrate (among
which macrophages prevailed over T cells) which could
suggest that increased histocompatibility antigen
expression precedes mononuclear cell influx.
In contrast to all other RCC, chromophobe tumours
had quite distinct features revealing the most intense
reaction with 7C2 (MAb that produced the weakest
reaction with other tumour types), absence of vimentin
and very weak reaction with antibodies for HLA class Il
Ag and ICAM 1. Since oncocytoma has similar immunohistological properties it could be supposed that both
tumours have common histogenesis