Improvement of surface texture on the hot dip galvanized and galvannealed steel sheets

Customers of hot dip galvanized and/or galvannealed steel sheets for automotive are more and moredemanding on high surface quality, paintability and press-formability. The roll texture for temper-rolling incontinuous galvanizing line should be very precisely treated to give a special roughness, which can betransferred to the Zn-coating surfaces during temper-rolling. Among the various texturing methods, in thepresent study, we adopted a newly developed TCT (TopoCrom Texturing) technology and both roll roughness(high/low Ra) and Cr structures (Open/Closed types) are tested. The products applying TCT had a uniformand dense roughness pattern and surface characteristics is also compared with the conventionally used EDT(electron discharge texturing) treatment. It should be noted that TCT technology on commercial galvannealingcoating surface is firstly used and the best condition was Ra=1.2 ?m with closed type; the roughness valuedecreases about 35% compared to the conventionally used EDT. On the other hand, in hot-dip galvanized steelsheets, the best condition was Ra=3.0 ?m with closed type. The friction coefficient was significantly improvedby the effect of the formation of oil pockets on the hot-dip galvanized steel sheet

Secondary Breast Augmentation: Managing Each Case

Breast augmentation is one of the most regularly performed interventions requiring reoperation in aesthetic surgery. For this reason, it involves a greater chance for complications. In this report, the authors aim to provide young plastic surgeons with guidelines based on their experience for responding to each of these complications, to explain the causes and ways of avoiding them, and to show how they can be treated when they occur

High resolution imaging reveals heterogeneity in chromatin states between cells that is not inherited through cell division

BACKGROUND: Genomes of eukaryotes exist as chromatin, and it is known that different chromatin states can influence gene regulation. Chromatin is not a static structure, but is known to be dynamic and vary between cells. In order to monitor the organisation of chromatin in live cells we have engineered fluorescent fusion proteins which recognize specific operator sequences to tag pairs of syntenic gene loci. The separation of these loci was then tracked in three dimensions over time using fluorescence microscopy. RESULTS: We established a work flow for measuring the distance between two fluorescently tagged, syntenic gene loci with a mean measurement error of 63 nm. In general, physical separation was observed to increase with increasing genomic separations. However, the extent to which chromatin is compressed varies for different genomic regions. No correlation was observed between compaction and the distribution of chromatin markers from genomic datasets or with contacts identified using capture based approaches. Variation in spatial separation was also observed within cells over time and between cells. Differences in the conformation of individual loci can persist for minutes in individual cells. Separation of reporter loci was found to be similar in related and unrelated daughter cell pairs. CONCLUSIONS: The directly observed physical separation of reporter loci in live cells is highly dynamic both over time and from cell to cell. However, consistent differences in separation are observed over some chromosomal regions that do not correlate with factors known to influence chromatin states. We conclude that as yet unidentified parameters influence chromatin configuration. We also find that while heterogeneity in chromatin states can be maintained for minutes between cells, it is not inherited through cell division. This may contribute to cell-to-cell transcriptional heterogeneity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12860-016-0111-y) contains supplementary material, which is available to authorized users

Mesenchymal stem cells: from experiment to clinic

There is currently much interest in adult mesenchymal stem cells (MSCs) and their ability to differentiate into other cell types, and to partake in the anatomy and physiology of remote organs. It is now clear these cells may be purified from several organs in the body besides bone marrow. MSCs take part in wound healing by contributing to myofibroblast and possibly fibroblast populations, and may be involved in epithelial tissue regeneration in certain organs, although this remains more controversial. In this review, we examine the ability of MSCs to modulate liver, kidney, heart and intestinal repair, and we update their opposing qualities of being less immunogenic and therefore tolerated in a transplant situation, yet being able to contribute to xenograft models of human tumour formation in other contexts. However, such observations have not been replicated in the clinic. Recent studies showing the clinical safety of MSC in several pathologies are discussed. The possible opposing powers of MSC need careful understanding and control if their clinical potential is to be realised with long-term safety for patients

Design of Respiration Rate Meter Using Flexible Sensor

Respiration rate is an important physiological parameter that helps to provide important information about the patient's health status, especially from the human respiratory system. So it is necessary to measure the human respiratory rate by calculating the number of respiratory frequencies within 1 minute. The respiratory rate meter is a tool used to calculate the respiratory rate by counting the number of breaths for 1 minute. The author makes a tool to detect human respiratory rate by using a sensor that detects the ascend and descend of the chest cavity based on a microcontroller so that the operator can measure the breathing rate more practically and accurately. Component tool contains analog signal conditioning circuit and microcontroller circuit accompanied by display in the form of LCD TFT. The results of measurement data on 10 respondents obtained an average error value, namely the position of the right chest cavity 6.6%, middle chest cavity 7.92%, and left chest cavity 6.85%. This value is still below the error tolerance limit of 10%. It can be concluded that to obtain the best measurement results, the sensor is placed in the position of the right chest cavity