Tunable far infrared laser spectrometers

The state of the art in far infrared (FIR) spectroscopy is reviewed. The development of tunable, coherent FIR radiation sources is discussed. Applications of tunable FIR laser spectrometers for measurement of rotational spectra and dipole moments of molecular ions and free radicals, vibration-rotation-tunneling (VRT) spectra of weakly bound complexes, and vibration-rotation spectra of linear carbon clusters are presented. A detailed description of the Berkeley tunable FIR laser spectrometers is presented in the following article

The Berkeley tunable far infrared laser spectrometers

A detailed description is presented for a tunable far infrared laser spectrometer based on frequency mixing of an optically pumped molecular gas laser with tunable microwave radiation in a Schottky point contact diode. The system has been operated on over 30 laser lines in the range 10–100 cm^–1 and exhibits a maximum absorption sensitivity near one part in 10^6. Each laser line can be tuned by ±110 GHz with first-order sidebands. Applications of this instrument are detailed in the preceding paper

Effect of heat treatment and aging on the mechanical loss and strength of hydroxide catalysis bonds between fused silica samples

Hydroxide catalysis bonds are used in the aLIGO gravitational wave detectors and are an essential technology within the mirror suspensions which allowed for detector sensitivities to be reached that enabled the first direct detections of gravitational waves. Methods aimed at further improving hydroxide catalysis bonds for future upgrades to these detectors, in order to increase detection rates and the number of detectable sources, are explored. Also, the effect on the bonds of an aLIGO suspension construction procedure involving heat, the fibre welding process, is investigated. Here we show that thermal treatments can be beneficial to improving some of the bond properties important to the mirror suspensions in interferometric gravitational wave detectors. It was found that heat treating bonds at 150\,^\circC increases bond strength by a factor of approximately 1.5 and a combination of bond ageing and heat treatment of the optics at 150\,\circC reduces the mechanical loss of a bond from 0.10 to 0.05. It is also shown that current construction procedures do not reduce bond strength

Thermal noise in interferometric gravitational wave detectors due to dielectric optical coatings

We report on thermal noise from the internal friction of dielectric coatings made from alternating layers of Ta2O5 and SiO2 deposited on fused silica substrates. We present calculations of the thermal noise in gravitational wave interferometers due to optical coatings, when the material properties of the coating are different from those of the substrate and the mechanical loss angle in the coating is anisotropic. The loss angle in the coatings for strains parallel to the substrate surface was determined from ringdown experiments. We measured the mechanical quality factor of three fused silica samples with coatings deposited on them. The loss angle of the coating material for strains parallel to the coated surface was found to be (4.2 +- 0.3)*10^(-4) for coatings deposited on commercially polished slides and (1.0 +- 0.3)*10^{-4} for a coating deposited on a superpolished disk. Using these numbers, we estimate the effect of coatings on thermal noise in the initial LIGO and advanced LIGO interferometers. We also find that the corresponding prediction for thermal noise in the 40 m LIGO prototype at Caltech is consistent with the noise data. These results are complemented by results for a different type of coating, presented in a companion paper.Comment: Submitted to LSC (internal) review Sept. 20, 2001. To be submitted to Phys. Lett.

Investigation of mechanical losses of thin silicon flexures at low temperatures

The investigation of the mechanical loss of different silicon flexures in a temperature region from 5 to 300 K is presented. The flexures have been prepared by different fabrication techniques. A lowest mechanical loss of $3\times10^{-8}$ was observed for a 130 $\mu$m thick flexure at around 10 K. While the mechanical loss follows the thermoelastic predictions down to 50 K a difference can be observed at lower temperatures for different surface treatments. This surface loss will be limiting for all applications using silicon based oscillators at low temperatures. The extraction of a surface loss parameter using different results from our measurements and other references is presented. We focused on structures that are relevant for gravitational wave detectors. The surface loss parameter $\alpha_s$ = 0.5 pm was obtained. This reveals that the surface loss of silicon is significantly lower than the surface loss of fused silica.Comment: 16 pages, 7 figure

A Characterization of Scale Invariant Responses in Enzymatic Networks

An ubiquitous property of biological sensory systems is adaptation: a step increase in stimulus triggers an initial change in a biochemical or physiological response, followed by a more gradual relaxation toward a basal, pre-stimulus level. Adaptation helps maintain essential variables within acceptable bounds and allows organisms to readjust themselves to an optimum and non-saturating sensitivity range when faced with a prolonged change in their environment. Recently, it was shown theoretically and experimentally that many adapting systems, both at the organism and single-cell level, enjoy a remarkable additional feature: scale invariance, meaning that the initial, transient behavior remains (approximately) the same even when the background signal level is scaled. In this work, we set out to investigate under what conditions a broadly used model of biochemical enzymatic networks will exhibit scale-invariant behavior. An exhaustive computational study led us to discover a new property of surprising simplicity and generality, uniform linearizations with fast output (ULFO), whose validity we show is both necessary and sufficient for scale invariance of enzymatic networks. Based on this study, we go on to develop a mathematical explanation of how ULFO results in scale invariance. Our work provides a surprisingly consistent, simple, and general framework for understanding this phenomenon, and results in concrete experimental predictions

Secondary somatic mutations restoring RAD51C and RAD51D associated with acquired resistance to the PARP inhibitor rucaparib in high-grade ovarian carcinoma

High-grade epithelial ovarian carcinomas (OC) containing mutated BRCA1 or BRCA2 (BRCA1/2) homologous recombination (HR) genes are sensitive to platinum-based chemotherapy and poly(ADP-ribose) polymerase inhibitors (PARPi), while restoration of HR function due to secondary mutations in BRCA1/2 has been recognized as an important resistance mechanism. We sequenced core HR pathway genes in 12 pairs of pre-treatment and post-progression tumor biopsy samples collected from patients in ARIEL2 Part 1, a phase 2 study of the PARPi rucaparib as treatment for platinum-sensitive, relapsed OC. In six of 12 pre-treatment biopsies, a truncation mutation in BRCA1, RAD51C or RAD51D was identified. In five of six paired post-progression biopsies, one or more secondary mutations restored the open reading frame. Four distinct secondary mutations and spatial heterogeneity were observed for RAD51C. In vitro complementation assays and a patient-derived xenograft (PDX), as well as predictive molecular modeling, confirmed that resistance to rucaparib was associated with secondary mutations