On the phagocytosis in vitro of strains of Yersinia pseudotuberculosis after treatment with certain detergents and lipid solvents

Reproduced from SciFinder with permission from the Chemical Abstracts Service. The addn. of Na lauryl sulfate [151-21-3] or petroleum ether to Y. pseudotuberculosis grown in a medium contg. 10% glycerol at 28° (conditions under which V and W antigens are not synthesized) significantly increased the phagocytosis of the bacterial cells by rabbit blood. The addn. of Na periodate or trypsin [9002-07-7] produced variable degrees of phagocytosis dependent on the virulence of the strains tested. The role of lipid synthesis in the restoration of phagocytosis at 28° is discussed

Further studies on the metabolic changes in strains of Salmonella typhimurium and Yersinia pseudotuberculosis after treatment with some detergents and lipid solvents

In Bulgarian.Reproduced from SciFinder, with permission from the Chemical Abstracts Service. Incubation of S. typhimurium and Y. pseudotuberculosis in 30% petroleum ether at 37° inhibited respiration more than did 0.01-0.03% Na lauryl sulfate [151-21-3]. After a 72-h incubation in 10% glycerol [56-81-5] the respiration level returned to, or approached normal. Petroleum ether also inhibited dehydrogenase [9035-82-9] activity and glycolysis more than did Na lauryl sulfate. Incubation in glycerol also reversed this inhibition. Na lauryl sulfate completely and irreversibly inhibited phosphatase [9013-05-2] at pH 5.4 and 8.0. Petroleum ether inhibited phosphatase at pH 5.4 in Y. pseudotuberculosis and at pH 8.0 in S. typhimurium

Some biological properties of bacterial lipids

Review with 44 references. In Bulgaria

Studies on the ultrastructure of Yersinia pseudotuberculosis after treatment with some detergents and solvents

The ultrastructural changes in 3 strains Yersinia pseudotuberculosis with different virulence after treatment with sodium lauryl sulfate (SLS) and petroleum ether were studied. The ultrafine sections after treatment with SLS show heavy destructive changes, concerning the cell wall, the cytoplasmic membrane and the inner structure of the cell. It was established that the same cells Y. pseudotuberculosis after cultivation on a medium with glycerol show a tendency to recover their ultrastructure. The cells treated with petroleum ether did not exhibit any notable ultrastructural changes

Experimental kinetics of infection induced by Yersinia pseudotuberculosis isolated from stock animals

The course of in vivo infection of five isolates of Yersinia pseudotuberculosis was followed for three weeks in Swiss mice. The strains were isolated from diarrheic and normal feces and mesenteric lymph nodes of healthy and sick stock animals. Four strains of serogroup O:3 and one of serogroup O:1a, with and without the virulence plasmid, were inoculated intragastrically and intravenously in the mice. Groups of five animals were sacrificed at 6 h and 3, 6, 10, 15, and 21 days after inoculation, and organs and tissues were checked for possible macroscopic alterations. Development of infection was monitored at these times by performing viable bacterial counts in homogenates of selected tissues. The animals were cheked daily for clinical alterations. The results of the study showed that strains with the virulence plasmid infected organs and tissues at various times and at varying intensity by both routes of infection, the strain of type O:1a being the most invasive. Moreover, clinical and pathological alterations occurred only in animals inoculated with bacteria carrying the virulence plasmid, regardless of the route of infection