Expression of Recombinant Fatty Acid Binding Protein from <i>Fasciola gigantica</i>

549-552Fatty acid binding protein (FADP) is an attractive vaccine candidate and a drug target for controlling fasciolosis caused by Fasciola gigantica in ruminants. The cDNA encoding F. gigantica FADP was amplified by polymerase chain reaction and expressed in Escherichia coli as a recombinant protein using a prokaryotic expression system. The protein was purified to homogeneity by nickel chelating affinity chromatography and subsequently by ion-exchange chromatography. The recombinant protein thus obtained will be assessed for immuno-prophylaxis and for its immunodiagnostic potential in ruminants agaiust F. gigantica.</i

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

On the Indian sub-continent, nearly 5,000 years ago, the domestication of the riverine buffalo—the incredible Asian dairy animal was initiated. It plays a versatile role in socio-economic upliftment of its owners from the rural agricultural communities in Asian, African, South American and a few European countries. Comparatively, buffaloes are lesser evolved and susceptible to infectious diseases than cattle. However, poor body thermoregulation and wallowing nature predisposed them to snail borne infections, especially tropical fasciolosis—an incessant major constraint on buffalo production and improvement programmes. This review article is an insight into the global prevalence, varied epizootiological factors, offers possible explanation to pathophysiological clinical signs, deleterious effects of the tropical liver fluke, involving hepato-biliary system, haemopoitic system, endocrine glands and their secretions, oxidative stress, altered metabolism and significant fall in food conversion efficiency with unaffected digestibility of nutrients. Besides, the authors have briefly discussed and reviewed the developments and significance of successful immunodiagnostic approaches for detecting and forecasting the disease during early pre-patency and feasibility of developing a cost effective immunoprotection strategies against tropical fasciolosis