Pesticide Exposure Alters Follicle-Stimulating Hormone Levels in Mexican Agricultural Workers

Organophosphorous pesticides (OPs) are suspected of altering reproductive function by reducing brain acetylcholinesterase activity and monoamine levels, thus impairing hypothalamic and/or pituitary endocrine functions and gonadal processes. Our objective was to evaluate in a longitudinal study the association between OP exposure and serum levels of pituitary and sex hormones. Urinary OP metabolite levels were measured by gas–liquid chromatography, and serum pituitary and sex hormone levels by enzymatic immunoassay and radioimmunoassay in 64 men. A total of 147 urine and blood samples were analyzed for each parameter. More than 80% of the participants had at least one OP metabolite in their urine samples. The most frequent metabolite found was diethylthiophosphate (DETP; 55%), followed by diethylphosphate (DEP; 46%), dimethylthiophosphate (DMTP; 32%), and dimethyldithiophosphate (DMDTP; 31%). However, the metabolites detected at higher concentrations were DMTP, DEP, DMDTP, and dimethylphosphate. There was a high proportion of individuals with follicle-stimulating hormone (FSH) concentrations outside the range of normality (48%). The average FSH serum levels were higher during the heavy pesticide spraying season. However, a multivariate analysis of data collected in all periods showed that serum FSH levels were negatively associated with urinary concentrations of both DMTP and DMDTP, whereas luteinizing hormone (LH) was negatively associated with DMTP. We observed no significant associations between estradiol or testosterone serum levels with OP metabolites. The hormonal disruption in agricultural workers presented here, together with results from experimental animal studies, suggests that OP exposure disrupts the hypothalamic–pituitary endocrine function and also indicates that FSH and LH are the hormones most affected

Reactions of a Be-10 beam on proton and deuteron targets

The extraction of detailed nuclear structure information from transfer reactions requires reliable, well-normalized data as well as optical potentials and a theoretical framework demonstrated to work well in the relevant mass and beam energy ranges. It is rare that the theoretical ingredients can be tested well for exotic nuclei owing to the paucity of data. The halo nucleus Be-11 has been examined through the 10Be(d,p) reaction in inverse kinematics at equivalent deuteron energies of 12,15,18, and 21.4 MeV. Elastic scattering of Be-10 on protons was used to select optical potentials for the analysis of the transfer data. Additionally, data from the elastic and inelastic scattering of Be-10 on deuterons was used to fit optical potentials at the four measured energies. Transfers to the two bound states and the first resonance in Be-11 were analyzed using the Finite Range ADiabatic Wave Approximation (FR-ADWA). Consistent values of the spectroscopic factor of both the ground and first excited states were extracted from the four measurements, with average values of 0.71(5) and 0.62(4) respectively. The calculations for transfer to the first resonance were found to be sensitive to the size of the energy bin used and therefore could not be used to extract a spectroscopic factor.Comment: 16 Pages, 10 figure

Developmental Acquisition of a Rapid Calcium-Regulated Vesicle Supply Allows Sustained High Rates of Exocytosis in Auditory Hair Cells

Auditory hair cells (HCs) have the remarkable property to indefinitely sustain high rates of synaptic vesicle release during ongoing sound stimulation. The mechanisms of vesicle supply that allow such indefatigable exocytosis at the ribbon active zone remain largely unknown. To address this issue, we characterized the kinetics of vesicle recruitment and release in developing chick auditory HCs. Experiments were done using the intact chick basilar papilla from E10 (embryonic day 10) to P2 (two days post-hatch) by monitoring changes in membrane capacitance and Ca2+ currents during various voltage stimulations. Compared to immature pre-hearing HCs (E10-E12), mature post-hearing HCs (E18-P2) can steadily mobilize a larger readily releasable pool (RRP) of vesicles with faster kinetics and higher Ca2+ efficiency. As assessed by varying the inter-pulse interval of a 100 ms paired-pulse depolarization protocol, the kinetics of RRP replenishment were found much faster in mature HCs. Unlike mature HCs, exocytosis in immature HCs showed large depression during repetitive stimulations. Remarkably, when the intracellular concentration of EGTA was raised from 0.5 to 2 mM, the paired-pulse depression level remained unchanged in immature HCs but was drastically increased in mature HCs, indicating that the Ca2+ sensitivity of the vesicle replenishment process increases during maturation. Concomitantly, the immunoreactivity of the calcium sensor otoferlin and the number of ribbons at the HC plasma membrane largely increased, reaching a maximum level at E18-P2. Our results suggest that the efficient Ca2+-dependent vesicle release and supply in mature HCs essentially rely on the concomitant engagement of synaptic ribbons and otoferlin at the plasma membrane

Direct neutron capture cross section on Ge 80 and probing shape coexistence in neutron-rich nuclei

Results are presented from the first neutron-transfer measurement on Ge80 using an exotic beam from the Holifield Radioactive Ion Beam Facility at Oak Ridge National Laboratory. Newly measured spins and spectroscopic factors of low-lying states of Ge81 are determined, and the neutron capture cross section on Ge80 was calculated in a direct-semidirect model to provide a more realistic (n,γ) reaction rate for r-process simulations. Furthermore, a region of shape coexistence around N≈50 is confirmed and implications for the magic nature of Ni78 are discussed

Halo nucleus be11: A spectroscopic study via neutron transfer

The best examples of halo nuclei, exotic systems with a diffuse nuclear cloud surrounding a tightly bound core, are found in the light, neutron-rich region, where the halo neutrons experience only weak binding and a weak, or no, potential barrier. Modern direct-reaction measurement techniques provide powerful probes of the structure of exotic nuclei. Despite more than four decades of these studies on the benchmark one-neutron halo nucleus Be11, the spectroscopic factors for the two bound states remain poorly constrained. In the present work, the Be10(d,p) reaction has been used in inverse kinematics at four beam energies to study the structure of Be11. The spectroscopic factors extracted using the adiabatic model were found to be consistent across the four measurements and were largely insensitive to the optical potential used. The extracted spectroscopic factor for a neutron in an nâ., j=2s 1/2 state coupled to the ground state of Be10 is 0.71(5). For the first excited state at 0.32Â MeV, a spectroscopic factor of 0.62(4) is found for the halo neutron in a 1p 1/2 state. © 2012 American Physical Society

Bimodality and Gaps on Globular Cluster Horizontal Branches. II. The Cases of NGC 6229, NGC 1851 and NGC 2808

The outer-halo globular cluster NGC 6229 has a peculiar horizontal-branch (HB) morphology, with clear indications of a bimodal HB and a ``gap" on the blue HB. In this paper, we present extensive synthetic HB simulations to determine whether peculiar distributions in the underlying physical parameters are needed to explain the observed HB morphology. We find that a unimodal mass distribution along the HB can satisfactorily account for the observed HB bimodality, *provided* the mass dispersion is substantially larger than usually inferred for the Galactic globular clusters. In this case, NGC 6229 should have a well-populated, extended blue tail. A truly bimodal distribution in HB masses can also satisfactorily account for the observed HB morphology, although in this case the existence of an extended blue tail is not necessarily implied. The other two well-known bimodal-HB clusters, NGC 1851 and NGC 2808, are briefly analyzed. While the HB morphology of NGC 1851 can also be reproduced with a unimodal mass distribution assuming a large mass dispersion, the same is not true of NGC 2808, for which a bimodal, and possibly multimodal, mass distribution seems definitely required. The problem of gaps on the blue HB is also discussed. Applying the standard Hawarden (1971) and Newell (1973) chi-squared test, we find that the NGC 6229 gap is significant at the 99.7% level. However, in a set of 1,000 simulations, blue-HB gaps comparable to the observed one are present in ~ 6 - 9% of all cases. We employ a new and simple formalism, based on the binomial distribution, to explain the origin of this discrepancy, and conclude that Hawarden's method, in general, substantially overestimates the statistical significance of gaps.Comment: 50 pages (includes 5 tables and 18 multi-panel figures). Higher-resolution versions of Figs. 15a and 15b are available from the first author upon request. To appear in The Astrophysical Journa

Structure and Function of the Hair Cell Ribbon Synapse

Faithful information transfer at the hair cell afferent synapse requires synaptic transmission to be both reliable and temporally precise. The release of neurotransmitter must exhibit both rapid on and off kinetics to accurately follow acoustic stimuli with a periodicity of 1 ms or less. To ensure such remarkable temporal fidelity, the cochlear hair cell afferent synapse undoubtedly relies on unique cellular and molecular specializations. While the electron microscopy hallmark of the hair cell afferent synapse — the electron-dense synaptic ribbon or synaptic body — has been recognized for decades, dissection of the synapse’s molecular make-up has only just begun. Recent cell physiology studies have added important insights into the synaptic mechanisms underlying fidelity and reliability of sound coding. The presence of the synaptic ribbon links afferent synapses of cochlear and vestibular hair cells to photoreceptors and bipolar neurons of the retina. This review focuses on major advances in understanding the hair cell afferent synapse molecular anatomy and function that have been achieved during the past years