Using biomarkers in an evolutionary context: Lessons from the analysis of biological responses of oligochaete annelids to metal exposure

International audienceAnthropogenic activities may lead to the accumulation of inorganic and organic compounds in topsoils. Biota living in close contact with contaminated soils may experience stress at different levels of biological organization throughout the continuum from molecular to community level. Biological responses observed at the individual or infra-individual level of biological organization led to the development of biomarkers. The development of biomarkers consists often in evidencing biological modifications following a contaminant stress in laboratory conditions, using naïve organisms and it is sometime proposed to use the biological state of individuals from sentinel species collected in the field to evaluate the level of environmental exposure. However, considering the possibility of local adaptation following long-term exposure, organisms response sampled in the field may substantially differ from laboratory specimens. In this review, we discuss this point focusing on the definition and validity of molecular biomarkers of metal pollution using earthworms of the Lumbricidae family. © 2013 Elsevier Ltd. All rights reserved

Isolation and characterization of eight polymorphic microsatellites markers for the earthworm Lumbricus terrestris

International audienc

Evolutionary origin of Zinc and Cadmium tolerance in Arabidopsis halleri: Identification of Quantitative Trait Loci in metallicolous and non-metallicolous accessions

info:eu-repo/semantics/publishe

Evolutionary origin of zinc and cadmium tolerance in Arabidopsis halleri :identification of major Quantitative Trait Loci in a non-metallicolous accession

info:eu-repo/semantics/publishe

Potential preadaptation to anthropogenic pollution: evidence from a common quantitative trait locus for zinc and cadmium tolerance in metallicolous and nonmetallicolous accessions of Arabidopsis halleri

As a drastic environmental change, metal pollution may promote the rapid evolution of genetic adaptations contributing to metal tolerance. In Arabidopsis halleri, genetic bases of zinc (Zn) and cadmium (Cd) tolerance have been uncovered only in a metallicolous accession, although tolerance is species-wide. The genetic determinants of Zn and Cd tolerance in a nonmetallicolous accession were thus investigated for the first time. The genetic architecture of tolerance was investigated in a nonmetallicolous population (SK2) by using first backcross progeny obtained from crosses between SK2 and Arabidopsis lyrata petraea, a nonmetallophyte species. Only one significant and common quantitative trait locus (QTL) region was identified explaining 22.6% and 31.2% of the phenotypic variation for Zn and Cd tolerance, respectively. This QTL co-localized with HEAVY METAL ATPASE 4 (AhHMA4), which was previously validated as a determinant of Zn and Cd tolerance in a metallicolous accession. Triplication and high expression of HMA4 were confirmed in SK2. In contrast, gene duplication and high expression of METAL TOLERANT PROTEIN 1A (MTP1A), which was previously associated with Zn tolerance in a metallicolous accession, were not observed in SK2. Overall, the results support the role of HMA4 in tolerance capacities of A. halleri that may have pre-existed in nonmetallicolous populations before colonization of metal-polluted habitats. Preadaptation to metal-contaminated sites is thus discussed.SCOPUS: ar.jFLWINinfo:eu-repo/semantics/publishe