21 research outputs found
Kinetic effects of extracellular calcium on the potassium outward rectifier in the plasma membrane of Saccharomyces cerevisiae.
Kinetic effects of extracellular calcium on the potassium outward rectifier in the plasma membrane ofSaccharomyces cerevisiae
In-situ and laboratory measurements of coal matrix and cleat permeability
Micropores and the cleat network of coal provide the principal source of permeability for fluid flow. The flow behavior of fluids under varying total stress and different environmental conditions are examined in field and laboratory experiments. Based on the measurements a multi-scale, two-phase flow model to describe and quantify the various effects of the mine operation will be developed with a focus on the challenge of extrapolating point based results from a small scale to a regional scale
The S. cerevisiae outwardly-rectifying potassium channel (DUK1) identifies a new family of channels with duplicated pore domains.
Potassium channel subunits have six or two transmembrane segments in addition to a conserved pore-forming (P) domain; four subunits come together to form a channel. A gene was identified in S. cerevisiae (J0911) encoding a protein with eight probable membrane-spanning segments and two such P regions. This protein (Duk1p) is a potassium channel because Xenopus oocytes injected with the corresponding RNA express potassium currents activated by depolarization that are not seen in control oocytes. Similar potassium currents were recorded from wildtype S. cerevisiae spheroplasts, but not from those in which the DUK1 locus had been disrupted. Cells carrying the duk1 delta 1::HIS disruption in addition to a chimeric gene comprising DUK1 behind the GAL1 promoter showed outward currents when grown in galactose, but not when grown in glucose. Additional sequences with the duplicate pore motif were found in C. elegans, suggesting that these proteins represent a novel structural family of potassium channel proteins
Hochschule der Bundeswehr Muenchen, Institut fuer Wasserwesen. Mitteilungen
With 5 contributionsSIGLETIB Hannover: RN 6966(14) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman