108 research outputs found
Visualizing sound emission of elephant vocalizations: evidence for two rumble production types
Recent comparative data reveal that formant frequencies are cues to body size in animals, due to a close relationship between formant frequency spacing, vocal tract length and overall body size. Accordingly, intriguing morphological adaptations to elongate the vocal tract in order to lower formants occur in several species, with the size exaggeration hypothesis being proposed to justify most of these observations. While the elephant trunk is strongly implicated to account for the low formants of elephant rumbles, it is unknown whether elephants emit these vocalizations exclusively through the trunk, or whether the mouth is also involved in rumble production. In this study we used a sound visualization method (an acoustic camera) to record rumbles of five captive African elephants during spatial separation and subsequent bonding situations. Our results showed that the female elephants in our analysis produced two distinct types of rumble vocalizations based on vocal path differences: a nasally- and an orally-emitted rumble. Interestingly, nasal rumbles predominated during contact calling, whereas oral rumbles were mainly produced in bonding situations. In addition, nasal and oral rumbles varied considerably in their acoustic structure. In particular, the values of the first two formants reflected the estimated lengths of the vocal paths, corresponding to a vocal tract length of around 2 meters for nasal, and around 0.7 meters for oral rumbles. These results suggest that African elephants may be switching vocal paths to actively vary vocal tract length (with considerable variation in formants) according to context, and call for further research investigating the function of formant modulation in elephant vocalizations. Furthermore, by confirming the use of the elephant trunk in long distance rumble production, our findings provide an explanation for the extremely low formants in these calls, and may also indicate that formant lowering functions to increase call propagation distances in this species'
Do Zebra Finch Parents Fail to Recognise Their Own Offspring?
Individual recognition systems require the sender to be individually distinctive and the receiver to be able to perceive differences between individuals and react accordingly. Many studies have demonstrated that acoustic signals of almost any species contain individualized information. However, fewer studies have tested experimentally if those signals are used for individual recognition by potential receivers. While laboratory studies using zebra finches have shown that fledglings recognize their parents by their “distance call”, mutual recognition using the same call type has not been demonstrated yet. In a laboratory study with zebra finches, we first quantified between-individual acoustic variation in distance calls of fledglings. In a second step, we tested recognition of fledgling calls by parents using playback experiments. With a discriminant function analysis, we show that individuals are highly distinctive and most measured parameters show very high potential to encode for individuality. The response pattern of zebra finch parents shows that they do react to calls of fledglings, however they do not distinguish between own and unfamiliar offspring, despite individual distinctiveness. This finding is interesting in light of the observation of a high percentage of misdirected feedings in our communal breeding aviaries. Our results demonstrate the importance of adopting a receiver's perspective and suggest that variation in fledgling contact calls might not be used in individual recognition of offspring
HLA-DP on Epithelial Cells Enables Tissue Damage by NKp44<sup>+</sup> Natural Killer Cells in Ulcerative Colitis
Background & Aims: Ulcerative colitis (UC) is characterized by severe inflammation and destruction of the intestinal epithelium, and is associated with specific risk single nucleotide polymorphisms in HLA class II. Given the recently discovered interactions between subsets of HLA-DP molecules and the activating natural killer (NK) cell receptor NKp44, genetic associations of UC and HLA-DP haplotypes and their functional implications were investigated. Methods: HLA-DP haplotype and UC risk association analyses were performed (UC: n = 13,927; control: n = 26,764). Expression levels of HLA-DP on intestinal epithelial cells (IECs) in individuals with and without UC were quantified. Human intestinal 3-dimensional (3D) organoid cocultures with human NK cells were used to determine functional consequences of interactions between HLA-DP and NKp44. Results: These studies identified HLA-DPA1∗01:03-DPB1∗04:01 (HLA-DP401) as a risk haplotype and HLA-DPA1∗01:03-DPB1∗03:01 (HLA-DP301) as a protective haplotype for UC in European populations. HLA-DP expression was significantly higher on IECs of individuals with UC compared with controls. IECs in human intestinal 3D organoids derived from HLA-DP401pos individuals showed significantly stronger binding of NKp44 compared with HLA-DP301pos IECs. HLA-DP401pos IECs in organoids triggered increased degranulation and tumor necrosis factor production by NKp44+ NK cells in cocultures, resulting in enhanced epithelial cell death compared with HLA-DP301pos organoids. Blocking of HLA-DP401–NKp44 interactions (anti-NKp44) abrogated NK cell activity in cocultures. Conclusions: We identified an UC risk HLA-DP haplotype that engages NKp44 and activates NKp44+ NK cells, mediating damage to intestinal epithelial cells in an HLA-DP haplotype–dependent manner. The molecular interaction between NKp44 and HLA-DP401 in UC can be targeted by therapeutic interventions to reduce NKp44+ NK cell–mediated destruction of the intestinal epithelium in UC.</p
HLA-DP on Epithelial Cells Enables Tissue Damage by NKp44<sup>+</sup> Natural Killer Cells in Ulcerative Colitis
Background & Aims: Ulcerative colitis (UC) is characterized by severe inflammation and destruction of the intestinal epithelium, and is associated with specific risk single nucleotide polymorphisms in HLA class II. Given the recently discovered interactions between subsets of HLA-DP molecules and the activating natural killer (NK) cell receptor NKp44, genetic associations of UC and HLA-DP haplotypes and their functional implications were investigated. Methods: HLA-DP haplotype and UC risk association analyses were performed (UC: n = 13,927; control: n = 26,764). Expression levels of HLA-DP on intestinal epithelial cells (IECs) in individuals with and without UC were quantified. Human intestinal 3-dimensional (3D) organoid cocultures with human NK cells were used to determine functional consequences of interactions between HLA-DP and NKp44. Results: These studies identified HLA-DPA1∗01:03-DPB1∗04:01 (HLA-DP401) as a risk haplotype and HLA-DPA1∗01:03-DPB1∗03:01 (HLA-DP301) as a protective haplotype for UC in European populations. HLA-DP expression was significantly higher on IECs of individuals with UC compared with controls. IECs in human intestinal 3D organoids derived from HLA-DP401pos individuals showed significantly stronger binding of NKp44 compared with HLA-DP301pos IECs. HLA-DP401pos IECs in organoids triggered increased degranulation and tumor necrosis factor production by NKp44+ NK cells in cocultures, resulting in enhanced epithelial cell death compared with HLA-DP301pos organoids. Blocking of HLA-DP401–NKp44 interactions (anti-NKp44) abrogated NK cell activity in cocultures. Conclusions: We identified an UC risk HLA-DP haplotype that engages NKp44 and activates NKp44+ NK cells, mediating damage to intestinal epithelial cells in an HLA-DP haplotype–dependent manner. The molecular interaction between NKp44 and HLA-DP401 in UC can be targeted by therapeutic interventions to reduce NKp44+ NK cell–mediated destruction of the intestinal epithelium in UC.</p
Implications of Heavy Chain Disease Protein Sequences for Multiple Gene Theories of Immunoglobulin Synthesis
Residual stress measurement by neutron diffraction in a single bead on plate weld Influence of instrument and measurement settings on the scatter of the results
Residual stress measurements on a single bead weld on a steel plate had been performed at the High Flux Reactor of the Joint Research Centre in 2003. For these measurements a relatively old diffractometer had been used. The results obtained were characterized by significant scatter of the data, and the measurement quality suffered from the short movement ranges of the specimen positioning table as well. In 2008, a second, nominally identical, specimen from the same activity was investigated on a second diffractometer, which allowed the repetition of the residual stress measurements using different measurement settings. The present paper compares the old and the new measurement results, with a view to assessing the impact of the instrumental settings on their quality. It has been found that the overall stress distributions from the 2003 and the 2008 measurements were in fact very similar. Nevertheless, the new settings used, such as in situ specimen rocking, extended measurement duration, increased density of measurement positions and spatial resolution among others, have been found to have considerable impact on the real and on the apparent scatter of the experimental results showing that the presented methodologies can be used for improvement ofmneutron diffraction measurement
Evaluation of novel post weld heat treatment in ferritic steel repair welds based on neutron diffraction
The occurrence of cracks in normally welded components with safety relevance in, e.g. nuclear installations or in the petro chemical industry, is not an unusual event. In most cases such cracking is detected in periodic inspections prior to complete failure of the component. Sometimes a dected defect necessitates repair of the damaged component to facilitate its further operatio
Residual stress analysis by means of neutron diffraction at research reactors facilities and applications at the HFR
Neutron diffraction is among the scientific techniques available at nuclear research reactors. A particular application of neutron diffraction is the analysis of residual stresses in crystalline materials. The basic principles of the method are described and the unique capabilities of the method are pointed out. The High Flux Reactor HFR is one of about 20 reactor based neutron sources worldwide, where instruments for this technique are available. The HFR equipment is shown and several examples of studies performed during the last decade are given. These examples include nuclear applications, investigations in automotive components and a round robin exercise executed in the context of pre normative research on the method. The examples demonstrate that the method is also suitable for validation of computational analyses or other experimental methods. A comparison between time of flight instruments and monochromatic instruments and an outlook to future developments are given at the en
Residual stress analyses by neutron diffraction in irradiated double V butt welded steel plates
Neutron irradiation is known to have a considerable impact on the mechanical characteristics and the behaviour of materials and components. The distribution of residual stresses is one of the properties affected by irradiation. However, because of the difficulties in performing measurements in radioactive components, not many experimental data have to date been collected. At the High Flux Reactor HFR of the European Commission s Joint Research Centre JRC , a facility has been developed for residual stress measurements in steel specimens subjected to longterm irradiation. The objective of this development was to establish the neutron radiation induced changes in the residual stresses around welds in test pieces representative of the core shroud of boiling water reactors. Residual Stress measurements on such double V butt welds in stainless steel plates after irradiation exposure have been performed by neutron diffraction using this facility. The comparison with measurements in non irradiated companion specimens [1] showed that irradiation changed the distribution of residual stresses. The results suggested that the impact of irradiation varied with the distance of the test location from the specimen surface. On the basis of currently measured data we could not draw definitive conclusion about the influence of neutron dose and irradiation duration upon the stress modificatio
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