FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation

We report the successful inactivation of P. aeruginosa strain by femtosecond infrared (IR) laser radiation at the resonant wavelengths of 3.15 μm and 6.04 μm, chosen due to the presence of characteristic molecular vibrations in the main structural elements of the bacterial cells in these spectral ranges: vibrations of amide groups in proteins (1500–1700 cm−1), and C-H vibrations in membrane proteins and lipids (2800–3000 cm−1). The underlying bactericidal structural molecular changes were revealed by the stationary Fourier-transform IR spectroscopy, with the spectral peaks parameters being obtained by Lorentzian fitting with the hidden peaks revealed by the second derivative calculations, while no visible damage to the cell membranes was identified by scanning and transmission electron microscopy. © 2023 by the authors.Ministry of Education and Science of the Russian Federation, MinobrnaukaThis research was funded by the Ministry of Science and Higher Education of the Russian Federation (Ural Federal University Program of Development within the Priority-2030 Program)

ORIENTATION ANALYSIS OF A PZT THIN FILM WITH 10% La BY TRANSMISSION ELECTRON MICROSCOPY

Thin PZT films are one of the prominent materials in majority of chips and devices due to their ferroelectric properties.The main goal of this paper is analysis of orientations of the structure of crystals in a thin PZT (PbZr0.53 Ti0.47 O3) film with 10% La content. For this purpose we used the stereographical projection, extinction bend contours and transmission electron microscopy

INCORPORATION OF MAGNETIC NANOPARTICLES INTO MESOPOROUS CALCIUM CARBONATE MATRICES FOR BIOMEDICAL APPLICATIONS

In this work the formation of magnetically sensitive "core-shell" systems based on pre-synthesized vaterite microparticles and iron oxide nanoparticles was studied by two methods: physical sorption of nanoparticles from a suspension at room temperature, as well as freezing-induced loading.Работа выполнена при финансовой поддержке РНФ (проект № 21-74-10058)

Digging deeper: structural background of PEGylated fibrin gels in cell migration and lumenogenesis

Fibrin is a well-known tool in tissue engineering, but the structure of its modifications created to improve its properties remains undiscussed despite its importance, e.g. in designing biomaterials that ensure cell migration and lumenogenesis. We sought to uncover the structural aspects of PEGylated fibrin hydrogels shown to contribute to angiogenesis. The analysis of the small-angle X-ray scattering (SAXS) data and ab initio modeling revealed that the PEGylation of fibrinogen led to the formation of oligomeric species, which are larger at a higher PEG : fibrinogen molar ratio. The improvement of optical properties was provided by the decrease in aggregates' sizes and also by retaining the bound water. Compared to the native fibrin, the structure of the 5 : 1 PEGylated fibrin gel consisted of homogenously distributed flexible fibrils with a smaller space between them. Moreover, as arginylglycylaspartic acid (RGD) sites may be partly bound to PEG-NHS or masked because of the oligomerization, the number of adhesion sites may be slightly reduced that may provide the better cell migration and formation of continuous capillary-like structures

Unique Self-Phosphorylating Polybenzimidazole of the 6F Family for HT-PEM Fuel Cell Application

High-temperature polymer-electrolyte membrane fuel cells (HT-PEMFCs) are a very important type of fuel cells since they operate at 150–200 °C, making it possible to use hydrogen contaminated with CO. However, the need to improve the stability and other properties of gas-diffusion electrodes still impedes their distribution. Self-supporting anodes based on carbon nanofibers (CNF) are prepared using the electrospinning method from a polyacrylonitrile solution containing zirconium salt, followed by pyrolysis. After the deposition of Pt nanoparticles on the CNF surface, the composite anodes are obtained. A new self-phosphorylating polybenzimidazole of the 6F family is applied to the Pt/CNF surface to improve the triple-phase boundary, gas transport, and proton conductivity of the anode. This polymer coating ensures a continuous interface between the anode and proton-conducting membrane. The polymer is investigated using CO2 adsorption, TGA, DTA, FTIR, GPC, and gas permeability measurements. The anodes are studied using SEM, HAADF STEM, and CV. The operation of the membrane–electrode assembly in the H2/air HT-PEMFC shows that the application of the new PBI of the 6F family with good gas permeability as a coating for the CNF anodes results in an enhancement of HT-PEMFC performance, reaching 500 mW/cm2 at 1.3 A/cm2 (at 180 °C), compared with the previously studied PBI-O-PhT-P polymer