Microbubble-mediated delivery of human adenoviruses does not elicit innate and adaptive immunity response in an immunocompetent mouse model of prostate cancer

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Lack of standardization of clinically compliant culture protocols of mesenchymal stem cells for re-implantation in humans have hindered clinical progress in the field of tissue regeneration to repair maxillofacial and orthopedic defects. The goal of this study was to establish a clinically relevant osteogenic protocol for collection and expansion of autologous stem cells to be used at Marshall University for re-implantation and repair of maxillofacial and orthopedic conditions. Human bone marrow (hBM) samples were collected from patients undergoing intramedullary nail fixation for closed femoral fractures. hBM mesenchymal cells were expanded by growing them first in Petri dishes for two weeks, followed by a week of culture using Perfecta 3D Hanging Drop Plates®. Various scaffold materials were tested and analyzed for cellular integration, vitality, and differentiation capacity of harvested hBM-MSCs including: 60/40 blend of hydroxyapatite biomatrix; Acellular bone composite discs; Allowash®, cancellous bone cubes; PLGA (poly lactic-co-glycolic acid); and Woven chitin derived fiber. We found that the 3D spheroid culture allowed production of hBM mesenchymal cells that retained osteoblast differentiation capacity over a monolayer culture of hBM-MSCs without the need to use chemical or hormonal modulation. We also observed that hydroxyapatite and Allowash cancellous bone scaffolds allowed better cell integration and viability properties as compared to other materials tested in this study. In conclusion, the multimodal culture methodology we developed creates actively differentiating stem-cell spheroids that can then be readily utilized in clinical practices to improve the regeneration of tissues of the head and the body

Efficient Genetic Method for Establishing Drosophila Cell Lines Unlocks the Potential to Create Lines of Specific Genotypes

Analysis of cells in culture has made substantial contributions to biological research. The versatility and scale of in vitro manipulation and new applications such as high-throughput gene silencing screens ensure the continued importance of cell-culture studies. In comparison to mammalian systems, Drosophila cell culture is underdeveloped, primarily because there is no general genetic method for deriving new cell lines. Here we found expression of the conserved oncogene RasV12 (a constitutively activated form of Ras) profoundly influences the development of primary cultures derived from embryos. The cultures become confluent in about three weeks and can be passaged with great success. The lines have undergone more than 90 population doublings and therefore constitute continuous cell lines. Most lines are composed of spindle-shaped cells of mesodermal type. We tested the use of the method for deriving Drosophila cell lines of a specific genotype by establishing cultures from embryos in which the warts (wts) tumor suppressor gene was targeted. We successfully created several cell lines and found that these differ from controls because they are primarily polyploid. This phenotype likely reflects the known role for the mammalian wts counterparts in the tetraploidy checkpoint. We conclude that expression of RasV12 is a powerful genetic mechanism to promote proliferation in Drosophila primary culture cells and serves as an efficient means to generate continuous cell lines of a given genotype

Optimization of a high-voltage MOSFET in ultra-thin 14nm FDSOI technology

session 6: Low voltageInternational audienc

Promoting Health Equity and COVID-19 Vaccine Uptake Through Collaboration, Outreach, Education & Engagement

The COVID-19 Health Equity program is unique in the district as it is dedicated to achieving Health Equity in our community. The program was established to address health disparities among disproportionately affected populations. Our team developed a comprehensive plan that relied on collaboration with community partnerships. The approach of the program was to provide education, testing/vaccination, and linkage to resources. Using the GDPH vaccine dashboard, the program targeted areas of high cases rates and low vaccination rates and prioritized them for education and outreach. Community resources, partners, & stakeholders were identified in those areas to assist our efforts. The staff provided education on case status, testing, vaccines, and guidance to partners and members of the community. Understanding that vaccine hesitancy is linked to lack of education, media coverage, and distrust of the government, the program leveraged community influencers and leaders to support education and outreach efforts. Testing and vaccine clinics were planned in collaboration with shelters, county governments, churches, food pantries, schools, festivals, etc. To increase vaccine uptake, monetary incentives were offered to those receiving one of the primary series vaccines. To address the spectrum of health equity, program staff linked individuals to resources available within their community. Staff educated individuals on existing programs within Public Health. With the support of established community partners, the program planned and coordinated events that provided healthcare access, tools to achieve economic stability, resources to improve the neighborhood and environments that contribute to health disparities. From its inception in April 2022, this program has planned, coordinated, or participated in over 50 community outreach events and established over 100 community partners. We expanded over-the-counter testing availability in rural communities by distributing over 1500 test kits, increased vaccination uptake by incentivizing over 400 first or second doses and facilitated 17 connections to other Public Health resources

High Critical Current Densities in Nb3Sn Films with Engineered Microstructures--Artifical Pinning Microstructures

Films with layers of Nb, Cu, and Sn have been fabricated to simulate a Nb{sub 3}Sn bronze-type process. These Nb{sub 3}Sn films have produced critical current densities greater than 1 x 10{sup 6} A/cm{sup 2} at 4.2 K and 7.5 T. Niobium films doped with Y, Sc, Dy, Al{sub 2}O{sub 3}, and Ti have been deposited with e-beam co-evaporation onto 75 mm diameter Si wafers with a 100 nm SiO{sub 2} buffer layer. The Nb layer was followed by a layer of Cu and a layer of Sn to complete the bronze-type process. The films with the highest J{sub c} had about 8 vol. % Sc and about 18 vol. % Al{sub 2}O{sub 3}. Characterization of the microstructure by TEM shows that these high J{sub c} films contained high density of inclusions about 5 nm in size and that the grain size of the Nb{sub 3}Sn is about 20-25 nm for samples heat treated at 700 C for up to eight hours

Improved modeling of isolated EDMOS in advanced CMOS technologies.

Session A - CADInternational audienc