Recombination-Based screening for genes on chromosome 21

In previous work, a reasonably large number of genes was found to be on chromosome 21. This frequency demands the creation of new techniques to investigate the transcription of this small human autosome, and to relate these findings to the phenotype of Down syndrome. Here we describe the elaboration of new vectors and hosts, which in conjunction with flow-sorted cosmid libraries of chromosome 21 and genic (cDNA) libraries of relevant human tissues, will permit us to examine transcription of chromosome 21.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38253/1/1320370723_ftp.pd

The spatial location of laser-driven, forward-propagating waves in a National-Ignition-Facility-relevant plasma

Ion acoustic and electron plasma waves, associated with backward-propagating stimulated Brillouin scattering and stimulated Raman scattering, have been diagnosed in a long-scale-length, nearly homogenous plasma with transverse flow. Thomson scattered light from a probe beam is employed to show that these waves are well localized in space and for a time much shorter than the laser pulse duration. These plasma conditions are relevant to hohlraum design for the National Ignition Facility inertial confinement fusion laser system. [R. Sawicki et al., Fusion Technol. 34, 1097 (1998)]. © 2000 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/71338/2/PHPAEN-7-1-323-1.pd

Optically induced coherent intra-band dynamics in disordered semiconductors

On the basis of a tight-binding model for a strongly disordered semiconductor with correlated conduction- and valence band disorder a new coherent dynamical intra-band effect is analyzed. For systems that are excited by two, specially designed ultrashort light-pulse sequences delayed by tau relatively to each other echo-like phenomena are predicted to occur. In addition to the inter-band photon echo which shows up at exactly t=2*tau relative to the first pulse, the system responds with two spontaneous intra-band current pulses preceding and following the appearance of the photon echo. The temporal splitting depends on the electron-hole mass ratio. Calculating the population relaxation rate due to Coulomb scattering, it is concluded that the predicted new dynamical effect should be experimentally observable in an interacting and strongly disordered system, such as the Quantum-Coulomb-Glass.Comment: to be published in Physical Review B15 February 200

Wave Packet Echoes in the Motion of Trapped Atoms

We experimentally demonstrate and systematically study the stimulated revival (echo) of motional wave packet oscillations. For this purpose, we prepare wave packets in an optical lattice by non-adiabatically shifting the potential and stimulate their reoccurence by a second shift after a variable time delay. This technique, analogous to spin echoes, enables one even in the presence of strong dephasing to determine the coherence time of the wave packets. We find that for strongly bound atoms it is comparable to the cooling time and much longer than the inverse of the photon scattering rate

Photon echoes generated by reversing magnetic field gradients in a rubidium vapour

We propose a photon echo quantum memory scheme using detuned Raman coupling to long lived ground states. In contrast to previous 3-level schemes based on controlled reversible inhomogeneous broadening that use sequences of $\pi$-pulses, the scheme does not require accurate control of the coupling dynamics to the ground states. We present a proof of principle experimental realization of our proposal using rubidium atoms in a warm vapour cell. The Raman resonance line is broadened using a magnetic field that varies linearly along the direction of light propagation. Inverting the magnetic field gradient rephases the atomic dipoles and re-emits the light pulse in the forward direction

Plasmids for recombination-based screening

To facilitate recombination-based screening, we constructed the ColE1-based plasmid, [pi]G4, that confers chloramphenicol resistance, contains a polylinker with multiple unique restriction enzyme recognition sequences, and contains the genetic marker, supF. To facilitate recombination-based screening followed by rapid DNA sequencing, we inserted the selectable marker, supF, into each of 20 high-copy-number (hcn) pUC-derived NoC plasmids that were designed for multiplex DNA sequencing. To facilitate recombination-based screening of common cDNA libraries that often contain ColE1 sequences, we constructed a supF-carrying plasmid whose replication was driven from an R6K replicon that does not share sequence homology with ColE1. Furthermore, we incorporated a useful polylinker and increased the copy number of this plasmid to create the 4.4-kb hcn plasmid, pMAD1. Thus, these plasmids allow: (1) background-free transformation of cells by a supF plasmid carrying an antibiotic-resistance marker; (2) simultaneous performance of the recombination-based assay and DNA sequencing; and (3) screening bacteriophage cDNA libraries that contain ColE1 sequences by recombination with a supF plasmid that is not homologous to ColE1 derivatives.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29122/1/0000161.pd

Measurement and control of optical nonlinearities of importance to glass laser fusion systems

Results of a number of studies carried out at Los Alamos, both experimental and theoretical, of nonlinear optical phenomena important to the design of the National Ignition Facility are summarized. These include measurements of nonlinear index coefficients, Raman scattering in atmospheric oxygen, and theoretical studies of harmonic conversion. The measurements were made by two different techniques in order to increase confidence in the results. One method was an application of a recently-developed technique for measuring the amplitude and phase of an ultrashort pulse by Frequency-Resolved Optical Gating (FROG). The other utilized a modified version of the Z-scan technique that measures beam distortion introduced by scanning a sample through the focus of a beam. The measurements by both techniques for fused silica were consistent with the lower range of previously measured values, indicating that it should not be necessary to further expand the beam size in the NIF to stay below the self-focusing threshold

Sequence-tagged sites (STSs) for a set of mapped markers on chromosome 21

Sequence tagged sites (STSs) have been proposed as a "common language" for comparing physical and genetic maps of the human genome produced by a variety of techniques. We have produced 44 STSs from 38 mapped loci on human chromosome 21. The STSs represent most of the loci designated as genetic reference or ordered physical framework markers, along with a number of others chosen to span all regions of 21q. Of the STSs, 12 are from gene segments, including 4 from exons of the APP gene encoding the amyloid [beta] protein precursor, and 32 mark anonymous DNA loci. These STSs make each of the corresponding loci readily accessible to the research community without the need for exchange of clones. These sites also represent multiple start points for the isolation of YAC clones that should permit overlapping the entire chromosome 21 long arm as cloned DNA.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29821/1/0000167.pd

Phonon-induced dephasing of localized optical excitations

The dynamics of strongly localized optical excitations in semiconductors is studied including electron-phonon interaction. The coupled microscopic equations of motion for the interband polarization and the carrier distribution functions contain coherent and incoherent contributions. While the coherent part is solved through direct numerical integration, the incoherent one is treated by means of a generalized Monte Carlo simulation. The approach is illustrated for a simple model system. The temperature and excitation energy dependence of the optical dephasing rate is analyzed and the results are compared to those of alternative approaches