Time depended inhibition of SPMs Na+ /K+ -ATPase induced by cadmium and lead

In this work, time depended interaction of SPMs Na+ /K+ -ATPase with Cd2+ and Pb2+ by single exposure and in the mixture, was investigated. The possibility to selectively detect these ions on the basis on the rate of their interaction with the protein was studied. The decreasing protein concentration increased the sensibility of Na+ /K+ - ATPase towards both metals. The selectivity was obtained by variation of metalenzyme contact time.Physical chemistry 2004 : 7th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 21-23 September 200

Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia

The isolation and molecular characterization of bacterial strains isolated from water sources in the Vlasina Mountain in southeast Serbia, confirmed the presence of a new species Chryseobacterium vrystaatense ST1. This Gram- negative species showed an extremely low level of biochemical reactivity in biochemical tests. The gene for 16S rRNA was amplified by PCR using universal primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic features indicated that the isolate ST belonged to Chryseobacterium vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA gene with all sequences deposited in the NCBI collection showed the highest similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov., designated as strain R-23533. The very high homology of these two strains allowed classification of our strain at the species level, but some differences indicate, and indirectly confirm, that the isolate ST is an authentic representative. On the basis of these results, we could conclude that Chryseobacterium vrystaatense ST was for first time isolated in Serbia, which is particularly important when one bears in mind that there are only three sequences of this species deposited in the NCBI collection

Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia

The isolation and molecular characterization of bacterial strains isolated from water sources in the Vlasina Mountain in southeast Serbia, confirmed the presence of a new species Chryseobacterium vrystaatense ST1. This Gram- negative species showed an extremely low level of biochemical reactivity in biochemical tests. The gene for 16S rRNA was amplified by PCR using universal primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic features indicated that the isolate ST belonged to Chryseobacterium vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA gene with all sequences deposited in the NCBI collection showed the highest similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov., designated as strain R-23533. The very high homology of these two strains allowed classification of our strain at the species level, but some differences indicate, and indirectly confirm, that the isolate ST is an authentic representative. On the basis of these results, we could conclude that Chryseobacterium vrystaatense ST was for first time isolated in Serbia, which is particularly important when one bears in mind that there are only three sequences of this species deposited in the NCBI collection

Molecular and biochemical characterization of pseudomonas putida isolated from bottled uncarbonated mineral drinking water

Pseudomonas putida belongs to a group of opportunistic pathogens that can cause disease in people with weakened or damaged immune systems. Some strains have medical significance, and for most ingestion is not the primary route of infection. If water used by predisposed subjects is contaminated by P. putida, they may become ill. The aim of this work was the biochemical and molecular characterization of strain ST3 of P. putida isolated from non-carbonated bottled drinking water from Jakov Do 4 on Mt. Vlasina. Characterization of P. putida was performed to assess the risk to human health of the indigenous strains present in the water. Biochemical characterization of strains was performed using the manual identification system ID 32 GN (BioMerieux). Identification was obtained using the database identification software ATB System (Bio-Merieux). Molecular characterization was performed by PCR amplification and 16S rDNA "thermal cycling sequencing". Biochemical identification of the strain ST3 was accurate (Id = 99.8%). Comparing the sequences obtained for strain ST3 with NCBI gene bank sequences for 16S rRNA, the highest similarity of our strain (96% identity) with a strain of P. putida, designated as biotype A (gi vertical bar 18076625 vertical bar emb vertical bar AJ308311.1 vertical bar.PPU308311) isolated in New Zealand, was obtained. While comparison with the NCBI collection of all deposited sequences showed that the 16S rRNA gene sequence of strain ST3 has very high homology, it is not identical, indicating indirectly that strain ST-3 is an indigenous strain

Noncanonical DNA elements in the lamin B2 origin of DNA replication

DNA replication origins of eukaryotes lack linear replicator elements but contain short (dT)(n) (dA)(n) sequences that could build mutually equivalent unorthodox structures. Here we report that the lamin B2 origin of DNA replication adopts an alternative form characterized by unpaired regions CTTTTTTTTTTCC/GGAAAAAAAAAAG (3900-3912) and CCTTTTTTTTC/GAAAAAAAAGG (4141-4151). Both unpaired regions are resistant to DNase and except in central parts of their homopyrimidine strands are sensitive to single strand-specific chemicals. Interactions that protect central pyrimidines probably stabilize the bubble-like areas. Because DNA fragments containing either one or both bubbles migrate in TBM (89 mM Tris base, 89 mM boric acid, and 2 mM MgCl2) PAGE even faster than expected from their linear size, interacting regions are expected to belong to the same molecule. In an origin fragment containing a single bubble, free homopyrimidine strand can only interact with Hoogsteen hydrogen bonding surfaces from a complementary double stranded sequence. Indeed, this origin fragment reacts with triplex preferring antibody. In competition binding experiments control double stranded DNA or single stranded (dT) 40 do not affect origin-antibody interaction, whereas TAT and GGC triplexes exert competitive effect. Because the chosen fragment does not contain potential GGC forming sequences, these experiments confirm that the lamin B2 origin adopts a structure partly composed of intramolecular TAT triads

Characterization and antimicrobial activity of natural isolate Lactococcus lactis subsp. lactis BGSM1-19

Soj Lactococcus lactis subsp. lactis BGSM1-19, izolovan iz sira tradicionalno proizvedenog u domaćinstvu, sintetiše dva bakteriocina: bakteriocin BacSMa koji pripada grupi laktokokcina B i bakteriocin BacSMb koji pokazuje visoku homologiju sa lakticinom RM. Čišćenjem plazmida, sa relativno niskim prinosom (0,33%), dobijene su dve grupe derivata: BacSMa- BacSMas derivat i BacSMa- BacSMas, BacSMb-, BacSMbs.. Sinteza bakteriocina je ispitivana tokom logaritamske faze rasta pri čemu je utvrđen maksimum proizvodnje u kulturi staroj 8 sati gajenoj na temperaturi od 30oC, što odgovara ranoj stacionarnoj fazi rasta. Biohemijska karakterizacija je ukazala da soj BGSM1-19 zadržava antimikrobnu aktivnost u opsegu pH vrednosti od 1 do 12 kao i posle tretmana na 100 oC u trajanju od 15 minuta. Utvrđeno je da se antimikrobna aktivnost potpuno gubi nakon tretmana različitim proteolitičkim enzimima. Soj BGSM1-19 poseduje pet plazmida. U eksperimentima čišćenja od plazmida utvrđeno je da se geni za sintezu i imunost na bakteriocine nalaze na plazmidima. Pored toga, BGSM1-19 pokazuje antimikrobnu aktivnost na ispitivane patogene bakterije kao što su Salmonella paratyphi, Micrococcus flavus, Pseudomonas aeruginosa i Staphylococcus aureus.The strain Lactococcus lactis subsp. lactis BGSM1-19, isolated from traditionally homemade white cheese, produces two bacteriocins: lactococcin B-like bacteriocin named bacteriocin BacSMa and bacteriocin BacSMb which have shown similarity with lacticin RM. Plasmid curing resulted in a low yield (0.33%) of BacSMa- BacSMas and BacSMa- BacSMas, BacSMb-, BacSMbs derivatives. The bacteriocin biosynthesis was observed in the logarithmic phase of growth and the production plateau was reached after 8 h of incubation at 30oC, when the culture entered the early stationary phase. Biochemical characterization showed that strain BGSM1-19 retained antimicrobial activity within the pH range of 1 to 12 or after treatment at 100oC for 15 min. However, bacteriocin activity was completely lost after treatment with different proteolytic enzymes. The strain BGSM1-19 contains five plasmids. Plasmid curing indicated that genes coding for bacteriocins synthesis and immunity seem to be located on plasmids. BGSM1-19 exhibited antimicrobial activity against some pathogenic bacteria such as Salmonella paratyphi, Micrococcus flavus, Pseudomonas aeruginosa and Staphylococcus aureus

Supplementary data for article: Kekez, B. D.; Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Stefanovic Kojic, J. R.; Markovic, M. D.; Beskoski, V. P.; Vrvic, M. M. High Levan Production by Bacillus Licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. Applied Biochemistry and Biotechnology 2015, 175 (6), 3068–3083. https://doi.org/10.1007/s12010-015-1475-8

Supplementary material for: [https://doi.org/10.1007/s12010-015-1475-8]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1670

Optimization of Microwave-Assisted synthesis of 5-hydroxymethyl-2-furfural

Heterocyclic furan-based compounds are interesting bio-renewable materials used as intermediate for pharmaceuticals, biodegradable resins, biofuels and fine chemicals syntheses. Additionally, HMF also functions as an anti-sickling agent for intermolecular sickle hemoglobin without inhibition by plasma and tissue proteins or other undesirable sequences.Poster presented at: 7 th Conference of Young Chemists of Serbia, Belgrad