A Search For Star Formation in the Smith Cloud

Motivated by the idea that a subset of HVCs trace dark matter substructure in the Local Group, we search for signs of star formation in the Smith Cloud, a nearby ~2x10^6 Msun HVC currently falling into the Milky Way. Using GALEX NUV and WISE/2MASS NIR photometry, we apply a series of color and apparent magnitude cuts to isolate candidate O and B stars that are plausibly associated with the Smith Cloud. We find an excess of stars along the line of sight to the cloud, but not at a statistically significant level relative to a control region. The number of stars found in projection on the cloud after removing an estimate of the contamination by the Milky Way implies an average star formation rate surface density of 10^(-4.8 +/- 0.3) Msun yr^(-1) kpc^(-2), assuming the cloud has been forming stars at a constant rate since its first passage through the Milky Way ~70 Myr ago. This value is consistent with the star formation rate expected based on the average gas density of the cloud. We also discuss how the newly discovered star forming galaxy Leo P has very similar properties to the Smith Cloud, but its young stellar population would not have been detected at a statistically significant level using our method. Thus, we cannot yet rule out the idea that the Smith Cloud is really a dwarf galaxy.Comment: 9 pages, 7 figures, accepted for publication in MNRA

A Broad Search for Counterrotating Gas and Stars: Evidence for Mergers and Accretion

We measure the frequency of bulk gas-stellar counterrotation in a sample of 67 galaxies drawn from the Nearby Field Galaxy Survey, a broadly representative survey of the local galaxy population down to M_B-15. We detect 4 counterrotators among 17 E/S0's with extended gas emission (24% +8 -6). In contrast, we find no clear examples of bulk counterrotation among 38 Sa-Sbc spirals, although one Sa does show peculiar gas kinematics. This result implies that, at 95% confidence, no more than 8% of Sa-Sbc spirals are bulk counterrotators. Among types Sc and later, we identify only one possible counterrotator, a Magellanic irregular. We use these results together with the physical properties of the counterrotators to constrain possible origins for this phenomenon.Comment: 19 pages, 4 figures, AJ, accepte

SIMULTANEOUS ESTIMATION AND METHOD DEVELOPMENT FOR L-CARNITINE AND METFORMIN IN HUMAN PLASMA USING LC-MS/MS

 Objectives: The objective was to develop a robust simultaneous method for quantifying L-Carnitine and metformin using liquid chromatographymass spectrometer (LCMSMS).Methods: LCMSMS method was developed considering its selectivity, sensitivity, and accuracy. Blank correction method was followed as L-Carnitineis found endogenously. Range was selected based on the Normal value for L-Carnitine and maximum concentration for metformin.Results: Rugged simultaneous method using ultra flow LC mass spectrometer for the estimation of L-Carnitine and metformin, in human plasmawith low limit of quantification and upper limit of quantification of 2.289 μg/mL and 33.675 μg/mL for L-Carnitine, 43.483 ng/ml and 639.450 ng/mlfor metformin, respectively, was developed which can be used for therapeutic drug monitoring and as a prognostic tool for Type 2 diabetes mellitus(T2DM). Extraction was optimized using Protein precipitation (PP) in which sample clean-up was simple, and recovery was consistent. Linearity waschecked and found to be within acceptance criteria with 1/×2 as the weighing factor. As L-Carnitine is present endogenously blank correction wascarried by injecting blank six times and calculated for normalization. As the extraction is by simple PP internal standard was not used, and method ismeeting the criteria for validation using LCMSMS.Conclusion: Hence, considering L-Carnitine as the biomarker for T2DM and metformin as the drug for treatment, a simultaneous method wasdeveloped which can be validated as per regulatory requirements and can be verified for the applicability of the method as a therapeutic drugmonitoring and prognostic tool.Keywords: L-Carnitine, Metformin, Type 2 diabetes, Liquid chromatography mass spectrometer

A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel

In this short communication we report a quick, cost-free method of purification of DNA fragments from agarose gel. Unlike those procedures that involve commercial kits, this method uses glass wool or absorbent cotton to filter agarose gel during a quick spinning-down of DNA, thus significantly simplifying the routine practice of many molecular biologists and decreasing the cost

The Fueling Diagram: Linking Galaxy Molecular-to-Atomic Gas Ratios to Interactions and Accretion

To assess how external factors such as local interactions and fresh gas accretion influence the global ISM of galaxies, we analyze the relationship between recent enhancements of central star formation and total molecular-to-atomic (H2/HI) gas ratios, using a broad sample of field galaxies spanning early-to-late type morphologies, stellar masses of 10^(7.2-11.2) Msun, and diverse stages of evolution. We find that galaxies occupy several loci in a "fueling diagram" that plots H2/HI vs. mass-corrected blue-centeredness, a metric tracing the degree to which galaxies have bluer centers than the average galaxy at their stellar mass. Spiral galaxies show a positive correlation between H2/HI and mass-corrected blue-centeredness. When combined with previous results linking mass-corrected blue-centeredness to external perturbations, this correlation suggests a link between local galaxy interactions and molecular gas inflow/replenishment. Intriguingly, E/S0 galaxies show a more complex picture: some follow the same correlation, some are quenched, and a distinct population of blue-sequence E/S0 galaxies (with masses below key transitions in gas richness) defines a separate loop in the fueling diagram. This population appears to be composed of low-mass merger remnants currently in late- or post-starburst states, in which the burst first consumes the H2 while the galaxy center keeps getting bluer, then exhausts the H2, at which point the burst population reddens as it ages. Multiple lines of evidence suggest connected evolutionary sequences in the fueling diagram. In particular, tracking total gas-to-stellar mass ratios within the diagram provides evidence of fresh gas accretion onto low-mass E/S0s emerging from central starbursts. Drawing on a comprehensive literature search, we suggest that virtually all galaxies follow the same evolutionary patterns found in our broad sample.Comment: 24 pages, 11 figures (table 4 available at http://user.physics.unc.edu/~dstark/table4_csv.txt), accepted for publication in Ap

Physical Sources of Scatter in the Tully-Fisher Relation

We analyze residuals from the Tully-Fisher relation for the emission-line galaxies in the Nearby Field Galaxy Survey, a broadly representative survey designed to fairly sample the variety of galaxy morphologies and environments in the local universe. For spirals brighter than M_R^i=-18, we find strong correlations between Tully-Fisher residuals and both B-R color and EW(Halpha). The extremes of the correlations are populated by Sa galaxies, which show consistently red colors, and spirals with morphological peculiarities, which are often blue. If we apply an EW(Halpha)-dependent or B-R color-dependent correction term to the Tully-Fisher relation, the scatter in the relation no longer increases from R to B to U but instead drops to a nearly constant level close to the scatter we expect from measurement errors. We argue that these results probably reflect correlated offsets in luminosity and color as a function of star formation history. Broadening the sample in morphology and luminosity, we find that most non-spirals brighter than M_R^i=-18 follow the same correlations as do spirals, albeit with greater scatter. However, the color and EW(Halpha) correlations do not apply to galaxies fainter than M_R^i=-18 or to emission-line S0 galaxies with anomalous gas kinematics. For the dwarf galaxy population, the parameters controlling Tully-Fisher residuals are instead related to the degree of recent disturbance: overluminous dwarfs have higher rotation curve asymmetries, brighter U-band effective surface brightnesses, and shorter gas consumption timescales than their underluminous counterparts. As a result, sample selection strongly affects the measured faint-end slope of the Tully-Fisher relation. Passively evolving, rotationally supported galaxies display a break toward steeper slope at low luminosities.Comment: 58 pages including 21 figures, AJ, accepte

ECO and RESOLVE: Galaxy Disk Growth in Environmental Context

We study the relationships between galaxy environments and galaxy properties related to disk (re)growth, considering two highly complete samples that are approximately baryonic mass limited into the high-mass dwarf galaxy regime, the Environmental COntext (ECO) catalog (data release herein) and the B-semester region of the REsolved Spectroscopy Of a Local VolumE (RESOLVE) survey. We quantify galaxy environments using both group identification and smoothed galaxy density field methods. We use by-eye and quantitative morphological classifications plus atomic gas content measurements and estimates. We find that blue early-type (E/S0) galaxies, gas-dominated galaxies, and UV-bright disk host galaxies all become distinctly more common below group halo mass ~10^11.5 Msun, implying that this low group halo mass regime may be a preferred regime for significant disk growth activity. We also find that blue early-type and blue late-type galaxies inhabit environments of similar group halo mass at fixed baryonic mass, consistent with a scenario in which blue early types can regrow late-type disks. In fact, we find that the only significant difference in the typical group halo mass inhabited by different galaxy classes is for satellite galaxies with different colors, where at fixed baryonic mass red early and late types have higher typical group halo masses than blue early and late types. More generally, we argue that the traditional morphology-environment relation (i.e., that denser environments tend to have more early types) can be largely attributed to the morphology-galaxy mass relation for centrals and the color-environment relation for satellites.Comment: 26 pages and 28 figures; v2 contains minor figure and text updates to match final published version in ApJ; ECO data table release now available at http://resolve.astro.unc.edu/pages/data.ph