Application of fluorescence spectroscopy and multispectral imaging for non-invasive estimation of GFP transfection efficiency

Electroporation and ultrasound induced sonoporation has been showed to induce plasmid DNA transfection to the mice tibialis cranialis muscle. It offers new prospects for gene therapy and cancer treatment. However, numerous experimental data are still needed to deliver the plausible explanation of the mechanisms governing DNA electro- or sono-transfection, as well as to provide the updates on transfection protocols for transfection efficiency increase. In this study we aimed to apply non-invasive optical diagnostic methods for the real time evaluation of GFP transfection levels at the reduced costs for experimental apparatus and animal consumption. Our experimental set-up allowed monitoring of GFP levels in live mice tibialis cranialis muscle and provided the parameters for DNA transfection efficiency determinationBiologijos katedraLietuvos sporto universitetasVytauto Didžiojo universiteta

Assessment of efficiencies of electroporation and sonoporation methods by using fluorescence RGB imaging method

Progress in biomedical optics and imaging (Vol. 15, no. 43, ISSN 1605-7422) : international conference Biophotonics Riga, Latvia, 26-31 August 2013 / edited Janis Spigulis; Ilona KuzminaSimple RGB method for fluorescence in vivo imaging is presented to assess efficiency of electroporation and sonoporation methods by measuring distribution and accumulation of green fluorescence protein (GFP) concentration. 20 laboratory measurements were performed on mice to test the methodBiologijos katedraVytauto Didžiojo universiteta

Fluorescence spectroscopy for estimation of anticancer drug sonodestruction in vitro

Progress in biomedical optics and imaging (Vol. 15, no. 43, ISSN 1605-7422) : international conference Biophotonics Riga, Latvia, 26-31 August 2013 / edited Janis Spigulis; Ilona KuzminaThe effect of ultrasound exposure on bleomycin fluorescence and pharmacological properties is studied. Bleomycin was treated by ultrasound for 7 min. Bleomycin fluorescence was measured during ultrasound exposure by means of fiber-optic spectrometry. Cell colony test was used to evaluate blemycin cytotoxity before and after ultrasound exposureBiologijos katedraVytauto Didžiojo universiteta