Degradation of the beta-alumina electrolyte in a zebra cell

Bibliography: pages 103-106.Beta-alumina solid electrolytes studied were subjected to charge and discharge cycles in a secondary, high energy density Na/beta-alumina/NaAlCl₄/FeCl₂ cell (known as the Zebra cell) at 250⁰ C. These electrolytes were studied by means of optical microscopy, as well as SEM and EDS analyses to est ablish possible failure modes. After cycling little discolouration, or impurity pickup was found to have occurred in the electrolyte. The forms of degradation of the beta-alumina electrolyte identified appeared to be a result of inherent flaws in the beta-alumina electrolyte tube, problems due to protracted storage of the tubes, or an apparent interfacial film on the cathode/electrolyte interface. A lead wetting agent was used in the cells to enhance the wetting of the beta-alumina electrolyte by the liquid Na. A study of the coating after cycling of the cell showed that the coating was adherent irrespective of the number of cycles completed. The coating did not appear to influence cracking of the electrolyte during cycling. Cracking of the beta-alumina electrolyte was found to have initiated from the Na/electrolyte interface. No evidence of crack initiation nor internal damage was found on the cathode/electrolyte interface. The cracks through the beta-alumina electrolyte wall were found to have sealed by the formation of a plug consisting largely of Na and Cl. On the basis of EDS analyses of the fracture surface of the sealed crack possible sealing mechanisms are proposed

Management of premature rupture of the membranes after 34 weeks' gestation - early versus delayed induction of labour

Objective. To determine the optimal way to manage patients with premature rupture of the membranes after 34 weeks' gestation.Design. A prospective, randomised controlled trial comparing immediate induction and delayed induction after 24 • 48 hours.Setting. Tygerberg Hospital, Gape Town.Participants. Seventy consecutive patients with premature rupture of the membranes who presented at Tygert>erg Hospital between July and October 1991.Main outcome measures. The two groups were compared with regard to infectious morbidity and antibiotic requirements in the mothers and babies, days spent in hospital, caesarean section rates, duration of labour and analgesic requirements.Results. There was no difference between the two groups in terms of infectious morbidity in either the mothers or the babies, the duration of labour or the caesarean section rates. Nine patients (26%) in the delayed induction group required analgesic treatment during labour versus 18 patients (52%) in the group that was induced immediately (P = 0.049; odds ratio = 0.327; 95% confidence limits = 0.014 - 0.0998). In the delayed induction group, 74% of the patients went into spontaneous labour during the conservative management period. Patients in the active group (immediate induction) had a statistically significant better chance of being discharged within 48 hours of admission (P = 0.028; odds ratio = 3.34; 95% confidence limits = 1.12 -10.73).Conclusions. The management of patients with premature rupture of the membranes after 34 weeks should be decided upon according to the level of antepartum and neonatal care which is available at the particular unit. Where there is adequate neonatal support and pressure on bed occupancy, immediate induction of labour should be considered, while peripheral units should consider conservative management before referral of patients

The impact of an electricity generation tax on the South African economy

In the 2008 budget of the Minister of Finance, the South African Government proposed to impose a 2 cents/kilowatt-hour (c/kWh) tax on the sale of electricity generated from non-renewable sources; this tax is to be collected at source by the producers/generators of electricity. The intention of this measure is to serve a dual purpose of protecting the environment and helping to manage the current electricity supply shortages by reducing demand. The objective here is to evaluate the impact of such an electricity generation tax on the South African, SACU and SADC economies. The paper firstly considers the theoretical foundations of an electricity generation tax supported by international experiences in this regard. This section also contrasts the suitability of a permit with a tax system to achieve CO2 emission reduction. We subsequently apply the Global Trade Analysis Project (GTAP) model to evaluate the impact of an electricity generation tax on the South African, SACU and SADC economies. We simulate the proposed tax as a 10 percent increase in the output price of electricity. We assume a closure rule that allows unskilled labour to migrate and a limited skilled workforce. As expected, the electricity generation tax will reduce demand. Due to the decrease in domestic demand, export volume increases and import volume decreases, this is despite a weaker terms of trade. We also found that unemployment for unskilled labour increases and wages of skilled workers are expected to decrease. A unilateral electricity generation tax will benefit other SACU and SADC countries through an improvement in relative competitiveness, as shown by the improvement of the terms of trade for these regions. If, however, the benefits of pollution abatement are internalised, then electricity generation tax is expected to yield a positive effect on the South African economy.

A deep gold mine metagenome as a source of novel esterases

New sources of enzymes for biotechnological applications are continually being sought for. While diverse microbial ecosysyems have been demonstrated in the deep subsurfaces, deep mines provide easy access to these specialist communities. Therefore, the aim of this study was to assess a deep mine biofilm as a source of novel esterase enzymes. Biofilm was collected from the Beatrix Mine in South Africa, at a depth of 808 m. Assessment of the diversity revealed a group of previously uncultured bacteria and archaea. A metagenome library was screened for esterolytic activity, producing two esterolytic clones: a phospholipase patatin protein and an isochorismatase family protein. The isochorismatase family protein contained the catalytic Asp and Cys but not the Arg, which is considered as important for catalysis. The patatin showed 55% similarity to its closest relative; the patatin family protein from Plesiocystis pacifica. The expressed patatin displayed a preference for the C6 ester and was maximally active at pH 8 and 30°C. This study reported that screening of a relatively small metagenome library from the deep mine biofilm provided two esterolytic clones, which differed from their known counterparts. This indicates that the deep mine ecosystems contain an untapped resource of novel and potentially useful enzymes which may have applications in chemical syntheses.Key words: Metagenome library, functional screening, lipolytic activity, patatin, isochorismatas

Pitfalls using tributyrin agar screening to detect lipolytic activity in metagenomic studies

The metagenomics approach is an efficient method for obtaining novel biocatalysts and useful genes from uncultured microorganisms within diverse environments. In this study, we constructed a metagenomic library using a South African deep mine biofilm sample. The library was screened forlipolytic activity using LB Tributyrin (TLB). Although we were able to identify 3 diverse esterase enzymes, we found that 70% of the obtained sequence data revealed the presence of enzymes and genes completely unrelated to that of lipolytic enzymes thereby highlighting the limitation of screening with TLB

Classifying network attack scenarios using an ontology

This paper presents a methodology using network attack ontology to classify computer-based attacks. Computer network attacks differ in motivation, execution and end result. Because attacks are diverse, no standard classification exists. If an attack could be classified, it could be mitigated accordingly. A taxonomy of computer network attacks forms the basis of the ontology. Most published taxonomies present an attack from either the attacker's or defender's point of view. This taxonomy presents both views. The main taxonomy classes are: Actor, Actor Location, Aggressor, Attack Goal, Attack Mechanism, Attack Scenario, Automation Level, Effects, Motivation, Phase, Scope and Target. The "Actor" class is the entity executing the attack. The "Actor Location" class is the Actor‟s country of origin. The "Aggressor" class is the group instigating an attack. The "Attack Goal" class specifies the attacker‟s goal. The "Attack Mechanism" class defines the attack methodology. The "Automation Level" class indicates the level of human interaction. The "Effects" class describes the consequences of an attack. The "Motivation" class specifies incentives for an attack. The "Scope" class describes the size and utility of the target. The "Target" class is the physical device or entity targeted by an attack. The "Vulnerability" class describes a target vulnerability used by the attacker. The "Phase" class represents an attack model that subdivides an attack into different phases. The ontology was developed using an "Attack Scenario" class, which draws from other classes and can be used to characterize and classify computer network attacks. An "Attack Scenario" consists of phases, has a scope and is attributed to an actor and aggressor which have a goal. The "Attack Scenario" thus represents different classes of attacks. High profile computer network attacks such as Stuxnet and the Estonia attacks can now be been classified through the “Attack Scenario” class

Aircraft systems architecting: a functional-logical domain perspective

Presented is a novel framework for early systems architecture design. The framework defines data structures and algorithms that enable the systems architect to operate interactively and simultaneously in both the functional and logical domains. A prototype software tool, called AirCADia Architect, was implemented, which allowed the framework to be evaluated by practicing aircraft systems architects. The evaluation confirmed that, on the whole, the approach enables the architects to effectively express their creative ideas when synthesizing new architectures while still retaining control over the process

The Impact of Surface Coal Mining on the Pre- and Post-Mining Agricultural Potential of the Grassland Biome of Southern Africa

Surface mining of coal is widespread in the grassland areas of the Mpumalanga Province in South Africa, and is the main cause of many environmental impacts in this agriculturally important region. To ensure healthy and productive vegetation after the reclamation process, disturbed soils need to be ameliorated and re-vegetated with locally adapted grass species to protect, preserve and improve soils for future agricultural use. To date, soil compaction is regarded as one of the main factors that cause severe restrictions in the effective rooting depth of plants, and the level of compaction is strongly influenced by altered soil water contents, soil texture, soil structure and often non-existing organic matter contents. Imported organic materials and enhanced plant root development may lead to reduced effects of soil compaction and an increased amount of moisture in the soil profile, which becomes available for plant growth during the growing season. This research focussed on the various amelioration aspects to alleviate and mitigate soil compaction to promote sustainable vegetation establishment and production to reinstate the agricultural potential of the disturbed environment. With associated soil amelioration, an increase in biomass production, of selected plant species was noted to be 52% higher on soil that was amended with 40 t/ha of organic amendment, in comparison to the soil that did not receive any. The correct selection of species with a known root structure and basic management requirement has, therefore, the potential to be useful in creating an organically rich, alleviated and more sustainable soil environment to ensure improved rehabilitation results of surface coal mines. It can be concluded, that sufficient data has shown that reclaimed surface coal mine soils can serve as a valuable resource to the animal production industry as well as to local communities, if managed correctly

Bacterial diversity of biofilm samples from deep mines in South Africa

The Au, Pt and diamond mines of South Africa provide access to microorganism bearing fluids emanating from fractures at depths ranging from 0.7 to 3.2 km. Due to the unique characteristic of mine environment as demonstrated by extreme pH, pressure, temperatureand/or salinity, it is anticipated that it could hold the promise for novel gene sequences and hence gene products of industrial and pharmaceutical importance. To provide insight into themicrobial diversity of mines in South Africa, biofilm samples were collected from Goldfield and diamond mines and their bacterial diversity determined using molecular approaches. 16S rRNA genes were amplified from DNA extracted from these samples using polymerase chain reaction with universal bacterial primers 27F (5’- AGA GTT TGA TCM TGG CTC AG-3’) and 1492R (5’- GGT TAC CTT GTT ACG ACT T-3’). Metagenomic clone libraries were constructed and restriction fragment length polymorphism (RFLP) analysis of >100 derivedclones resulted in four major restriction patterns from which 40 clones were chosen for sequencing. More than half (53%) of the sequences were affiliated with the bacterial phylum Proteobacteria, forty-one percent (41%) of the sequences with yet uncultured bacteria andthe phyla Firmicutes and Planctomycetes were accounted for by 4% and 2% of the sequences respectively. DGGE analysis of PCR-amplified 16S rRNA genes showed characteristic fingerprints for each sample. The differences in community structure observed account for the uniqueness of each of the mines with respect to its microbial diversity