Some properties of testicular hyaluronidases

The properties (pHopt, Topt, Km, temperature, acid and base stability of the enzyme activity) of hyaluronidase prepared from testes of bovine, horse, pig and antelope were determined.DOI: http://dx.doi.org/10.5564/mjc.v12i0.169 Mongolian Journal of Chemistry Vol.12 2011: 38-4

Some properties of testicular hyaluronidases

The properties (pHopt, Topt, Km, temperature, acid and base stability of the enzyme activity) of hyaluronidase prepared from testes of bovine, horse, pig and antelope were determined.DOI: http://dx.doi.org/10.5564/mjc.v12i0.169 Mongolian Journal of Chemistry Vol.12 2011: 38-4

Phylogenetic analysis of mitochondrial 12S ribosomal RNA gene sequence of Mongolian wild boars

Specimens of Mongolian wild boar (Sus scrofa) from Arkhangai, Dornod, Zavkhan, Orkhon, Ovorkhangai, Selenge, Tuv, Khovd, Khuvsgul and Uvs aimags (provinces) were subjected to DNA sequencing. Determined sequences from 18 specimens were registered into the GenBank and accession numbers were obtained. In this study mitochondrial 12S rRNA gene sequences of Mongolian wild boars were analyzed with 36 complete sequences of 12S rRNA gene of wild boar (Sus scrofa) available at NCBI GenBank. Sequence alignment, detection of parsimonious informative sites, model selection, calculation of nucleotide distances and tree construction with 1000 bootstrapped replications were conducted using MEGA 6. Maximum likelihood trees were constructed by the HKY model. A maximum likelihood tree with 53 complete sequences of mitochondrial 12S rRNA gene of Sus scrofa was constructed. Mongolian sequences from the same and adjacent locations were clustered together. European sequences were clustered together, additionally two sequences from south western China and two sequences from south eastern China were also clustered. Additionally, 12S rRNA gene sequences of Mongolian Sus scrofa, located between Asian and European sequences suggesting geographical location of Mongolia, played an important role in the gene flow between Asian and European wild boar population

Phylogenetic analysis of mitochondrial 12S ribosomal RNA gene sequence of Mongolian wild boars

Specimens of Mongolian wild boar (Sus scrofa) from Arkhangai, Dornod, Zavkhan, Orkhon, Ovorkhangai, Selenge, Tuv, Khovd, Khuvsgul and Uvs aimags (provinces) were subjected to DNA sequencing. Determined sequences from 18 specimens were registered into the GenBank and accession numbers were obtained. In this study mitochondrial 12S rRNA gene sequences of Mongolian wild boars were analyzed with 36 complete sequences of 12S rRNA gene of wild boar (Sus scrofa) available at NCBI GenBank. Sequence alignment, detection of parsimonious informative sites, model selection, calculation of nucleotide distances and tree construction with 1000 bootstrapped replications were conducted using MEGA 6. Maximum likelihood trees were constructed by the HKY model. A maximum likelihood tree with 53 complete sequences of mitochondrial 12S rRNA gene of Sus scrofa was constructed. Mongolian sequences from the same and adjacent locations were clustered together. European sequences were clustered together, additionally two sequences from south western China and two sequences from south eastern China were also clustered. Additionally, 12S rRNA gene sequences of Mongolian Sus scrofa, located between Asian and European sequences suggesting geographical location of Mongolia, played an important role in the gene flow between Asian and European wild boar population

Study on synthesizing Mg/Al layered double hydroxides at different pHs

Mg/Al layered double hydroxide (LDH) was successfully synthesized at different pHs values. The Mg/AL LDH was well characterized by X-Ray diffraction and Fourier transform infrared analysis. The morphology of the LDH was observed using Scanning electron microscopy with energy dispersive X-ray spectroscopy. The influence of pH values on the morphology of the Mg/Al LDHs were studied. The result showed that the well-synthesized Mg/Al LDHs could be obtained when the pH value was about 10.0 at room temperature.DOI: http://doi.dx.org/10.5564/mjc.v15i0.319 Mongolian Journal of Chemistry 15 (41), 2014, p36-3

Study on synthesizing Mg/Al layered double hydroxides at different pHs

Mg/Al layered double hydroxide (LDH) was successfully synthesized at different pHs values. The Mg/AL LDH was well characterized by X-Ray diffraction and Fourier transform infrared analysis. The morphology of the LDH was observed using Scanning electron microscopy with energy dispersive X-ray spectroscopy. The influence of pH values on the morphology of the Mg/Al LDHs were studied. The result showed that the well-synthesized Mg/Al LDHs could be obtained when the pH value was about 10.0 at room temperature.DOI: http://doi.dx.org/10.5564/mjc.v15i0.319 Mongolian Journal of Chemistry 15 (41), 2014, p36-3

Lipoprotein(a) and HIV: Allele-specific apolipoprotein(a) levels predict carotid intima-media thickness in hiv-infected young women in the women's interagency HIV study

Objective - In the general population, lipoprotein(a) [Lp(a)] has been established as an independent causal risk factor for cardiovascular disease. Lp(a) levels are to a major extent regulated by a size polymorphism in the apolipoprotein(a) [apo(a)] gene. The roles of Lp(a)/apo(a) in human immunodeficiency virus (HIV)-related elevated cardiovascular disease risk remain unclear. Approach and Results - The associations between total plasma Lp(a) level, allele-specific apo(a) level, an Lp(a) level carried by individual apo(a) alleles, and common carotid artery intima-media thickness were assessed in 150 HIV-infected and 100 HIV-uninfected women in the WIHS (Women's Interagency HIV Study). Linear regression analyses with and without adjustments were used. The cohort was young (mean age, ≈31 years), with the majority being Blacks (≈70%). The prevalence of a small size apo(a) (≤22 Kringle repeats) or a high Lp(a) level (≥30 mg/dL) was similar by HIV status. Total plasma Lp(a) level (P=0.029) and allele-specific apo(a) level carried by the smaller apo(a) sizes (P=0.022) were significantly associated with carotid artery intima-media thickness in the HIV-infected women only. After accounting for confounders (age, race, smoking, body mass index, blood pressure, hepatitis C virus coinfection, menopause, plasma lipids, treatment status, CD4+ T cell count, and HIV/RNA viral load), the association remained significant for both Lp(a) (P=0.035) and allele-specific apo(a) level carried by the smaller apo(a) sizes (P=0.010) in the HIV-infected women. Notably, none of the other lipids/lipoproteins was associated with carotid artery intima-media thickness. Conclusions - Lp(a) and allele-specific apo(a) levels predict carotid artery intima-media thickness in HIV-infected young women. Further research is needed to identify underlying mechanisms of an increased Lp(a) atherogenicity in HIV infection