874 research outputs found
Anti-dumping duties and the Byrd amendment.
The Byrd amendment to US anti-dumping law distributes the revenue from anti-dumping duties imposed on foreign firms to the domestic firms that lodged the complaint of dumping. When the government sets its anti-dumping duty to maximise a welfare function that attaches greater weight to the profits of the domestic industry than to consumer surplus or tax revenue, it is shown that the Byrd amendment will lead to lower duties and higher welfare if the weight on the profits of the domestic industry is sufficiently large. Also, the Byrd amendment makes it less likely that the anti-dumping duty will be prohibitive.Anti dumping; Research; Law; Firms; Domestic; Dumping; Welfare; Industry;
Evaluation of association of blood and bronchoalveolar eosinophil numbers and serum total immunoglobulin E concentration with the expression of nonspecific airway reactivity in dogs
Optimal Conditions for Kinetic Study of Succinate Dehydrogenase in Rat Liver
Succinate dehydrogenase (SDH) commonly is assayed as a marker enzyme for mitochondrial activity. The literature presents numerous conditions for conducting this assay due to the fact that, it has been difficult to get sufficient reduction of the acceptor dye, 2,3,5-triphenyl-2H-tetrazolium chloride (TTC). This study was undertaken to optimize the SDH-catalyzed reduction of TTC dye by evaluation of a greater range of molor ratios of TTC to succinate and by further evaluation of additives reported as beneficial. Improvement in enzyme specific activity was achieved by liver perfusion via the left cardiac ventricle with homogenizing solution. Increase in TTC from 1 to 10 mM and further increase to 20 mM resulted in major improvement in color production. The greatest improvement in apparent activity was achieved by addition of 1 mM phenozine methosulfate, a hydrogen transfer mediator. Use of CaCI₂. EDTA, Triton X-100, NaN₃ and KCN was not beneficial. The above modifications of the SDH assay resulted in greater sensitivity, the conduct of a greater number of assays with less tissue and the sacrifice of fewer animals
Pathophysiological correlations in maedi: a chronic lymphoid interstitial pneumonia of sheep
Maedi is a chronic lymphoid interstitial pneumonia (LIP) of sheep caused by the lentivirus,
maedi-visna virus (MW). Following a long prepatent phase maedi progresses to clinical disease
characterised by exercise intolerance and dyspnoea. Although maedi is well characterised at a
pathological level, physiological studies of the disease have not been undertaken. As a consequence,
the nature of the functional abnormality that underlies the clinical disease is unknown, whether there
exists measurable functional deficit in the preclinical phase of the disease is unknown and the
relationship between lung pathology and functional deficit is unknown. These questions are
fundamental to understanding the way in which pathological events ultimately conspire to bring
about organ dysfunction and clinical disease. Further, knowledge of the way in which pathology
relates to measurable lung dysfunction offers a potential means of assessing the progress and
prognosis of this disease. This thesis describes an investigation into the pathophysiological
mechanisms responsible for inducing lung functional deficits in maedi.As a prelude to establishing the nature of the functional deficit in maedi, repeated
measurements of static lung compliance (Cst), lung distensibility (K), effective alveolar volume
(VA,eff) and transfer factor for carbon monoxide (TL,CO,'sb') were made in anaesthetized control
sheep, seronegative for MW, over a period of 5 months. This study furnished regression equations
and prediction intervals for lung function indices in normal sheep using bodyweight as the
independent variable. By comparison with predicted normal values sheep naturally infected with
MW had reduced lung volumes and gas diffusing capabilities and increased lung elastic recoil.A pathophysiological study was instigated to identify structural correlates of lung
functional deficits. Preliminary investigation involved the quantitative morphometric
characterisation of the normal sheep lung. Data from this study indicated that the ratio of fixed to
physiological lung volume ranged from 0.49 to 0.59 and that this ratio was positively correlated
with the time between euthanasia and inflation fixation of the lungs. Values for tissue volume
fraction within the lung parenchyma (Fvt) ranged from 0.18 to 0.25 and values for alveolar surface
density (Svt) ranged from 592 to 716 cm2/cm3. Pathophysiological correlations in MW-infected
sheep indicated that lung volume and transfer factor measurements were more sensitive indices of
pathology than measurements of Cst or K. Transfer factor was reduced even in sheep with minimal
histopathology suggesting this index as a sensitive means of assessing this condition. The density of
surface forces could not account for variation in K seen in vivo, however tissue factors such as the
quantity and functional tone of contractile tissue in the parenchyma, airways or blood vessels may
contribute. Given that parenchymal smooth muscle hyperplasia is a pathological feature of maedi, it
was hypothesized that this tissue element is responsible for the observed reduction in K.In order to further investigate this relationship, the distribution and morphometric
quantitation of a-smooth muscle actin (ASMA) in lung parenchyma from normal and MW-infected
4
sheep was determined and related to in vivo functional measurements. The volume density of
ASMA (IVASMA1) was negatively correlated with K and Cst, however partial correlation
coefficients indicated that IVASMA' and Fvt were strongly interdependent thus complicating
interpretation of the link between Fv'ASMA' and K. In order to separate the influence of dynamic
and passive tissue elements, histamine and clenbuterol were administered to normal and MWinfected
sheep in an attempt to cause relaxation and contraction of parenchymal contractile tissue.
The functional response of the cardiopulmonary system to intravenous infusion of these agents was
measured and correlated with Fv'ASMA'. K and Cst were significantly increased following
clenbuterol injection, however only the increase in K was correlated with the quantity of Fv'ASMA',
and this correlation was negative. These results could be explained if the site of action of
clenbuterol was not the contractile tissue at the level of the alveolar ducts, but rather that which
surrounds conducting airways.The dose of histamine required to lower dynamic compliance to 65%
of baseline values was negatively correlated with Fv'ASMA' and the attendant percentage change in
K was positively correlated with Fv'ASMA'. These findings support the contention that parenchymal
contractile tissue is of functional relevance and capable of regulating overall lung elastic properties.Maedi is a naturally occurring disease in sheep in which the aetiologic agent and target cell
is known and in which the pathology is well characterised. As such it has potential as a model for
LIP associated with human immunodeficiency virus infection in children and adults. The present
study has both served to establish the functional characteristics of this disease and indicate structural
correlates of observed functional deficits. Moreover, evidence is presented to suggest that the
observed reduction in lung distensibility in maedi is a consequence of increased tissue forces
associated with the parenchymal smooth muscle hyperplasia that is a feature of this disease
Trappin ovine molecule (TOM), the ovine ortholog of elafin, is an acute phase reactant in the lung
BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep
The response of S-phase cells labelled with bromodeoxyuridine (BrdU) in sheep airways undergoing repair in response to endobronchial brush biopsy was investigated in this study. Separate sites within the airway tree of anaesthetised sheep were biopsied at intervals prior to pulse labelling with BrdU, which was administered one hour prior to euthanasia. Both brushed and spatially disparate unbrushed (control) sites were carefully mapped, dissected, and processed to facilitate histological analysis of BrdU labelling. Our study indicated that the number and location of BrdU-labelled cells varied according to the age of the repairing injury. There was little evidence of cell proliferation in either control airway tissues or airway tissues examined six hours after injury. However, by days 1 and 3, BrdU-labelled cells were increased in number in the airway wall, both at the damaged site and in the regions flanking either side of the injury. Thereafter, cell proliferative activity largely declined by day 7 after injury, when consistent evidence of remodelling in the airway wall could be appreciated. This study successfully demonstrated the effectiveness of in vivo pulse labelling in tracking cell proliferation during repair which has a potential value in exploring the therapeutic utility of stem cell approaches in relevant lung disease models
Cholecystobronchocolic Fistula: A Late Complication of Biliary Sepsis
A case of a 48 year old woman presenting with bilioptysis due to a cholecystobronchocolic fistula is
reported. Bilioptysis is a rare complication of biliary fistulae, with a high mortality due to chemical
pneumonitis. Bronchospasm and rapid respiratory failure may ensue if aggressive management is not
adopted. The site of fistulation is established by cholangiography, preferably by the percutaneous
transhepatic route. Continued biliary drainage can lead to closure of these fistulae, or allow sufficient
improvement in clinical condition to allow definitive surgery to be performed electively
Local lung responses following endobronchial elastase and lipopolysaccharide instillation in sheep
Chronic lipopolysaccharide (LPS) exposure may contribute to the pathogenesis of a number of lung diseases including COPD and emphysema. We sought to develop a large- animal model of emphysema using repeated LPS administration into sheep lung segments. An experimental protocol was designed to facilitate comparisons with elastase-treated and control segments within the same lung of individual sheep. Histopathologic evaluation of segments treated with LPS demonstrated low-grade inflammation characterized by an increase in the number of intra-alveolar macrophages and lymphocytes. Treated segments demonstrated a significant reduction in airspace surface area (ASA), an increase in percent disrupted alveolar attachments and the distance between normal alveolar attachments, and a reduction in the number of normal alveolar attachments surrounding nonrespiratory bronchioles. Coefficient of variation of individual ASA measurements in elastase-treated segments was indicative of a heterogeneous parenchymal response, in contrast to that associated with chronic LPS treatment. Our results demonstrate that chronic LPS treatment of individual lung segments in sheep induces microscopic emphysema qualitatively and quantitatively consistent with both accepted pathologic definitions of this condition and with that produced by airway instillation of elastolytic enzymes. Development of this phenotype is associated with evidence of downregulated activation of transforming growth factor beta
- …