Iowa's Turkey Industry: An Economic Review

The Iowa turkey industry is an important agricultural value-added activity providing income and employment opportunities for rural areas in Iowa. The 8.54 million turkeys produced and more than 14 million birds processed in Iowa each year require nearly 2000 full-time equivalent employees. Annual wages and salaries total an estimated $55.6 million of wages and salaries for processing and production. When all direct and secondary effects are considered, the total impacts include$810.7 million of sales, $158.7 million of personal income,$253.3 million of contribution to the gross state product, and about 4,200 jobs.

Project for the analysis of technology transfer Annual report, 1969

Technology utilization of NASA programs and other research and development programs in Federal Government - project analysis results of technology transfe

Exact results for the 1D interacting mixed Bose-Fermi gas

The exact solution of the 1D interacting mixed Bose-Fermi gas is used to calculate ground-state properties both for finite systems and in the thermodynamic limit. The quasimomentum distribution, ground-state energy and generalized velocities are obtained as functions of the interaction strength both for polarized and non-polarized fermions. We do not observe any demixing instability of the system for repulsive interactions.Comment: 12 pages, 4 figures, better comparison with hydrodynamic approach, typos corrected, references added, improved figure

Chromatin Immunoprecipitation (ChIP): Revisiting the Efficacy of Sample Preparation, Sonication, Quantification of Sheared DNA, and Analysis via PCR

The “quantitative” ChIP, a tool commonly used to study protein-DNA interactions in cells and tissue, is a difficult assay often plagued with technical error. We present, herein, the process required to merge multiple protocols into a quick, reliable and easy method and an approach to accurately quantify ChIP DNA prior to performing PCR. We demonstrate that high intensity sonication for at least 30 min is required for full cellular disruption and maximum DNA recovery because ChIP lysis buffers fail to lyse formaldehyde-fixed cells. In addition, extracting ChIP DNA with chelex-100 yields samples that are too dilute for evaluation of shearing efficiency or quantification via nanospectrophotometry. However, DNA extracted from the Mock-ChIP supernatant via the phenol-chloroform-isoamyl alcohol (PCIA) method can be used to evaluate DNA shearing efficiency and used as the standard in a fluorescence-based microplate assay. This enabled accurate quantification of DNA in chelex-extracted ChIP samples and normalization to total DNA concentration prior to performing real-time PCR (rtPCR). Thus, a quick ChIP assay that can be completed in nine bench hours over two days has been validated along with a rapid, accurate and repeatable way to quantify ChIP DNA. The resulting rtPCR data more accurately depicts treatment effects on protein-DNA interactions of interest