Heterogeneity of helper/inducer T lymphocytes. II. Effects of interleukin 4- and interleukin 2-producing T cell clones on resting B lymphocytes.

To compare the helper function of murine T cell clones that secrete IL-2 and IFN-gamma (Th1 cells) or IL-4 and IL-5 (Th2), purified resting B cells were stimulated with F(ab')2 rabbit anti-mouse Ig (RAMG) and rabbit Ig-specific, class II MHC-restricted cloned T cells belonging to the two subsets. Both Th2 clones examined induced strong proliferative responses of B cells in the presence of RAMG, as well as the secretion of IgM and IgG1 antibodies. In contrast, the Th1 clones tested failed to stimulate B cell growth or antibody secretion. Th2-mediated B cell activation was dependent on IL-4 and IL-5, and was also inhibited by IFN-gamma or IFN-gamma produced by Th1 cells present in the same cultures. However, the failure of Th1 cells to help resting B cells could not be reversed with neutralizing anti-IFN-gamma antibody. In addition to this inhibitory effect, IFN-gamma was required for the secretion of IgG2a antibody, particularly when B cells were stimulated with polyclonal activators such as LPS. Finally, both sets of T cell clones secreted lymphokines when stimulated with purified B cells and RAMG. These experiments demonstrate that T cells that differ in lymphokine production also differ in their ability to help B cells as a result of cognate interactions at low concentrations of antigens. Moreover, IL-4, IL-5, and IFN-gamma serve different roles in the T cell-dependent proliferative and differentiative responses of resting B lymphocytes

Shooting our wildlife: an analysis of the law and policy governing the killing of kangaroos

Although we kill around 3 million kangaroos annually, the current widespread commercial and non-commercial killing of kangaroos has yet to be proven necessary according to this report. Over the last decade in Australia, the Federal and State governments have approved an annual commercial kill of some four to six million kangaroos and wallabies each year. On average three million kangaroos are actually ‘harvested’/killed.2 Around three hundred thousand young at foot and 800,000 pouch young are either killed or left to die each year as collateral of the commercial industry. In addition, up to 200,000 kangaroos and wallabies are killed for non-commercial reasons each year. A further unknown number are killed without government authorisation. This is the largest land-based slaughter of wildlife in the world. This report provides an analysis of the law and policy governing the killing of kangaroos. It provides an historical outline of kangaroo killing in Australia and examines the reasons for and against the kill. The report describes and analyses the legislative and regulatory framework governing the killing of kangaroos, in order to assist policymakers understand an area which is complex and often misunderstood. The conclusion of this report is that the current widespread commercial and non-commercial killing of kangaroos has yet to be proven necessary. Governments do not provide any clear justification for the killing such as through a proper consideration of the reasons for and against control. State governments once treated kangaroos as agricultural pests yet today they are treated as a resource. These practices result in poor welfare outcomes for many kangaroos and joeys and may pose a risk to Australia’s sustainability

Radiogenomic Analysis to Predict Response to Immunotherapy in Patients with NSCLC

https://openworks.mdanderson.org/sumexp21/1243/thumbnail.jp

Effect of V and N on the microstructure evolution during continuous casting of steel

Low Carbon (LC) steel is not expected to be sensitive to hot tearing and/or cracking while microalloyed steels are known for their high cracking sensitivity during continuous casting. Experience of the Direct Sheet Plant caster at Tata Steel in Ijmuiden (the Netherlands), seems to contradict this statement. It is observed that a LC steel grade has a high risk of cracking alias hot tearing, while a High Strength Low Alloyed (HSLA) steel has a very low cracking occurrence. Another HSLA steel grade, with a similar composition but less N and V is however very sensitive to hot tearing. An extreme crack results in a breakout. A previous statistical analysis of the breakout occurrence reveals a one and a half times higher possibility of a breakout for the HSLA grade compared to the LC grade. HSLA with extra N, V shows a four times smaller possibility of breakout than LC. This study assigns the unexpected effect of the chemical composition on the hot tearing sensitivity to the role of some alloying elements such as V and N as structure refiners.This research was carried out under project number M41.5.08320 within the framework of the Research Program of the Materials innovation institute M2i (www.m2i.nl)

The role of inspections in the commercial kangaroo industry

This article provides an assessment of the enforcement of the law governing commercial kangaroo killing, focusing particularly upon inspectorial practices. Australias kangaroo industry is the largest commercial kill of land-based wildlife in the world. Professional shooters hunt kangaroos in rural and remote locations at night. Due to the remote and decentralised nature of the killing, the industry presents unique challenges to law enforcement agencies that are responsible for the enforcement of animal welfare standards. This article focuses upon the role that inspections have in detecting offences within the commercial kangaroo industry. It provides a comparative analysis across the states, highlighting key differences in terms of inspectorial practices and the resulting outcomes. A common theme across all of the jurisdictions is that none of the agencies responsible for enforcement regularly conduct inspections of shooters, making it impossible to ensure that these parties are complying with the National Code of Practice for the Humane Shooting of Kangaroos and Wallabies. Recommendations for reform are offered, including stronger compliance policy, higher rates of inspection, increased resourcing and the introduction of alternative methods of inspection

'Pest' and resource: A legal history of Australia's kangaroos

This paper presents an investigation into the legal history of Australias kangaroos. It aims to provide a detailed analysis of how the law and policy governing the killing of kangaroos has evolved over time in response to changing public perceptions. This history begins with the pre-European period and traces the impact of European colonisation, early growth of the commercial kangaroo industry, and the increased role of science and regulation upon kangaroos. The paper critiques the historical designation of kangaroos as `pests that need to be `managed and argues that such an approach is inconsistent with current scientific understanding. As this `pest status has fallen in importance there has been a shift in regulatory goals from damage mitigation to resource utilisation, although government planning and policy continue to cite damage mitigation alongside objectives to maintain viable populations and a sustainable and commercially viable industry. While the kangaroo industrys current focus is upon the `sustainable use of wildlife, the history of attitudes towards kangaroos as `pests is so deeply and widely entrenched that it is impossible for the industry to meet welfare standards. The article concludes that the commercial kangaroo industry does not have any clearly defined policy benefit and should be reassessed to take greater account of the impact it has on ecosystems and kangaroo welfar