TDZ-induced plant regeneration in Astragalus cicer L.

We developed a regeneration protocol using thidiazuron (TDZ) with a high frequency in vitro root induction in Astragalus cicer. High in vitro germination ratio (75%) for hard-seeds of A. cicer was also achieved. For this, hypocotyl and cotyledon explants were cultured on Murashige and Skoog medium supplemented with different concentrations of TDZ. The highest frequency of shoot regeneration (53.3%) was achieved from hypocotyl segments through an initial callus growth stage on MS mediumcontaining 0.25 mg/l TDZ. The shoots were cultured on the different strength (1/1, 3/4, 1/2 and 1/4) of basal Murashige and Skoog medium containing different concentrations of NAA. High rooting (100%)and survival (100%) were achieved using half strength MS medium supplemented with 0.25 and 0.50 mg/l NAA

TDZ x IBA induced shoot regeneration from cotyledonary leaves and in vitro multiplication in safflower (Carthamus tinctorius L.)

A high frequency adventitious shoot regeneration protocol for safflower (Carthamus tinctorius L.) cv. Dinçer with high yield (Turkish cultivar) using cotyledonary leaves of 10 day-old seedlings were optimized by studying the influence of different combinations of thidiazuron (TDZ) and indole-3-butyric acid (IBA). Cotyledonary leaves were cultured on Murashige and Skoog medium (MS) supplemented with different concentrations and combinations of TDZ and IBA. The highest percentage of regenerated shoots (33.33%) and the highest number of shoots per explant (6.5) occurred on a MS medium containing 0.5 mg/l TDZ and 0.25 mg/l IBA. Furthermore, cotyledonary nodes and meristem tips of 14 day-old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of N6-benzylamino-purine (BAP) alone or combination of -naphthalene acetic acid (NAA). Direct multiple shoots from cotyledonary nodes and meristem tips developed within 18 - 21 days in most media tested. 100% shoot multiplication was achieved from cotyledonary node and meristem tip on a range of MS media supplemented with different concentrations of BAP and NAA