Reflection of intense laser light from microstructured targets as a potential diagnostic of laser focus and plasma temperature

The spatial-intensity profile of light reflected during the interaction of an intense laser pulse with a microstructured target is investigated experimentally and the potential to apply this as a diagnostic of the interaction physics is explored numerically. Diffraction and speckle patterns are measured in the specularly reflected light in the cases of targets with regular groove and needle-like structures, respectively, highlighting the potential to use this as a diagnostic of the evolving plasma surface. It is shown, via ray-tracing and numerical modelling, that for a laser focal spot diameter smaller than the periodicity of the target structure, the reflected light patterns can potentially be used to diagnose the degree of plasma expansion, and by extension the local plasma temperature, at the focus of the intense laser light. The reflected patterns could also be used to diagnose the size of the laser focal spot during a high-intensity interaction when using a regular structure with known spacing

Dual action antifungal small molecule modulates multidrug efflux and TOR signaling.

There is an urgent need for new strategies to treat invasive fungal infections, which are a leading cause of human mortality. Here, we establish two activities of the natural product beauvericin, which potentiates the activity of the most widely deployed class of antifungal against the leading human fungal pathogens, blocks the emergence of drug resistance, and renders antifungal-resistant pathogens responsive to treatment in mammalian infection models. Harnessing genome sequencing of beauvericin-resistant mutants, affinity purification of a biotinylated beauvericin analog, and biochemical and genetic assays reveals that beauvericin blocks multidrug efflux and inhibits the global regulator TORC1 kinase, thereby activating the protein kinase CK2 and inhibiting the molecular chaperone Hsp90. Substitutions in the multidrug transporter Pdr5 that enable beauvericin efflux impair antifungal efflux, thereby impeding resistance to the drug combination. Thus, dual targeting of multidrug efflux and TOR signaling provides a powerful, broadly effective therapeutic strategy for treating fungal infectious disease that evades resistance

Optimization of a laser plasma-based x-ray source according to WDM absorption spectroscopy requirements

X-ray absorption spectroscopy is a well-accepted diagnostic for experimental studies of warm dense matter. It requires a short-lived X-ray source of sufficiently high emissivity and without characteristic lines in the spectral range of interest. In the present work, we discuss how to choose an optimum material and thickness to get a bright source in the wavelength range 2 Å-6 Å (∼2 keV to 6 keV) by considering relatively low-Z elements. We demonstrate that the highest emissivity of solid aluminum and silicon foil targets irradiated with a 1-ps high-contrast sub-kJ laser pulse is achieved when the target thickness is close to 10 μm. An outer plastic layer can increase the emissivity even further

Absolute keV X-ray yield and conversion efficiency in over dense Si sub-petawatt laser plasma

Laser-produced plasmas are bright, short sources of X-rays commonly used for time-resolved imaging and spectroscopy. Their usage implies accurate knowledge of laser-to-X-ray conversion efficiency, spectrum, photon yield and angular distribution. Here we report on soft X-ray emission in the direction close to the target normal from a thin Si foil irradiated by a sub-PW picosecond laser pulse. These absolute measurements cover a continuous and broad spectral range that extends from 4.75 to 7.5 Å(1.7–2.6 keV). The X-ray spectrum consists of spectral line transitions from highly charged ions and broadband emission with contributions from recombination and free-free processes that occur when electrons decelerate in plasma electromagnetic fields. Angular distribution of the emission was investigated via PIC simulations, which allowed to estimate the yield into the full solid angle. We found that experiment and simulation estimations of laser to free-free emission conversion efficiency are in a good agreement