1 research outputs found
Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentations
Background: Enzymatic activity and laccase isoenzymes number of
Pleurotus ostreatus grown in different pH values of the growing
medium in submerged fermentation and incubated in buffer solutions of
different initial pH values were determined. The expression profiles of
five laccase genes (Lacc1, Lacc4, Lacc6, Lacc9 and Lacc10) in these
cultures were also studied. Results: The highest laccases activity was
obtained in cultures grown at initial pH of 4.5 and the lowest in
cultures grown at initial pH of 8.5. Isoenzyme profiles were different
in all the cases. Lacc1, Lacc4, Lacc6 and Lacc10 were expressed in all
the cultures. Conclusions: The initial pH of the growing medium is an
important factor for regulating the expression of laccase genes, having
an effect on the activity and on the laccase isoenzymes number produced
by P. ostreatus in SmF. This is the first report on the influence of
different initial pH values of the growing medium on the laccases
activity, laccase isoenzymes number and laccases expression profiles of
P. ostreatus grown in submerged fermentation