Pre-main sequence stars with disks in the Eagle Nebula observed in scattered light

NGC6611 and its parental cloud, the Eagle Nebula (M16), are well-studied star-forming regions, thanks to their large content of both OB stars and stars with disks and the observed ongoing star formation. We identified 834 disk-bearing stars associated with the cloud, after detecting their excesses in NIR bands from J band to 8.0 micron. In this paper, we study in detail the nature of a subsample of disk-bearing stars that show peculiar characteristics. They appear older than the other members in the V vs. V-I diagram, and/or they have one or more IRAC colors at pure photospheric values, despite showing NIR excesses, when optical and infrared colors are compared. We confirm the membership of these stars to M16 by a spectroscopic analysis. The physical properties of these stars with disks are studied by comparing their spectral energy distributions (SEDs) with the SEDs predicted by models of T-Tauri stars with disks and envelopes. We show that the age of these stars estimated from the V vs. V-I diagram is unreliable since their V-I colors are altered by the light scattered by the disk into the line of sight. Only in a few cases their SEDs are compatible with models with excesses in V band caused by optical veiling. Candidate members with disks and photospheric IRAC colors are selected by the used NIR disk diagnostic, which is sensitive to moderate excesses, such as those produced by disks with low masses. In 1/3 of these cases, scattering of stellar flux by the disks can also be invoked. The photospheric light scattered by the disk grains into the line of sight can affect the derivation of physical parameters of ClassII stars from photometric optical and NIR data. Besides, the disks diagnostic we defined are useful for selecting stars with disks, even those with moderate excesses or whose optical colors are altered by veiling or photospheric scattered light.Comment: Accepted for publication in A&

Mineral Processing by Short Circuits in Protoplanetary Disks

Meteoritic chondrules were formed in the early solar system by brief heating of silicate dust to melting temperatures. Some highly refractory grains (Type B calcium-aluminum-rich inclusions, CAIs) also show signs of transient heating. A similar process may occur in other protoplanetary disks, as evidenced by observations of spectra characteristic of crystalline silicates. One possible environment for this process is the turbulent magnetohydrodynamic flow thought to drive accretion in these disks. Such flows generally form thin current sheets, which are sites of magnetic reconnection, and dissipate the magnetic fields amplified by a disk dynamo. We suggest that it is possible to heat precursor grains for chondrules and other high-temperature minerals in current sheets that have been concentrated by our recently described short-circuit instability. We extend our work on this process by including the effects of radiative cooling, taking into account the temperature dependence of the opacity; and by examining current sheet geometry in three-dimensional, global models of magnetorotational instability. We find that temperatures above 1600 K can be reached for favorable parameters that match the ideal global models. This mechanism could provide an efficient means of tapping the gravitational potential energy of the protoplanetary disk to heat grains strongly enough to form high-temperature minerals. The volume-filling nature of turbulent magnetic reconnection is compatible with constraints from chondrule-matrix complementarity, chondrule-chondrule complementarity, the occurrence of igneous rims, and compound chondrules. The same short-circuit mechanism may perform other high-temperature mineral processing in protoplanetary disks such as the production of crystalline silicates and CAIs.Comment: 6 pages, 3 figures, ApJL published versio

Correlation between the spatial distribution of circumstellar disks and massive stars in the young open cluster NGC 6611. II: Cluster members selected with Spitzer/IRAC

Context: the observations of the proplyds in the Orion Nebula Cluster, showing clear evidence of ongoing photoevaporation, have provided a clear proof about the role of the externally induced photoevaporation in the evolution of circumstellar disks. NGC 6611 is an open cluster suitable to study disk photoevaporation, thanks to its large population of massive members and of stars with disk. In a previous work, we obtained evidence of the influence of the strong UV field generated by the massive cluster members on the evolution of disks around low-mass Pre-Main Sequence members. That work was based on a multi-band BVIJHK and X-ray catalog purposely compiled to select the cluster members with and without disk. Aims: in this paper we complete the list of candidate cluster members, using data at longer wavelengths obtained with Spitzer/IRAC, and we revisit the issue of the effects of UV radiation on the evolution of disks in NGC 6611. Methods: we select the candidate members with disks of NGC 6611, in a field of view of 33'x34' centered on the cluster, using IRAC color-color diagrams and suitable reddening-free color indices. Besides, using the X-ray data to select Class III cluster members, we estimate the disks frequency vs. the intensity of the incident radiation emitted by massive members. Results: we identify 458 candidate members with circumstellar disks, among which 146 had not been revealed in our previous work. Comparing of the various color indices we used to select the cluster members with disk, we claim that they detect the excesses due to the emission of the same physical region of the disk: the inner rim at the dust sublimation radius. Our new results confirm that UV radiation from massive stars affects the evolution of nearby circumstellar disks.Comment: Accepted for publication at Astronomy & Astrophysic

Post-translational protein deimination signatures in sea lamprey (Petromyzon marinus) plasma and plasma-extracellular vesicles

Lampreys are a jawless vertebrate species belonging to an ancient vertebrate lineage that diverged from a common ancestor with humans ~500 million years ago. The sea lamprey (Petromyzon marinus) has a filter feeding ammocoete larval stage that metamorphoses into a parasitic adult, feeding both on teleost and elasmobranch fish. Lampreys are a valuable comparative model species for vertebrate immunity and physiology due to their unique phylogenetic position, unusual adaptive immune system, and physiological adaptions such as tolerance to salinity changes and urea. Peptidylarginine deiminases (PADs) are a phylogenetically conserved enzyme family which catalyses post-translational deimination/citrullination in target proteins, enabling proteins to gain new functions (moonlighting). The identification of deiminated protein targets in species across phylogeny may provide novel insights into post-translational regulation of physiological and pathophysiological processes. Extracellular vesicles (EVs) are membrane vesicles released from cells that carry cargos of small molecules and proteins for cellular communication, involved in both normal and pathological processes. The current study identified deimination signatures in proteins of both total plasma and plasma-EVs in sea lamprey and furthermore reports the first characterisation of plasma-EVs in lamprey. EVs were poly-dispersed in the size range of 40–500 nm, similar to what is observed in other taxa, positive for CD63 and Flotillin-1. Plasma-EV morphology was confirmed by transmission electron microscopy. Assessment of deimination/citrullination signatures in lamprey plasma and plasma-EVs, revealed 72 deimination target proteins involved in immunity, metabolism and gene regulation in whole plasma, and 37 target proteins in EVs, whereof 24 were shared targets. Furthermore, the presence of deiminated histone H3, indicative of gene-regulatory mechanisms and also a marker of neutrophil extracellular trap formation (NETosis), was confirmed in lamprey plasma. Functional protein network analysis revealed some differences in KEGG and GO pathways of deiminated proteins in whole plasma compared with plasma-EVs. For example, while common STRING network clusters in plasma and plasma-EVs included Peptide chain elongation, Viral mRNA translation, Glycolysis and gluconeogenesis, STRING network clusters specific for EVs only included: Cellular response to heat stress, Muscle protein and striated muscle thin filament, Nucleosome, Protein processing in endoplasmic reticulum, Nucleosome and histone deacetylase complex. STRING network clusters specific for plasma were: Adipokinetic hormone receptor activity, Fibrinogen alpha/beta chain family, peptidase S1A, Glutathione synthesis and recycling-arginine, Fructose 1,6-bisphosphate metabolic process, Carbon metabolism and lactate dehydrogenase activity, Post-translational protein phosphorylation, Regulation of insulin-like growth factor transport and clotting cascade. Overall, for the EV citrullinome, five STRING network clusters, 10 KEGG pathways, 15 molecular GO pathways and 29 Reactome pathways were identified, compared with nine STRING network clusters, six KEGG pathways, two Molecular GO pathways and one Reactome pathway specific for whole plasma; while further pathways were shared. The reported findings indicate that major pathways relevant for immunity and metabolism are targets of deimination in lamprey plasma and plasma-EVs, with some differences, and may help elucidating roles for the conserved PAD enzyme family in regulation of immune and metabolic function throughout phylogeny

Polysaccharide Layer-by-Layer Coating for Polyimide-Based Neural Interfaces

: Implantable flexible neural interfaces (IfNIs) are capable of directly modulating signals of the central and peripheral nervous system by stimulating or recording the action potential. Despite outstanding results in acute experiments on animals and humans, their long-term biocompatibility is hampered by the effects of foreign body reactions that worsen electrical performance and cause tissue damage. We report on the fabrication of a polysaccharide nanostructured thin film as a coating of polyimide (PI)-based IfNIs. The layer-by-layer technique was used to coat the PI surface due to its versatility and ease of manufacturing. Two different LbL deposition techniques were tested and compared: dip coating and spin coating. Morphological and physiochemical characterization showed the presence of a very smooth and nanostructured thin film coating on the PI surface that remarkably enhanced surface hydrophilicity with respect to the bare PI surface for both the deposition techniques. However, spin coating offered more control over the fabrication properties, with the possibility to tune the coating's physiochemical and morphological properties. Overall, the proposed coating strategies allowed the deposition of a biocompatible nanostructured film onto the PI surface and could represent a valid tool to enhance long-term IfNI biocompatibility by improving tissue/electrode integration

Polysaccharide Layer-by-Layer Coating for Polyimide-Based Neural Interfaces

Implantable flexible neural interfaces (IfNIs) are capable of directly modulating signals of the central and peripheral nervous system by stimulating or recording the action potential. Despite outstanding results in acute experiments on animals and humans, their long-term biocompatibility is hampered by the effects of foreign body reactions that worsen electrical performance and cause tissue damage. We report on the fabrication of a polysaccharide nanostructured thin film as a coating of polyimide (PI)-based IfNIs. The layer-by-layer technique was used to coat the PI surface due to its versatility and ease of manufacturing. Two different LbL deposition techniques were tested and compared: dip coating and spin coating. Morphological and physiochemical characterization showed the presence of a very smooth and nanostructured thin film coating on the PI surface that remarkably enhanced surface hydrophilicity with respect to the bare PI surface for both the deposition techniques. However, spin coating offered more control over the fabrication properties, with the possibility to tune the coating’s physiochemical and morphological properties. Overall, the proposed coating strategies allowed the deposition of a biocompatible nanostructured film onto the PI surface and could represent a valid tool to enhance long-term IfNI biocompatibility by improving tissue/electrode integration

Disk evolution in the Ori OB1 association

We analyze multi-band photometry of a subsample of low mass stars in the associations Ori OB1a and 1b discovered during the CIDA Orion Variability Survey, which have ages of 7 - 10 Myr and 3 - 5 Myr, respectively. We obtained UBVRcIc photometry at Mt. Hopkins for 6 Classical T Tauri stars (CTTS) and 26 Weak T Tauri stars (WTTS) in Ori OB1a, and for 21 CTTS and 2 WTTS in Ori OB1b. We also obtained L band photometry for 14 CTTS at Mt. Hopkins, and 10um and 18um photometry with OSCIR at Gemini for 6 CTTS; of these, all 6 were detected at 10um while only one was detected at 18um. We estimate mass accretion rates from the excess luminosity at U, and find that they are consistent with determinations for a number of other associations, with or without high mass star formation. The observed decrease of mass accretion rate with age is qualitatively consistent with predictions of viscous evolution of accretion disks. We find an overall decrease of disk emission from Taurus to Ori OB1b to Ori OB1a. This decrease implies that significant grain growth and settling towards the midplane has taken place in the inner disks of Ori OB1. We compare the SED of the star detected at both 10um and 18um with disk models for similar stellar and accretion parameters. We find that the low <= 18 um fluxes of this Ori OB1b star cannot be due to the smaller disk radius expected from viscous evolution in the presence of the FUV radiation fields from the OB stars in the association. Instead, we find that the disk of this star is essentially a flat disk, with little if any flaring, indicating a a significant degree of dust settling towards the midplane, as expected from dust evolution in protoplanetary disks.Comment: 35 pages, 11 figures, to appear in the Astronomical Journal. Full resolution figures in http://www.cida.ve/~briceno/publications

Acute Hypoxia Alters Extracellular Vesicle Signatures and the Brain Citrullinome of Naked Mole-Rats (Heterocephalus glaber)

Peptidylarginine deiminases (PADs) and extracellular vesicles (EVs) may be indicative biomarkers of physiological and pathological status and adaptive responses, including to diseases and disorders of the central nervous system (CNS) and related to hypoxia. While these markers have been studied in hypoxia-intolerant mammals, in vivo investigations in hypoxia-tolerant species are lacking. Naked mole-rats (NMR) are among the most hypoxia-tolerant mammals and are thus a good model organism for understanding natural and beneficial adaptations to hypoxia. Thus, we aimed to reveal CNS related roles for PADs in hypoxia tolerance and identify whether circulating EV signatures may reveal a fingerprint for adaptive whole-body hypoxia responses in this species. We found that following in vivo acute hypoxia, NMR: (1) plasma-EVs were remodelled, (2) whole proteome EV cargo contained more protein hits (including citrullinated proteins) and a higher number of associated KEGG pathways relating to the total proteome of plasma-EVs Also, (3) brains had a trend for elevation in PAD1, PAD3 and PAD6 protein expression, while PAD2 and PAD4 were reduced, while (4) the brain citrullinome had a considerable increase in deiminated protein hits with hypoxia (1222 vs. 852 hits in normoxia). Our findings indicate that circulating EV signatures are modified and proteomic content is reduced in hypoxic conditions in naked mole-rats, including the circulating EV citrullinome, while the brain citrullinome is elevated and modulated in response to hypoxia. This was further reflected in elevation of some PADs in the brain tissue following acute hypoxia treatment. These findings indicate a possible selective role for PAD-isozymes in hypoxia response and tolerance

The Effects of UV Continuum and Lyman alpha Radiation on the Chemical Equilibrium of T Tauri Disks

We show in this Letter that the spectral details of the FUV radiation fields have a large impact on the chemistry of protoplanetary disks surrounding T Tauri stars. We show that the strength of a realistic stellar FUV field is significantly lower than typically assumed in chemical calculations and that the radiation field is dominated by strong line emission, most notably Lyman alpha radiation. The effects of the strong Lyman alpha emission on the chemical equilibrium in protoplanetary disks has previously been unrecognized. We discuss the impact of this radiation on molecular observations in the context of a radiative transfer model that includes both direct attenuation and scattering. In particular, Lyman alpha radiation will directly dissociate water vapor and may contribute to the observed enhancements of CN/HCN in disks.Comment: 14 pages, 4 figures, accepted by ApJ Letter