FIBER OPTICAL pH-SENSOR BASED ON LASER-INDUCED FLUORESCENCE

A fiber optical pH-sensor based on laser-induced fluorescence was developed. The fluorescence was ww. with the light of a Helium-Neon-Laser (5°mW, Polytec model PL 7504) emitting at X4 632.8 nm. The dye used was tetra-(p-sulfophenyl)-porphyrine (Ventron), ‘whieh-has in an aqueous solution an extinction coefficient at A = 632.8 nm of e = 4365 I/mol - cm. Its fluorescence behaviour changes at different-pH-values- (figure). It is a water-soluble dye and the sulfo-groups give the possibility to immobilize this dye on anion exchange resins. The fluorescence spectrum of this dye has a maximum at pH = 6.8 at A= 675 nm and A= 700 nm, respectively, which offers the advantage of a ratio-measurement

Untersuchungen an immobilisierten Porphyrinderivaten zur Entwicklung Cadmium-senitiver Schichten fuer einen optisch-chemischen Sensor

SIGLETIB: ZA 5141(4811) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

EAACI Molecular Allergology User's Guide 2.0

Since the discovery of immunoglobulin E (IgE) as a mediator of allergic diseases in 1967, our knowledge about the immunological mechanisms of IgE-mediated allergies has remarkably increased. In addition to understanding the immune response and clinical symptoms, allergy diagnosis and management depend strongly on the precise identification of the elicitors of the IgE-mediated allergic reaction. In the past four decades, innovations in bioscience and technology have facilitated the identification and production of well-defined, highly pure molecules for component-resolved diagnosis (CRD), allowing a personalized diagnosis and management of the allergic disease for individual patients. The first edition of the "EAACI Molecular Allergology User's Guide" (MAUG) in 2016 rapidly became a key reference for clinicians, scientists, and interested readers with a background in allergology, immunology, biology, and medicine. Nevertheless, the field of molecular allergology is moving fast, and after 6 years, a new EAACI Taskforce was established to provide an updated document. The Molecular Allergology User's Guide 2.0 summarizes state-of-the-art information on allergen molecules, their clinical relevance, and their application in diagnostic algorithms for clinical practice. It is designed for both, clinicians and scientists, guiding health care professionals through the overwhelming list of different allergen molecules available for testing. Further, it provides diagnostic algorithms on the clinical relevance of allergenic molecules and gives an overview of their biology, the basic mechanisms of test formats, and the application of tests to measure allergen exposure