Dendrochronological Reconstruction of Fire, Bogus Basin Area Boise National Forest

Since people have settled along the Boise Front, there have been very few documented fire disturbances. This is likely an outcome of fire suppression policies. There is evidence, visible as scars on living trees, that the old growth ponderosa pines located on the East Side Trail in the Boise National Forest have withstood multiple fires. Samples were collected by using a chainsaw to remove small, partial cross-sections from several trees. The Boise Front master chronology was used to accurately cross-date the fire-scarred samples using COFECHA. Analysis of the samples revealed ten fire events ranging from 1709 to 1889. The fire frequency during this time appeared to be between 15 and 50 years. Understanding past events may help us better understand future fires caused by increasing temperatures and drought. This climate change is projected to impact sagebrush-forest ecotone for the region

Developing a Tree-ring Chronology for Reconstructing Past Climate Conditions: Coral Pink Sand Dunes, UT

The Coral Pink Sand Dunes (CPSD) is one of the largest active dune fields in the Grand Staircase of the Colorado Plateau. A disjunct stand of ponderosa pine (Pinus ponderosa) seemingly thrives within the dune field. The presence of a multiple-aged stand suggests the site has experienced varied climatic conditions since becoming established. Other geomorphic systems in the region (e.g., arroyos) indicate sensitivity to small climate fluctuations; perhaps the dunes are similarly sensitive, leading to the question—could the trees be used to reconstruct past climate, and could that reconstruction be used to model dune activity with climate variability. The climate-growth relationship was tested by correlating the tree-ring chronology with precipitation data from a climate reporting station in Kanab, UT (15 km to the east) yielding a correlation coefficient of r = 0.73. Calibration and verification of the model results in a reconstruction of precipitation dating back to the beginning of the tree-ring record in 1775. Spectra analysis of the reconstruction determined a 20-year mode of climate variability similar to the Pacific Decadal Oscillation. Comparison between the reconstruction and teleconnection indices showed that precipitation at CPSD responds to variability in the Pacific basin. The results of this study can be applied to other studies on dune activity variability with climate, and further, how dune activity corresponds to other geomorphic processes in the region

Building Disaster Resilience: Steps toward Sustainability

Disaster losses continue to escalate globally and in many regions human losses (death, injury, permanent displacement) often exceed the economic toll. Current disaster policies are reactive with a short-term focus―respond and rebuild as quickly as possible and in the same way after the event. Such policies ignore the longer-term approach of building disaster-resilient communities, in which investments made now show financial and social returns later by reducing the impact of disasters. This article provides a vision for resilient nations in 2030 based on three recent policy reports. It highlights the necessary steps to wards achieving sustainability using the lens of disaster resilience as the pathway towards strengthening communities' ability to prepare and plan for, absorb, respond to, and recover from present and future disasters

Spatial patterns of natural hazards mortality in the United States

<p>Abstract</p> <p>Background</p> <p>Studies on natural hazard mortality are most often hazard-specific (e.g. floods, earthquakes, heat), event specific (e.g. Hurricane Katrina), or lack adequate temporal or geographic coverage. This makes it difficult to assess mortality from natural hazards in any systematic way. This paper examines the spatial patterns of natural hazard mortality at the county-level for the U.S. from 1970–2004 using a combination of geographical and epidemiological methods.</p> <p>Results</p> <p>Chronic everyday hazards such as severe weather (summer and winter) and heat account for the majority of natural hazard fatalities. The regions most prone to deaths from natural hazards are the South and intermountain west, but sub-regional county-level mortality patterns show more variability. There is a distinct urban/rural component to the county patterns as well as a coastal trend. Significant clusters of high mortality are in the lower Mississippi Valley, upper Great Plains, and Mountain West, with additional areas in west Texas, and the panhandle of Florida, Significant clusters of low mortality are in the Midwest and urbanized Northeast.</p> <p>Conclusion</p> <p>There is no consistent source of hazard mortality data, yet improvements in existing databases can produce quality data that can be incorporated into spatial epidemiological studies as demonstrated in this paper. It is important to view natural hazard mortality through a geographic lens so as to better inform the public living in such hazard prone areas, but more importantly to inform local emergency practitioners who must plan for and respond to disasters in their community.</p

Abstract 2934: GSK3β modulates chemoresistance in epithelial ovarian cancer

Introduction: Epithelial ovarian cancer is one of the most common gynecological malignancies and the fifth most frequent cause of cancer death in women, affecting over 22,000 women annually. Nearly 15,500 affected women die from this disease annually, and chemoresistance from the commonly prescribed platinum-based drug, carboplatin, is a major contributor to this mortality. Previous studies have identified genes with CpG islands that are methylated and transcriptionally silenced in resistant epithelial ovarian cancer patients. One of these genes is GSK3β, an important regulator of apoptosis and cell growth in the Wnt pathway. Thus, understanding the role of GSK3β suppression in chemoresistance of epithelial ovarian cancer can help contribute to more effective treatments for this disease. By performing different assays our study examined the functional role that GSK3β plays in carboplatin chemoresistance. Procedure: Human ovarian surface epithelium (HOSE) 6-3 cell line was utilized, which is characterized as sensitive to carboplatin therapy. The cells were studied in six groups: 1) untreated; 2) treated with Lithium Chloride (LiCl); 3) treated with carboplatin; 4) treated with carboplatin and LiCl; 5) treated with doxorubicin as control; 6) treated with doxorubicin and LiCl as another control. LiCl is known to suppress GSK3β gene expression. We took images of cells using a fluorescence microscope. We also performed the Neutral Red Dye assay that determines cell viability, Vybrant® MTT Cell Assay which measures amount of non-viable cells, Caspase 3 Assay which measures cell apoptosis, and we did cell counting using a hemocytometer and a light microscope. Data analysis was done by T-tests using Microsoft Excel®, with p \u3c .05 for significance. Summary of Data: More growth was observed in the carboplatin and LiCl group compared to the carboplatin group alone on microscopy. Neutral Red Dye assay: Compared to the cells exposed to carboplatin alone, those exposed to carboplatin and LiCl were more viable (p \u3c0.01). Vybrant® MTT Cell Assay: the cells treated with carboplatin and LiCl showed lesser amounts of nonviable cells as compared to the carboplatin alone group (p\u3c0.01). Caspase 3 Assay: the cells treated with carboplatin and LiCl were less apoptotic, compared to cells treated with carboplatin alone (p \u3c0.01). Cell Counting: cells treated with carboplatin and LiCl had significantly more growth compared to the cells treated with carboplatin alone (p \u3c0.01). Conclusion: Our results show that cells with suppressed GSK3β had increased proliferation and reduced apoptosis, strongly suggesting that silenced GSK3β expression contributes to carboplatin resistance and GSK3β expression is vital to carboplatin chemosensitivity. Future in vivo studies could further investigate the role of GSK3β methylation to facilitate the design of potential genome-guided treatments for patients with chemoresistant epithelial ovarian cancer

Evaluating the Biogeochemical Cycle of Selenium in San Francisco Bay Through Modeling

A biogeochemical model was developed to simulate salinity, total suspended material, phytoplankton biomass, dissolved selenium concentrations (selenite, selenate, and organic selenide), and particulate selenium concentrations (selenite + selenate, elemental selenium, and organic selenide) in the San Francisco Bay estuary. Model-generated estuarine profiles of total dissolved selenium reproduced observed estuarine profiles at a confidence interval of 91- 99% for 8 different years under various environmental conditions. The model accurately reproduced the observed dissolved speciation at confidence intervals of 81-98% for selenite, 72-91% for selenate, and 60-96% for organic selenide. For particulate selenium, model-simulated estuarine profiles duplicated the observed behavior of total particulate selenium (76-93%), elemental selenium (80-97%), selenite + selenate (77-82%), and organic selenide (70-83%). Discrepancies between model simulations and the observed data provided insights into the estuarine biogeochemical cycle of selenium that were largely unknown (e.g., adsorption/desorption). Forecasting simulations investigated how an increase in the discharge from the San Joaquin River and varying refinery inputs affect total dissolved and particulate selenium within the estuary. These model runs indicate that during high river flows the refinery signal is undetectable, but when river flow is low (70- day residence time) total particle-associated selenium concentrations can increase to \u3e2 µg g-1 . Increasing the San Joaquin River discharge could also increase the total particle-associated selenium concentrations to \u3e1 µg g-1 . For both forecasting simulations, particle-associated selenium was predicted to be higher than current conditions and reached levels where selenium could accumulate in the estuarine food web

Research Notes: Histology of the Embryo Sac of Male Sterile ms1ms1 Soybeans

The fact that ms1ms1 plants in maturity ranges I to V were producing haploids, triploids, and even higher ploidy levels along with the predominant normal diploids, indicated the female gametophyte was at least occasionally functioning abnormally. Histological sections of 92 male sterile pistils from plants about Groups III and IV, indicated only about 28% of the ovules had a normal embryo sac, by our interpretation. The remainder most commonly had extra nuclei in the regions of the secondary nucleus (endosperm mother cell) and/or the egg apparatus

Changes in Breast Cancer Care in New York During the COVID-19 Pandemic

Breast cancer is the second most common malignancy among women in the United States. As such, the COVID-19 pandemic has caused medical facilities to change their methods of operation since March of 2020, including changes in diagnosis and treatment plans. New York (NY) has an unusually high incidence of breast cancer. This study analyzed the effects of the COVID-19 pandemic on breast cancer care (BCC) in NY. Women in NY that were diagnosed with or in remission for breast cancer were asked to take an online, anonymous survey regarding their BCC experience. For patients in treatment, 26% of women wished they had greater emotional support or had a family member included in their appointments. 39% of women do not feel they are receiving as good of care as before, while 31% feel they are receiving the same level of care. Additionally, 41% of women feel they received the same level of care over telemedicine. Our data show a negative correlation between the quality of care received during the pandemic and the wish for more emotional support and inclusion of supportive members in the care process (nonsignificant). There was less of a negative correlation between the quality of telemedicine care received during the pandemic and the wish for more emotional support and inclusion of supportive members in the care process (nonsignificant). This indicates that most women lacking emotional support reported worse BCC experiences, and telemedicine use was not as troublesome to patients as the lack of emotional support. Most women in treatment and in remission reported negative feelings like fear when asked about their BCC experiences. Our data show the importance of emotional support for breast cancer patients and those in remission during the COVID-19 pandemic. Our work could also provide clinicians with the knowledge necessary on how breast cancer care should be handled in an evolving pandemic such as COVID-19

Inhibitions of GSK3β Modulates Cell Death in Epithelial Ovarian Cancer

Epithelial ovarian cancer is one of the most common gynecological malignancies and the fifth most frequent cause of cancer death in women, affecting over 22,000 women annually. Nearly 15,500 affected women die from this disease annually, and chemoresistance from the commonly prescribed platinum-based drug, carboplatin, is a major contributor to this mortality rate. Previous studies have identified genes with CpG islands that are methylated and transcriptionally silenced in resistant epithelial ovarian cancer patients. One of these genes is GSK3β, an important regulator of apoptosis and cell growth in the Wnt pathway. Thus, understanding the role of GSK3β suppression in chemoresistance of epithelial ovarian cancer can help contribute to more effective treatments for this disease. By performing assays of cell growth, viability, and apoptosis, our study examined the functional role that GSK3β plays in carboplatin mediated apoptosis. Our results suggest that cells with suppressed GSK3β had increased proliferation and reduced apoptosis. We conclude that silenced GSK3β expression might therefore contribute to carboplatin resistance seen in tumors and our in vitro analysis suggests that GSK3β expression is vital to carboplatin chemosensitivity. Future research is required to further investigate the role of GSK3β methylation to facilitate the design of potential genome-guided treatments for patients with chemoresistant epithelial ovarian cancer

Improved antimicrobial activity of nisin-incorporated polymer films by formulation change and addition of food grade chelator

Aims: The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). Methods and Results: Of the polymers tested, PE and PE + PEO did not exhibit any antimicrobial activity against Brochothrix thermosphacta (BT); however, PE + nisin, PE + nisin + EDTA, and PE + PEO + nisin did. Beef surfaces were experimentally inoculated with 3·50 log10 cfu/cm2 of BT, vacuum packaged with each of the five polymers, and held at 4°C for 21 d. After 3 d at 4°C, BT was reduced \u3e 1·70 log10 by PE + nisin and \u3e 3·50 log10 with PE + nisin + EDTA or PE + PEO + nisin. By 21 d at 4°C, BT was reduced to 0·30 log10 cfu/cm2 when treated with PE + PEO + nisin. Conclusions: It appears that PE + PEO + nisin or PE + nisin + EDTA were more effective for reducing BT, as compared to polymers composed of PE + nisin. Significance and Impact of the Study: Nisin-incorporated polymers may control the growth of undesirable bacteria, thereby extending the shelf life and possibly enhancing the microbial safety of meats