New life to Italian university anatomical collections: desire to give value and open museological issues. Cases compared

The anatomical museums are one of the most difficult categories of museums to deal with because the issues addressed and the stored materials are complex to communicate and often not suitable for all audiences. The history of medicine teaches us that the knowledge of our body is a fascinating topic that continues to be the subject of study and research. The Italian anatomical museums are mostly university property, often closed and with specimens in urgent need of restoration. Their rooms still house important collections of human biological samples, dry or in liquid, collected between the eighteenth and twentieth century: a historical heritage that testifies to the evolution of medical science and provides a searchable archive of biological and genetic data. The curator of such a museum must confront many issues \u2013 museological, legislative and ethical \u2013 many of which are unclear and incomplete. This article provides an overview of museological issues in the anatomical area in order to offer ideas and visions, from a comparison of three different examples: the Museum of Human Anatomy of the University of Pavia, the Museum of Pathological Anatomy at the University of Padua and the Gordon Museum of Pathology in London

3D bioprinting and Rigenera® micrografting technology: A possible countermeasure for wound healing in spaceflight

Plant and animal life forms have progressively developed mechanisms for perceiving and responding to gravity on Earth, where homeostatic mechanisms require feedback. Lack of gravity, as in the International Space Station (ISS), induces acute intra-generational changes in the quality of life. These include reduced bone calcium levels and muscle tone, provoking skin deterioration. All these problems reduce the work efficiency and quality of life of humans not only during exposure to microgravity (mu G) but also after returning to Earth. This article discusses forthcoming experiments required under gravity and mu G conditions to ensure effective and successful medical treatments for astronauts during long-term space missions, where healthcare is difficult and not guaranteed

Skeletal Myogenic Progenitors Originating from Embryonic Dorsal Aorta Coexpress Endothelial and Myogenic Markers and Contribute to Postnatal Muscle Growth and Regeneration

Skeletal muscle in vertebrates is derived from somites, epithelial structures of the paraxial mesoderm, yet many unrelated reports describe the occasional appearance of myogenic cells from tissues of nonsomite origin, suggesting either transdifferentiation or the persistence of a multipotent progenitor. Here, we show that clonable skeletal myogenic cells are present in the embryonic dorsal aorta of mouse embryos. This finding is based on a detailed clonal analysis of different tissue anlagen at various developmental stages. In vitro, these myogenic cells show the same morphology as satellite cells derived from adult skeletal muscle, and express a number of myogenic and endothelial markers. Surprisingly, the latter are also expressed by adult satellite cells. Furthermore, it is possible to clone myogenic cells from limbs of mutant c-Met-/- embryos, which lack appendicular muscles, but have a normal vascular system. Upon transplantation, aorta-derived myogenic cells participate in postnatal muscle growth and regeneration, and fuse with resident satellite cells. The potential of the vascular system to generate skeletal muscle cells may explain observations of nonsomite skeletal myogenesis and raises the possibility that a subset of satellite cells may derive from the vascular system

Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

<p>Abstract</p> <p>Background</p> <p>Bacterial cell lysis is a widely studied mechanism that can be achieved through the intracellular expression of phage native lytic proteins. This mechanism can be exploited for programmed cell death and for gentle cell disruption to release recombinant proteins when <it>in vivo </it>secretion is not feasible. Several genetic parts for cell lysis have been developed and their quantitative characterization is an essential step to enable the engineering of synthetic lytic systems with predictable behavior.</p> <p>Results</p> <p>Here, a BioBrick™ lysis device present in the Registry of Standard Biological Parts has been quantitatively characterized. Its activity has been measured in <it>E. coli </it>by assembling the device under the control of a well characterized N-3-oxohexanoyl-L-homoserine lactone (HSL) -inducible promoter and the transfer function, lysis dynamics, protein release capability and genotypic and phenotypic stability of the device have been evaluated. Finally, its modularity was tested by assembling the device to a different inducible promoter, which can be triggered by heat induction.</p> <p>Conclusions</p> <p>The studied device is suitable for recombinant protein release as 96% of the total amount of the intracellular proteins was successfully released into the medium. Furthermore, it has been shown that the device can be assembled to different input devices to trigger cell lysis in response to a user-defined signal. For this reason, this lysis device can be a useful tool for the rational design and construction of complex synthetic biological systems composed by biological parts with known and well characterized function. Conversely, the onset of mutants makes this device unsuitable for the programmed cell death of a bacterial population.</p

A standard vector for the chromosomal integration and characterization of BioBrick™ parts in Escherichia coli

BACKGROUND: The chromosomal integration of biological parts in the host genome enables the engineering of plasmid-free stable strains with single-copy insertions of the desired gene networks. Although different integrative vectors were proposed, no standard pre-assembled genetic tool is available to carry out this task. Synthetic biology concepts can contribute to the development of standardized and user friendly solutions to easily produce engineered strains and to rapidly characterize the desired genetic parts in single-copy context. RESULTS: In this work we report the design of a novel integrative vector that allows the genomic integration of biological parts compatible with the RFC10, RFC23 and RFC12 BioBrick™ standards in Escherichia coli. It can also be specialized by using BioBrick™ parts to target the desired integration site in the host genome. The usefulness of this vector has been demonstrated by integrating a set of BioBrick™ devices in two different loci of the E. coli chromosome and by characterizing their activity in single-copy. Construct stability has also been evaluated and compared with plasmid-borne solutions. CONCLUSIONS: Physical modularity of biological parts has been successfully applied to construct a ready-to-engineer BioBrick™ vector, suitable for a stable chromosomal insertion of standard parts via the desired recombination method, i.e. the bacteriophage integration mechanism or homologous recombination. In contrast with previously proposed solutions, it is a pre-assembled vector containing properly-placed restriction sites for the direct transfer of various formats of BioBrick™ parts. This vector can facilitate the characterization of parts avoiding copy number artefacts and the construction of antibiotic resistance-free engineered microbes, suitable for industrial use

Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite

Bone graft substitutes and cancellous biomaterials have been widely used to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, porous hydroxyapatite is widely used in reconstructive bone surgery owing to its biocompatibility. In addition, the in vitro modification of cancellous hydroxyapatite with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study, we have followed a tissue-engineering strategy where ultrasonically stimulated SAOS-2 human osteoblasts proliferated and built their extracellular matrix inside a porous hydroxyapatite scaffold. The ultrasonic stimulus had the following parameters: average power equal to 149 mW and frequency of 1.5 MHz. In comparison with control conditions, the ultrasonic stimulus increased the cell proliferation and the surface coating with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The mechanical stimulus aimed at obtaining a better modification of the biomaterial internal surface in terms of cell colonization and coating with bone matrix. The modified biomaterial could be used, in clinical applications, as an implant for bone repair

Evaluation of the effects of specific karate exercises during multilateral training in children of primary school

The early specialization in the development of sport skills is a point of discussion among researchers, even if the general trend is to encourage multilateral activities in children. The aim of this study was to evaluate the effect of specific karate exercises added during a program of multilateral exercises in a group of school age children. A sample of 82 primary school children (39 females, 6.4 ± 0.3 y and 43 males, 6.3 ± 0.3 y) were randomly assigned to two groups: Multilateral (MG) and Special (SG). MG was composed of 19 females (MGf, 6,4 ± 0,3 y) and 22 males (MGm, 6,3 ± 0,3 y), while SG was composed of 20 females (SGf, 6,3 ± 0,3 y) and 21 males (SGm, 6,4 ± 0,3 y). During the training period of eight weeks, the MG group has played only multilateral activities, while the SG group has also done specific exercises of Karate. At the end of the training period both groups were subjected to some physical evaluation test and the results was statistically analyzed (ANOVA). Although both groups (Mg and SG) have improved significantly (p &lt; 0.05) compared to the initial stage, the comparison between the two groups (MG vs SG) has not revealed significant differences in relation to the considered motor skills (speed, agility, strength, coordination), with the exception of the ability of static balance, in which the SG group showed a significant improvement compared to the MG group (p = 0.019). In particular, the improvement appears to be due mainly to the female component (SGf vs MGf: p = 0,012; SGm vs MGm p = 0,20). The fact that the improvement was mainly dependent on the female group deserves future investigations The results seem to confirm the fact that the multilateral activities would be sufficient to improve motor skills in primary school children, although some neuromotor abilities could be improved through more specific exercises without creating particular damag

Safe use of human anatomical preparations in frontal and interactive teaching

In the institute of Human Anatomy of Pavia, the use of cadaver dissection is not economically feasible. In order to improve students’ preparation related to topography of the central nervous system, we decided to use formalin-fixed brains and cranial sections belonging to the collection of cadaveric specimens. These specimens, preserved in formalin, however cannot be manipulated as such by the students because formalin can cause headaches, burning sensation in the throat, difficult breathing and can trigger or aggravate asthma symptoms [1, 2]. Furthermore, formalin is known to be a human carcinogen [3]. In order to minimize toxic effects, whole brains were extensively washed in running water and then sliced according to different reference planes using a “home-made” device enabling cuts according to parallel planes. Finally, the resulting sections were inserted into transparent plastic envelopes, immerged in a solution composed by 0.5% agar and 1% sodium azide as preservative. Medical students can now use these human brain sections to test their own ability to recognize nervous system structures. This strategy optimize specimen’s choice and focalize student’s attention on peculiar, selected human samples in full compliance with current security laws

Autologous Periosteum-Derived Micrografts and PLGA/HA Enhance the Bone Formation in Sinus Lift Augmentation

Sinus lift augmentation is a procedure required for the placement of a dental implant, whose success can be limited by the quantity or quality of available bone. To this purpose, the first aim of the current study was to evaluate the ability of autologous periosteum-derived micrografts and Poly(lactic-co-glycolic acid) (PLGA) supplemented with hydroxyl apatite (HA) to induce bone augmentation in the sinus lift procedure. Secondly, we compared the micrograft's behavior with respect to biomaterial alone, including Bio-Oss® and PLGA/HA, commercially named Alos. Sinus lift procedure was performed on 24 patients who required dental implants and who, according to the study design and procedure performed, were divided into three groups: group A (Alos + periosteum-derived micrografts); group B (Alos alone); and group C (Bio-Oss® alone). Briefly, in group A, a small piece of periosteum was collected from each patient and mechanically disaggregated by Rigenera® protocol using the Rigeneracons medical device. This protocol allowed for the obtainment of autologous micrografts, which in turn were used to soak the Alos scaffold. At 6 months after the sinus lift procedure and before the installation of dental implants, histological and radiographic evaluations in all three groups were performed. In group A, where sinus lift augmentation was performed using periosteum-derived micrografts and Alos, the bone regeneration was much faster than in the control groups where it was performed with Alos or Bio-Oss® alone (groups B and C, respectively). In addition, the radiographic evaluation in the patients of group A showed a radio-opacity after 4 months, while after 6 months, the prosthetic rehabilitation was improved and was maintained after 2 years post-surgery. In summary, we report on the efficacy of periosteum-derived micrografts and Alos to augment sinus lift in patients requiring dental implants. This efficacy is supported by an increased percentage of vital mineralized tisssue in the group treated with both periosteum-derived micrografts and Alos, with respect to the control group of Alos or Bio-Oss® alone, as confirmed by histological analysis and radiographic evaluations at 6 months from treatment