4Pi Nanoscopy parallelized by line scanning

A superresolution scanning fluorescence microscope – or nanoscope – allows imaging structures smaller than the diffraction limit of light. The principal idea in targeted readout microscopy methods is the on/off-switching of a subset of fluorophores inside the focal volume with the help of different reversible switchable optical fluorescence transitions. If, in addition, the effective numerical aperture is doubled as in a 4Pi optical setup, the resolving power in axial direction of a far field microscope can be significantly increased. Due to the local field enhancement, a 4Pi augmented STimulated Emission Depletion (STED) setup exploits STED light in an effective manner. To this date, it features the smallest effective scannning volume. However, the acquisition of the obtained image information with this slender volume requires smaller scanning steps and in consequence a longer overall acquisition process. This disadvantage can be compensated for by parallel readout and is relevant for a 4Pi-STED combination, in particular. The proposed image parallelization scheme allows to trade-off acquisition speed against required resolution. In this thesis, a 4Pi augmented line parallelized STED setup is presented that is, for the first time, able to resolve 20 nm fluorescent microspheres with a resolution of about 50 nm in two dimensions with a focal volume that is 10 times parallelized as compared to a single spot confocal volume

Coral reef fish larvae show no evidence for map-based navigation after physical displacement

Millions of minute, newly hatched coral reef fish larvae get carried into the open ocean by highly complex and variable currents. To survive, they must return to a suitable reef habitat within a species-specific time. Strikingly, previous studies have demonstrated that return to home reefs is much more frequent than would be expected by chance. It has been shown that magnetic and sun compass orientation can help cardinalfish maintain their innate swimming direction but do they also have a navigational map to cope with unexpected displacements? If displaced settling-stage cardinalfish Ostorhinchus doederleini use positional information during their pelagic dispersal, we would expect them to re-orient toward their home reef. However, after physical displacement by 180 km, the fish showed a swimming direction indistinguishable from original directions near the capture site. This suggests that the tested fish rely on innate or learned compass directions and show no evidence for map-based navigation

Light Availability Affects the Symbiosis of Sponge Specific Cyanobacteria and the Common Blue Aquarium Sponge (Lendenfeldia chondrodes)

Bacterial symbionts in marine sponges play a decisive role in the biological and ecological functioning of their hosts. Although this topic has been the focus of numerous studies, data from experiments under controlled conditions are rare. To analyze the ongoing metabolic processes, we investigated the symbiosis of the sponge specific cyanobacterium Synechococcus spongiarum and its sponge host Lendenfeldia chondrodes under varying light conditions in a defined aquarium setting for 68 days. Sponge clonal pieces were kept at four different light intensities, ranging from no light to higher intensities that were assumed to trigger light stress. Growth as a measure of host performance and photosynthetic yield as a proxy of symbiont photosynthetic activity were measured throughout the experiment. The lack of light prevented sponge growth and induced the expulsion of all cyanobacteria and related pigments by the end of the experiment. Higher light conditions allowed rapid sponge growth and high cyanobacteria densities. In addition, photosynthetically active radiation above a certain level triggered an increase in cyanobacteria’s lutein levels, a UV absorbing protein, thus protecting itself and the host’s cells from UV radiation damage. Thus, L. chondrodes seems to benefit strongly from hosting the cyanbacterium S. spongiarum and the relationship should be considered obligatory mutualistic

Search Strategy Analysis of 5xFAD Alzheimer Mice in the Morris Water Maze Reveals Sex- and Age-Specific Spatial Navigation Deficits

Spatial disorientation and navigational impairments are not only some of the first memory deficits in Alzheimer’s disease, but are also very disease-specific. In rodents, the Morris Water Maze is used to investigate spatial navigation and memory. Here, we examined the spatial memory in the commonly used 5xFAD Alzheimer mouse model in a sex- and age-dependent manner. Our findings show first spatial learning deficits in 7-month-old female 5xFAD and 12-month-old male 5xFAD mice, respectively. While the assessment of spatial working memory using escape latencies provides a global picture of memory performance, it does not explain how an animal solves a spatial task. Therefore, a detailed analysis of swimming strategies was performed to better understand the behavioral differences between 5xFAD and WT mice. 5xFAD mice used a qualitatively and quantitatively different search strategy pattern than wildtype animals that used more non-spatial strategies and showed allocentric-specific memory deficits. Furthermore, a detailed analysis of swimming strategies revealed allocentric memory deficits in the probe trial in female 3-month-old and male 7-month-old 5xFAD animals before the onset of severe reference memory deficits. Overall, we could demonstrate that spatial navigation deficits in 5xFAD mice are age- and sex-dependent, with female mice being more severely affected. In addition, the implementation of a search strategy classification system allowed an earlier detection of behavioral differences and therefore could be a powerful tool for preclinical drug testing in the 5xFAD model

Beachrock-type calcarenitic tsunamites along the shores of the eastern Ionian Sea (western Greece) - case studies from Akarnania, the Ionian Islands and the western Peloponnese

This paper presents geo-scientific evidence of beachrock-type calcarenitic tsunamites from three study areas in western Greece, namely from the Bays of Aghios Nikolaos (Akarnania), Langadakia (Cefalonia Island) and Aghios Andreas (Peloponnese). Geomorphological, sedimentological, micromorphological and geochemical studies were conducted to clarify depositional processes and the post-sedimentary evolution. Calcarenitic and locally conglomeratic carbonate crusts were studied in natural outcrops along the seafront and in vibracores. High-resolution topographic surveys and 3D-visualisation were carried out by differential GPS and LIDAR measurements. Tsunami impact was dated by a combined approach of radiocarbon, OSL and archaeological age determination and compared to local tsunami and earthquake chronologies. We found sedimentary structures such as basal unconformities, rip-up and intra-clasts, evidence of fining upward, thinning landward and upward increase in sorting as well as bi-to multimodal deposits and injection structures all of which are described as features typical of recent or historic tsunami deposits. Typically non-littoral sedimentary features such as load casts and convolute bedding further indicate gravity driven processes in water-saturated sheets of allochthonous deposits and are well known from, for example, turbidites. Moreover, thin section analyses revealed high-energy shock- and impact-borne cracking and shearing effects. Our results show that cementation of tsunami deposits may occur by post-depositional pedogenetic decalcification of higher sections and subsequent secondary carbonate precipitation in lower sections of tsunami deposits provided that they were deposited above sea level. The calcarenitic tsunamites encountered in the three study areas match the definition of beachrock sensu stricto. This is thus the first paper giving examples of beachrock sequences that are interpreted as partially cemented tsunami deposits. Consequently, beachrock is recommended not to be used as sea level indicator in future studies unless a tsunami-genic formation can be definitely excluded. Dating results brought to light young, mostly Holocene ages of tsunami sediments. In the Bay of Aghios Andreas, western Peloponnese, we found spectacular traces that Olympia’s ancient harbour site Pheia was destroyed by tsunami impact in the 66 cent. AD and covered by a rapidly cemented, up to 3 m-thick beachrock-type tsunami deposit