AKT Hyperphosphorylation and T Cell Exhaustion in Down Syndrome

Down syndrome (DS) is associated with increased susceptibility to infections, auto-immunity, immunodeficiency and haematological malignancies. The exact underlying immunological pathophysiology is still unclear. The immunophenotype and clinical characteristics of DS resemble those of Activated PI3K Delta Syndrome (APDS), in which the PI3K/AKT/mTOR pathway is overactivated. We hypothesized that T cell exhaustion and the hyperactivation of the AKT signalling pathway is also present in immune cells of children with DS. In this observational non-interventional cohort study we collected blood samples of children with DS (n=22) and healthy age-matched controls (n=21) for flowcytometric immunophenotyping, phospho-flow AKT analysis and exhaustion analysis of T cells. The median age was 5 years (range 1-12y). Total T and NK cells were similar for both groups, but absolute values and transitional B cells, naive memory B cells and naive CD4+ and CD8+ T cells were lower in DS. pAKT and AKT were increased for CD3+ and CD4+ T cells and CD20+ B cells in children with DS. Total AKT was also increased in CD8+ T cells. Children with DS showed increased expression of inhibitory markers Programmed cell dealth-1 (PD-1), CD244 and CD160 on CD8+ T cells and increased PD-1 and CD244+ expression on CD4+ T cells, suggesting T cell exhaustion. Children with DS show increased pAKT and AKT and increased T cell exhaustion, which might contribute to their increased susceptibility to infections, auto immunity and haematological malignancies

Influence of killing agents and water on the capture of six Coleoptera species in probe traps

The introduction of propylene glycol, DDVP and water inside perforated probe traps was evaluated on the basis of their effectiveness in trapping adults of six species of Coleoptera infesting stored cereals. Adults of Sitophilus oryzae, Tribolium castaneum, Cryptolestes ferrugineus, Tenebroides mauritanicus, Rhyzopertha dominica and Oryzaephilus surinamensis were placed in plastic cylindrical bins each containing 14 kg of wheat. One probe trap was then introduced into each container and the adults trapped were counted 72 h later. Experiments were conducted at two population densities, of 30 adults (approx. one insect per 0.5 kg of wheat) and 90 adults (approx. three adults per 0.5 kg of wheat) of each species per container. The highest percentage of adults trapped was observed for C. ferrugineus and T. castaneum, and the lowest for R. dominica and S. oryzae. No significant differences in number of adults captured (% of the total number of adults) was noted between the two population density levels. At the lower density, the presence of killing agents in the traps did not increase the captures significantly; on the other hand, at the higher density, the killing agents helped to increase significantly the catches of S. oryzae, T castaneum, T mauritanicus and O. surinamensis, as compared with the catches in the control traps. No significant differences were noted for C. ferrugineus and R. dominica

Distribution and migration of insects and mites in flat storage containing wheat

Studies were conducted in a flat storeroom in central Greece, filled with approx., 90 tons of wheat, in order to evaluate insect and mite species abundance, and changes in population density and distribution among sampling units and locations. The surface of the grain bulk wag divided into two sampling zones: the central and the peripheral (edge). At 10-day intervals, five wheat samples were taken from the central zone and eight from the peripheral zone, with a non-partitioned grain trier (1.6-m length, 250-g capacity). Fifteen insect species and 12 mite taxa were found during the sampling period, (June 1999-Feb. 2000). The most abundant insect species were Sitophilus oryzae (L.), Cryptolestes ferriegineus (Stephens), Tribolium castaneum (Herbst) and Latheticus oryzae Waterhouse; the most abundant mite species were Acarus siro L., Lepidoglyphus destructor (Shrank), and the predators Blattisocius tarsalis (Berlese) and B. keegani Fox, Grain temperature decreased during the storage period, more slowly in the central than peripheral zone; grain moisture content increased. The highest population densities for both insects and mites were recorded during September and October. The most numerous species of insects and mites showed an aggregated spatial pattern, as indicated by Iwao's Patchiness Regression