ASSOCIATION BETWEEN CARDIORESPIRATORY FITNESS AND PHYSICAL ACTIVITY WITH SLEEP METRICS IN APPARENTLY HEALTHY ADULTS

BACKGROUND: High cardiorespiratory fitness (CRF), healthy physical activity (e.g., taking \u3e10,000 steps per day) and sleep habits (e.g., sleeping 7-9 hours per night) decrease the risk of all cause mortality. However, the association between CRF and physical activity with sleep are understudied and inconclusive. Thus, we sought to examine the associations between CRF and physical activity with objective and subjective sleep metrics. METHODS: Thirty-two adults (14 female, age 40 ± 18 years, body mass index [BMI] 25.8 ± 6.2 kg/m2) participated in this study. We assessed CRF using a graded treadmill exercise test to volitional exhaustion to determine relative VO2peak (mL/kg/min). We assessed physical activity using waist worn ActiGraph GT3X accelerometers for a minimum of 5-days (11.8 ± 3.6 days) to obtain average daily steps and moderate and vigorous physical activity (MVPA). Participants’ self-reported sleep quality was assessed using the Pittsburg Sleep Quality Index (PSQI) scored 0 [better] to 21 [worse]. Objective nightly sleep duration (hours) and efficiency (% of time in bed spent sleeping) were measured using Phillips Actiwatch Spectrum PLUS accelerometers (worn on the wrist) for a minimum of 5-days (7.1± 0.5 days days). All variables were tested for normality using the Shapiro Wilk test. We used Pearson’s r and Spearman’s rho correlations controlled for age, sex and BMI to examine associations between CRF and physical activity with sleep quality variables. Significance was set as p ≤ 0.05. RESULTS: After controlling for age, sex and BMI there was an association between VO2peak and sleep duration (r = 0.428, p = 0.021), but not PSQI (rho = -0.202, p = 0.313), or sleep efficiency (r = 0.168, p = 0.384). MVPA was not associated with PSQI (rho = -0.207, p = 0.395), sleep duration (r = 0.301, p = 0.185), or sleep efficiency (r = 0.222, p = 0.333). Steps were also not associated with PSQI (rho = -0.230, p = 0.344), sleep duration (r = 0.298, p = 0.189), or sleep efficiency (r = 0.258, p = 0.258). CONCLUSIONS: Our preliminary data indicate there is an association between cardiorespiratory fitness and objectively measured sleep duration, but not sleep efficiency, or subjective sleep quality. Physical activity was not associated with sleep. Additional data are needed to determine directionality between cardiorespiratory fitness, sleep duration, and potential mechanisms

AGE AND PHYSICAL ACTIVITY DO NOT IMPACT CIRCULATING SOLUBLE CD14 CONCENTRATION IN APPARENTLY HEALTHY ADULTS

BACKGROUND: Soluble CD14 (sCD14) (cluster of differentiation 14) is a co-receptor of bacterial lipopolysaccharide that is released from monocytes upon activation. There is a well-recognized role of sCD14 in inflammation, and aging has been associated with increased inflammation and cardiometabolic health risk factors (e.g., high blood pressure [BP], hyperglycemia). However, there is limited information on the combined influence of aging and physical activity on circulating sCD14. Therefore, the purpose of this study was to examine the influence of aging on plasma sCD14 concentration and the potential influence of physical activity. METHODS: Twenty young (11 females, age 22.7± 2.6, body mass index 26.7 ± 3.3, BP 119/73 ± 7/8 mmHg) and 21 old (11 female, age 58.4 ± 7.4, body mass index 27.8 ± 4.8, BP 125/76 ± 12/8 mmHg) adults participated in the study. Physical activity (PA) was assessed using waist worn ActiGraph GT3X accelerometers for a minimum of 5-days (7.6 ± 1.6 days) to obtain average daily steps, sedentary time, and moderate and vigorous PA (MVPA). We measured brachial BP using a SpyghmoCor XCEL after 10-minutes of supine rest. We assessed plasma concentrations of sCD14 using an enzyme-linked immunosorbent assay kit. Normality was assessed using Shapiro-Wilk. Students’ T Test or Mann Whitney test were used to make age comparisons between young (\u3c35 years) and older (\u3e45 years) adults. Pearson’s correlation and Spearman’s rho, controlled for age, body mass index, and sex, were used to assess relations between MVPA and steps with sCD14. Statistical significance was set as p ≤ 0.05 RESULTS: There was not a difference between young and older adults in circulating sCD14 concentration (young: 2348 ± 441 vs. older: 2487 ± 541 pg/ml, p = 0.501). There was not a difference between young and older adults in MVPA (young: 47 ± 25 vs. older: 48 ± 24 min/day, p = 0.873) or average daily steps (young: 7179 ± 3171 vs. older: 7797 ± 3595 steps/day, p = 0.679). There were not associations between sCD14 and daily MVPA (r = -0.176, p = 0.343) or Steps (rho = -0.278, p = 0.130). CONCLUSION: Our preliminary data indicate that there were no age differences in circulating sCD14 and no associations between habitual physical activity and circulating sCD14

AGE AND PHYSICAL ACTIVITY DOES NOT IMPACT LIPOPOLYSACCHARIDE-BINDING PROTEIN CONCENTRATION IN APPARENTLY HEALTHY ADULTS

BACKGROUND: LBP (lipopolysaccharide‐binding protein) is an acute‐phase protein that binds lipopolysaccharide in the blood and plays a role in subsequent activation of immune cells through the production of proinflammatory cytokines. LBP is associated with future cardiovascular disease in middle-aged and older adults. However, there is limited information on the combined influence of aging and physical activity on circulating LBP. Therefore, the purpose of this study was to examine the influence of aging on plasma LBP concentration and the potential influence of physical activity. METHODS: Ten young (4 females, age 23.5 ± 2.2, body mass index 26.3 ± 2.5 blood pressure 120/74 ± 8/8 mmHg) and 11 old (4 female, age 60.7 ± 5.5, body mass index 27.2 ± 3.2, blood pressure 124/74 ± 12/6 mmHg) participated in the study. Physical activity (PA) was assessed using waist worn ActiGraph GT3X accelerometers for a minimum of 6-days (7.1 ± 0.5 days) to obtain average daily steps, sedentary time, and moderate and vigorous PA (MVPA). We measured brachial blood pressure using a SpyghmoCor XCEL after 10-minutes of supine rest. We assessed plasma concentrations of LBP using an enzyme-linked immunosorbent assay kit. Normality was assessed using Shapiro-Wilk. Students’ T Test or Mann Whitney test were used to make age comparisons between young (\u3c35 years) and older (\u3e45 years) adults. Pearson’s correlation, controlled for age, body mass index, and sex, was used to assess relations between MVPA and steps with LBP. Statistical significance was set as p ≤ 0.05 RESULTS: There was not a difference between young and older adults in circulating LBP concentration (young: 8045 ± 3222 vs. older: 8905 ± 7087 pg/ml, p = 0.605). There was not a difference between young and older adults in MVPA (young: 56 ± 30 vs. older: 51 ± 28 min/day, p = 0.748) or average daily steps (young: 8150 ± 4315 vs. older: 8358 ± 4158 steps/day, p = 0.921). There were not associations between LBP and daily MVPA (r = -0.188, p = 0.519) or Steps (r = -0.267, p = 0.356). CONCLUSION: Our preliminary data indicate that there were no age differences in circulating LBP and no associations between habitual physical activity and circulating LBP