Shear strength of cemented sand gravel and rock materials

Shear strength is currently a significant parameter in the design of cemented sand gravel and rock (CSGR) dams. Shear strength tests were carried out to compare material without layers noumenon and layer condition. The experimental results showed good linearity in the curves of shear strength and pure grinding tests with correlation coefficients of nearly 97%. The friction coefficient was similar to that of C10 roller-compacted concrete (RCC), but the cohesion value was weaker than that of RCC. The shear strength of the CSGR layers decreased by 40% when retarding mixtures were not added and the layer was paved immediately after 4 h of waiting interval

The Neurogenic Compound P7C3 Regulates the Aerobic Glycolysis by Targeting Phosphoglycerate Kinase 1 in Glioma

BackgroundP7C3 is a neurogenic compound that exhibits neuroprotective properties in neural cells. However, its target proteins and effects in glioma are unknown.MethodsThe candidate P7C3 target proteins were analyzed using a human protein microarray containing 23136 human proteins. A streptavidin agarose affinity assay was used to verify the direct interaction between P7C3 and phosphoglycerate kinase 1 (PGK1). Mass spectrometry was used to identify the binding sites of PGK1 for P7C3 binding. Seahorse XF96 extracellular flux analyzer was used to measure the cell oxygen consumption rate and extracellular acidification rate. Glycolytic metabolites were measured using the related kits. Protein level was detected by western blotting and immunohistochemical staining. Autophagy was analyzed using a transmission electron microscope and western blotting. The malignancy of tumor progression in vitro and in vivo was analyzed based on cell viability, apoptosis and proliferation, migration and invasion, and xenograft model. Glial cells were marked by antibodies via immunohistochemical staining.ResultsThe human protein microarray identified 577 candidate P7C3 target proteins. The global profile of P7C3 target proteins indicated that P7C3 regulates glycolysis. Metabolic experiments confirmed that P7C3 regulates aerobic glycolysis in glioma cells. The underlying mechanism of P7C3 was found to be direct targeting PGK1 at lysine residues and asparagine residues, and the specific P7C3-PGK1 interaction led to decreased protein level and total intracellular kinase activity of PGK1. The Cancer Genome Atlas and Chinese Glioma Genome Atlas databases indicated that the mRNA level of PGK1 is significantly increased in high-grade glioma, and the abnormally high mRNA level of PGK1 is associated with a poor prognosis in patients with glioma, suggesting that PGK1 is a promising target for glioma therapy. The inhibition of PGK1 and the subsequent suppression of aerobic glycolysis caused by P7C3 inhibited the malignant growth of glioma in vitro and in vivo. Furthermore, P7C3 did not damage normal glial cells under concentration, which exhibit an inhibitory effect on gliomas.ConclusionsThis study revealed that P7C3 suppresses glioma by regulating aerobic glycolysis via directly targeting PGK1. Furthermore, we identified the P7C3 target proteins for the first time which is expected to provide scientific clues for future studies

The Bearing Capacity of Dam Body Structure with Cemented and Weathered Materials: A Constitutive Model

The Cemented Sand, Gravel, and Rock (CSGR) dam of Xijiang is the world’s first dam to use weathered material. As mechanical tests have shown significant low-strength and nonlinear characteristics in the constitutive curve of CSGR with weathered materials, a more rigorous approach is required in calculation and analysis. Based on the project, a constitutive model of CSGR with weathered material is constructed in the research. Then, the bearing capacity of the dam is studied by using the strength-reduction method and the overload method on the basis of the constitutive model. In order to further obtain the real bearing capacity of the dam, this paper also considers the hydraulic fracturing factor and analyzes its influence at the same time. Conclusions drawn are then applied to the Xijiang project, where the effect is promising

The Cemented Material Dam: A New, Environmentally Friendly Type of Dam

The first author proposed the concept of the cemented material dam (CMD) in 2009. This concept was aimed at building an environmentally friendly dam in a safer and more economical way for both the dam and the area downstream. The concept covers the cemented sand, gravel, and rock dam (CSGRD), the rockfill concrete (RFC) dam (or the cemented rockfill dam, CRD), and the cemented soil dam (CSD). This paper summarizes the concept and principles of the CMD based on studies and practices in projects around the world. It also introduces new developments in the CSGRD, CRD, and CSD

Investigation on Hydraulic Fracturing and Flexible Anti-Hydrofracturing Solution for Xiaowan Arch Dam

Understanding hydraulic fracturing in concrete super-high arch dams is vital for the implementation of safety measures on the bearing surface. In this study, we conducted tests on hydraulic fracturing for the Xiaowan arch dam (294.5 m) to analyze concrete behavior at cracks under various stress conditions. The risk of hydraulic fracturing near the dam heel was identified without compressive stress. Addressing this, we propose a flexible anti-hydrofracturing system using GB sealing material and a spray polyurea coating. Simulation tests on three schemes: ‘3 mm GB plate + 4 mm polyurea’, ‘1 mm GB glue + 5 mm polyurea’, and ‘7 mm polyurea’ showed effective prevention of hydrofracturing at concrete crack openings of 5 mm, 8 mm, or 10 mm under 300 m water pressure. Field tests supported ‘3 mm GB plate + 4 mm polyurea’ and ‘7 mm polyurea’ as optimal solutions for dam sections. Implementation involves a protective block layout with ‘3 mm GB plate + 4 mm polyurea’ on blocks and ‘7 mm polyurea’ in interval zones and corners. Since 2008, maximum leakage, including rock foundation, has remained minimal at 2.78 L/s under regular water levels. These insights aid similar concrete dams in optimizing safety systems

Biodegradation

Degradation and decolorization of monosodium glutamate wastewater (MSGW) with Coriolus versicolor were firstly carried out. The effects of various operation parameters namely wastewater concentrations, pH, culture time and incidence of sterilization on maximum percentage of degradation and decolorization of wastewater were investigated. Studies of mycelium and enzyme for C versicolor degradation and decolorization were estimated in this study. Ten percentage of wastewater concentration and pH = 5.0 were found to be the most suitable ones among the other experiments. The highest degradation and decolorization efficiency of wastewater was obtained at the fifth day of cultivation, which was displayed with more than 70% chemical oxygen demand removal, 83% total sugar removal and 55% color removal, respectively. Sterile operation had no remarkable effect on the degradation and decolorization efficiency for C. versicolor. Mycelium and the extra cellular fungal enzyme were both necessary for the degradation and decolorization of MSGW. C versicolor possesses great potential and economic advantages in MSGW treatment.Degradation and decolorization of monosodium glutamate wastewater (MSGW) with Coriolus versicolor were firstly carried out. The effects of various operation parameters namely wastewater concentrations, pH, culture time and incidence of sterilization on maximum percentage of degradation and decolorization of wastewater were investigated. Studies of mycelium and enzyme for C versicolor degradation and decolorization were estimated in this study. Ten percentage of wastewater concentration and pH = 5.0 were found to be the most suitable ones among the other experiments. The highest degradation and decolorization efficiency of wastewater was obtained at the fifth day of cultivation, which was displayed with more than 70% chemical oxygen demand removal, 83% total sugar removal and 55% color removal, respectively. Sterile operation had no remarkable effect on the degradation and decolorization efficiency for C. versicolor. Mycelium and the extra cellular fungal enzyme were both necessary for the degradation and decolorization of MSGW. C versicolor possesses great potential and economic advantages in MSGW treatment

Bioresour. Technol.

Biodegradation and decolorization of monosodium glutamate wastewater were carried out by using an acidophilus yeast strain of Saccharomyces cerevisiae and Coriolus versicolor. For the yeast treatment, the highest COD removal and reducing sugar removal efficiency were 76.6% and 80.2%, respectively. The color removal was only 2%. For C. versicolor treatment, the highest COD removal, color removal and reducing sugar removal efficiencies were 78.7%, 56.5% and 90.9%, respectively. The synergic treatment process, in which the yeast and C versicolor were successively applied,exhibited great advantage over the individual process. (c) 2006 Elsevier Ltd. All rights reserved.Biodegradation and decolorization of monosodium glutamate wastewater were carried out by using an acidophilus yeast strain of Saccharomyces cerevisiae and Coriolus versicolor. For the yeast treatment, the highest COD removal and reducing sugar removal efficiency were 76.6% and 80.2%, respectively. The color removal was only 2%. For C. versicolor treatment, the highest COD removal, color removal and reducing sugar removal efficiencies were 78.7%, 56.5% and 90.9%, respectively. The synergic treatment process, in which the yeast and C versicolor were successively applied,exhibited great advantage over the individual process. (c) 2006 Elsevier Ltd. All rights reserved

Shear Strength of Cemented Sand Gravel and Rock Materials

Shear strength is currently a significant parameter in the design of cemented sand gravel and rock (CSGR) dams. Shear strength tests were carried out to compare material without layers noumenon and layer condition. The experimental results showed good linearity in the curves of shear strength and pure grinding tests with correlation coefficients of nearly 97%. The friction coefficient was similar to that of C10 roller-compacted concrete (RCC), but the cohesion value was weaker than that of RCC. The shear strength of the CSGR layers decreased by 40% when retarding mixtures were not added and the layer was paved immediately after 4 h of waiting interval