Kaplan-Meier survival analyses of breast cancer patients.

<p>A High BRG1 expression correlates with a poorer overall survival (<i>P</i><0.001, log-rank test). B High BRG1 expression correlates with a poorer disease-specific 5-year survival (<i>P</i><0.001, log-rank test). Cum, cumulative.</p

BRG1 knockdown reduces breast cancer cells proliferative ability.

<p>A Forty-eight hours after transfection, the expression of BRG1 in MDA-MB-231 and BT-549 cells was evaluated by Western blot. Cul1 was used as a nucleoprotein loading control. B, C CCK-8 cell proliferation assay was performed after BRG1 knockdown in MDA-MB-231 and BT-549. D, E The percentage of G1 population cells was measured by flow cytometry after BRG1 knockdown in MDA-MB-231 and BT-549 cells. F Western blot analysis of the relative protein levels of cyclin D1, cyclin E and p27 in BRG1 knockdown and control group of MDA-MB-231 and BT-549 cells. β-Actin was used as a whole cell protein loading control. All experiments were carried out in triplicate. Data are shown as mean ± SE. <b>**</b><i>P</i><0.01; <b>***</b><i>P</i><0.001.</p

BRG1 staining and clinicopathological characteristics of 437 breast cancer patients.

*<p><i>P</i> values are from χ² test.</p

Knockdown of BRG1 inhibits breast cancer cell migration and invasion.

<p>A, B Cell migration assay was performed after the knockdown of BRG1 in MDA-MB-231 and BT-549 cells. C, D Matrigel cell invasion assay was performed after the knockdown of BRG1 in MDA-MB-231 and BT-549 cells. E Gelatin zymography analysis of the relative enzyme activities of MMP-2 in BRG1 knockdown and control siRNA group for both MDA-MB-231 and BT-549 cell lines. F Western blot analysis of the relative protein levels of TIMP-2 and MMP-2 in BRG1 knockdown and control siRNA group for both MDA-MB-231 and BT-549 cell lines. All experiments were carried out in triplicate. Data are shown as mean ± SE. <b>***</b><i>P</i><0.001.</p

Multivariate Cox regression analysis on 5-year overall and disease-specific survival of 437 breast cancer patients.

*<p>Coding of variables: BRG1 was coded as 1 (low expression), and 2 (high expression). Age was coded as 1 (≤50 years), and 2 (>50 years). Tumor size was coded as 1 (≤5 cm), and 2 (>5 cm). Lymph node metastasis was coded as 1 (negative), and 2 (positive). Histology grade was coded as 1 (I), and 2 (II and III). Histology type was coded as 1 (dutcal), and 2 (lobular and other).</p>†<p>CI: confidence interval.</p

Representative images of BRG1 immunohistochemical staining in human breast cancer.

<p>A, E Negative staining. B, F Weak positive staining. C, G Moderate positive staining. D, H Strong positive staining. Magnification×200 for A, B, C, and D; ×400 for E, F, G, and H.</p

Univariate Cox proportional regression analysis on 5-year overall and disease-specific survival of 437 breast cancer patients.

*<p><i>P</i> values are from Log-rank test.</p>†<p>CI: confidence interval.</p