Pulse height response of an optical particle counter to monodisperse aerosols

The pulse height response of a right angle scattering optical particle counter has been investigated using monodisperse aerosols of polystyrene latex spheres, di-octyl phthalate and methylene blue. The results confirm previous measurements for the variation of mean pulse height as a function of particle diameter and show good agreement with the relative response predicted by Mie scattering theory. Measured cumulative pulse height distributions were found to fit reasonably well to a log normal distribution with a minimum geometric standard deviation of about 1.4 for particle diameters greater than about 2 micrometers. The geometric standard deviation was found to increase significantly with decreasing particle diameter

Scalability of Optical Interconnects Based on Microring Resonators

This letter investigates the use of optical microring resonators as switching elements (SEs) in large optical interconnection fabrics. We introduce a simple physical-layer model to assess scalability in crossbar- and Benes-based architectures.We also propose a new dilated SE that improves scalability to build fabrics of several terabits per second of aggregate capacit

Optical Interconnection Architectures based on Microring Resonators

Abstract: Microring resonators are an interesting device to build integrated optical interconnects, but their asymmetric loss behavior could limit the scalability of classical optical interconnects. We present new interconnects able to increase scalability with limited complexity

Optical Interconnection Networks Based on Microring Resonators

Abstract — Interconnection networks must transport an always increasing information density and connect a rising number of processing units. Electronic technologies have been able to sustain the traffic growth rate, but are getting close to their physical limits. In this context, optical interconnection networks are becoming progressively more attractive, especially because new photonic devices can be directly integrated in CMOS technology. Indeed, interest in microring resonators as switching components is rising, but their usability in full optical interconnection architectures is still limited by their physical characteristics. Indeed, differently from classical devices used for switching, switching elements based on microring resonators exhibit asymmetric power losses depending on the output ports input signals are directed to. In this paper, we study classical interconnection architectures such as crossbar, Benes and Clos networks exploiting microring resonators as building blocks. Since classical interconnection networks lack either scalability or complexity, we propose two new architectures to improve performance of microring based interconnection networks while keeping a reasonable complexity. I

Beta Glucan: Health Benefits in Obesity and Metabolic Syndrome

Despite the lack of international agreement regarding the definition and classification of fiber, there is established evidence on the role of dietary fibers in obesity and metabolic syndrome. Beta glucan (β-glucan) is a soluble fiber readily available from oat and barley grains that has been gaining interest due to its multiple functional and bioactive properties. Its beneficial role in insulin resistance, dyslipidemia, hypertension, and obesity is being continuously documented. The fermentability of β-glucans and their ability to form highly viscous solutions in the human gut may constitute the basis of their health benefits. Consequently, the applicability of β-glucan as a food ingredient is being widely considered with the dual purposes of increasing the fiber content of food products and enhancing their health properties. Therefore, this paper explores the role of β-glucans in the prevention and treatment of characteristics of the metabolic syndrome, their underlying mechanisms of action, and their potential in food applications

Waveguiding and SERS simplified Raman spectroscopy on biological samples

Biomarkers detection at an ultra-low concentration in biofluids (blood, serum, saliva, etc.) is a key point for the early diagnosis success and the development of personalized therapies. However, it remains a challenge due to limiting factors like (i) the complexity of analyzed media, and (ii) the aspecificity detection and the poor sensitivity of the conventional methods. In addition, several applications require the integration of the primary sensors with other devices (microfluidic devices, capillaries, flasks, vials, etc.) where transducing the signal might be difficult, reducing performances and applicability. In the present work, we demonstrate a new class of optical biosensor we have developed integrating an optical waveguide (OWG) with specific plasmonic surfaces. Exploiting the plasmonic resonance, the devices give consistent results in surface enhanced Raman spectroscopy (SERS) for continuous and label-free detection of biological compounds. The OWG allows driving optical signals in the proximity of SERS surfaces (detection area) overcoming spatial constraints, in order to reach places previously optically inaccessible. A rutile prism couples the remote laser source to the OWG, while a Raman spectrometer collects the SERS far field scattering. The present biosensors were implemented by a simple fabrication process, which includes photolithography and nanofabrication. By using such devices, it was possible to detect cell metabolites like Phenylalanine (Phe), Adenosine 5-triphosphate sodium hydrate (ATP), Sodium Lactate, Human Interleukin 6 (IL6), and relate them to possible metabolic pathway variation

p53-Mediated downregulation of H ferritin promoter transcriptional efficiency via NF-Y

The tumor suppressor protein p53 triggers many of the cellular responses to DNA damage by regulating the transcription of a series of downstream target genes. p53 acts on the promoter of the target genes by interacting with the trimeric transcription factor NF-Y. H ferritin promoter activity is tightly dependent on a multiprotein complex called Bbf; on this complex NF-Y plays a major role. The aim of this work was to study the modulation of H ferritin expression levels by p53. CAT reporter assays indicate that: (i) p53 overexpression strongly downregulates the transcriptional efficiency driven by an H ferritin promoter construct containing only the NF-Y recognition sequence and that the phenomenon is reverted by p53 siRNA; (ii) the p53 C-terminal region is sufficient to elicitate this regulation and that a correct C-terminal acetylation is also required. The H ferritin promoter displays no p53-binding sites; chromatin immunoprecipitation assays indicate that p53 is recruited on this promoter by NF-Y. The p53–NF-Y interaction does not alter the NF-Y DNA-binding ability as indicated by electrophoretic mobility shift assay (EMSA) analysis. These results demonstrate that the gene coding for the H ferritin protein belongs to the family of p53-regulated genes, therefore adding a new level of complexity to the regulation of the H ferritin transcription and delineate a role for this protein in a series of cellular events triggered by p53 activation

Microfluidic platforms for cell cultures and investigations

This review covers several aspects of microfluidic devices used for culturing and monitoring of both adherent and non-adherent cells, including a multitude of applications. A comparison of available platforms with high throughput analysis, automation capability, interface to sensors and integration, is reported. Aspects, such as operational versatility of the devices, are scrutinized in terms of their analytical efficacy. It is found that due to multi-functionality capability of modern microfluidics, there is big amount of experimental data obtainable from a single device, allowing complex experimental control and efficient data correlation, particularly important when biomedical studies are considered. Hence several examples on cell culture and monitoring are given in this review, including details on design of microfluidic devices with their distinctive technological peculiarities

Cytoplasmic cleavage of IMPA1 3' UTR is necessary for maintaining axon integrity

The 3′ untranslated regions (3′ UTRs) of messenger RNAs (mRNAs) are non-coding sequences involved in many aspects of mRNA metabolism, including intracellular localization and translation. Incorrect processing and delivery of mRNA cause severe developmental defects and have been implicated in many neurological disorders. Here, we use deep sequencing to show that in sympathetic neuron axons, the 3′ UTRs of many transcripts undergo cleavage, generating isoforms that express the coding sequence with a short 3′ UTR and stable 3′ UTR-derived fragments of unknown function. Cleavage of the long 3′ UTR of Inositol Monophosphatase 1 (IMPA1) mediated by a protein complex containing the endonuclease argonaute 2 (Ago2) generates a translatable isoform that is necessary for maintaining the integrity of sympathetic neuron axons. Thus, our study provides a mechanism of mRNA metabolism that simultaneously regulates local protein synthesis and generates an additional class of 3′ UTR-derived RNAs

Proteomic analysis of S-nitrosylated nuclear proteins in rat cortical neurons

The ability of neurons to modulate gene expression in response to extrinsic signals is necessary for proper brain function. S-nitrosylation is the covalent attachment of a nitric oxide (NO) moiety to cysteine thiols and is critical for transducing extracellular stimuli into specific patterns of gene expression. In the cerebral cortex, S-nitrosylation of histone deacetylase 2 (HDAC2) is required for gene transcription during neuronal development, however only few nuclear targets of Snitrosylation have been identified to date. Here, we used S-nitrosothiol Resin Assisted Capture (SNORAC) coupled with mass spectrometry analysis to identify 614 S-nitrosylated nuclear proteins. Of these, 131 proteins had never been shown to be S-nitrosylated in any system, and 612 are new targets of S-nitrosylation in neurons. The site(s) of S-nitrosylation were identified for 59% of the targets, and motifs containing single lysines found at 33% of these sites. In addition, lysine motifs were found to be necessary for promoting S-nitrosylation of HDAC2 and Methyl-CpG Binding Protein 3 (MBD3). Moreover, S-nitrosylation of the histone binding protein RBBP7 was found to be necessary for dendritogenesis. Overall, our study provides the first extensive characterization of Snitrosylated nuclear proteins in neurons and identifies putative S-nitrosylation motifs that may be shared with other targets of nitric oxide signaling