14 research outputs found

    Fatty acid composition of total lipids in A) seed oil, B) pulp oil and C) whole berry oil of four cultivars RC-4, E6590, FR-14 and Harvest Moon.

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    <p>Results represent the mean ± SD of three biological replicates. Minor fatty acids (accounting in total for <3% of the fatty acid composition) are not shown. 16∶0, palmitic acid; 16∶1, palmitoleic acid; 18∶0, stearic acid; 18∶1c9, oleic acid; 18∶1c11, <i>cis</i>-vaccenic acid; 18∶2, linoleic acid; 18∶3, α-linolenic acid.</p

    Sea buckthorn sequences associated with isoprenoid biosynthesis by A) cytosolic MVA pathway and B) plastidial MEP pathway.

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    <p>Enzymes for which sea buckthorn sequences have been identified are shown in green. Abbreviations used for intermediates are: 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA); mevalonic acid (MVA); mevalonate-5-phosphate (MVAP); MVAPP mevalonate-5-diphosphate (MVAPP); Isopentenyl diphosphate (IPP); Dimethylallyl-diphosphate (DMAPP); d-glyceraldehyde-3-phosphate (GAP); 1-deoxy-D-xylulose-5-phosphate (DXP); 2- C-methyl-D- erythritol-4- phosphate (MEP); 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (CDP-ME); 2-phospho-4-(cytidine 5'C-diphospho) 2-C-methyl-D-erythritol (CDP-ME2P); 2-C- methyl-D- erythritol 2,4-cyclodiphosphate (MECDP); 1-hydroxy-3-methyl-2-(E)-butenyl-4-diphosphate (HMBPP); Abbreviations used for enzymes are: Acetoacetyl-CoA thiolase (AACT); 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS); HMG-CoA reductase (HMGR); mevalonate kinase (MVK); diphosphomevalonate kinase (PMK); diphosphomevalonate decarboxylase (PMD); isopentenyl diphosphate isomerase (IPP/IPI); 1-deoxy-d-xylulose-5-phosphate synthase (DXS); 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR); 4-diphosphocytidyl-2-C-methyl-d-erythritol synthase (ISPD/CMS); 4-(cytidine 5′-diphospho-2-C-methyl-d-erythritol kinase (ISPE/CMK); 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase (ISPF/MCS); 4-hydroxy-3-methyl but-2-enyl diphosphate synthase (ISPG/HDS); 4-hydroxy-3-methyl but-2-enyl diphosphate reductase (ISPH/HDR).</p

    Sea buckthorn sequences associated with fatty acid A) and triacylglycerol B) biosynthetic pathways.

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    <p>Enzymes with names in full are represented on the left, and with names in short are represented on the right. Enzymes known to function at specific steps of the pathway are represented in black. Enzymes for which sea buckthorn sequences have been identified are shown in green. EAR, enoyl-acyl-ACP-reductase; HAD, 3-hydroxyacyl-ACP dehydratase. Enzymes responsible for phosphatidylcholine acyl editing in TAG assembly (LPCAT, CPT/PDCT, PDAT) are shown in the shaded box in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034099#pone-0034099-g006" target="_blank">figure 6B</a>.</p

    RT-PCR analysis of genes involved in fatty acid and triacylglycerol biosynthesis in seed and pulp tissues at different developmental stages of fruit from RC-4 cultivar.

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    <p>G, G/Y, Y/O and O/R represent fruits harvested at green (6 August), green/yellow (17 August), yellow/orange (31 August) and orange/red (22 October) stages, respectively. <i>Ubiquitin5</i> (<i>UBQ5)</i> was used as loading control.</p
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