8 research outputs found
Comparison of endometrial mRNA levels for selected genes in the endometrium determined by microarray analysis and real-time semi-quantitative PCR (qPCR)<sup>a</sup>.
<p>Comparison of endometrial mRNA levels for selected genes in the endometrium determined by microarray analysis and real-time semi-quantitative PCR (qPCR)<sup><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069444#nt102" target="_blank">a</a></sup>.</p
Experimental design and results for selection of beef heifers for uterine capacity for early pregnancy using a serial timed artificial insemination (AI) approach.
<p>See text for detailed description of results.</p
Heatmap of pairwise correlations and principal component analysis (PCA) of microarray data.
<p>(A) Microarray data were filtered for detectable probes and normalized with the BioConductor package vsn. Normalized data were used for calculation of pairwise distances and drawing of a heatmap by use of the BioConductor package geneplotter. Each column represents one sample and shows the correlation to all samples (including itself), with red for correlation  = 1 and blue for the lowest observed correlation. Note the clear homogeneity in the samples from fertility classified heifers (HF, high fertile; SF, subfertile; IF, infertile). (B) PCA is a plot distribution indicating the source of greatest variation in the overall transcriptional profiles of the samples. Each symbol represents one replicate. Note the clear lack of separation of samples based on fertility classifications (HF, high fertile; SF, subfertile; IF, infertile).</p
Genome-wide association or Manhattan plot of significance values for high fertile compared to low fertile heifers.
<p>The results of the genome-wide association analysis are shown for chromosomes 1 through 29 and the X chromosome (labeled as 30). The results are plotted by the −log<sub>10</sub> significance values on the y-axis and the chromosomal location for each SNP tested on the x-axis. The red line represents the Wellcome Trust threshold for moderate evidence for significance association.</p
Selected overrepresented functional categories for differentially expressed genes in High Fertile as compared to Infertile heifers (nominal P-value <0.01).
<p>Selected overrepresented functional categories for differentially expressed genes in High Fertile as compared to Infertile heifers (nominal P-value <0.01).</p
Circulating concentrations of progesterone in a subset of fertility classified heifers after ovulation.
<p>Note the lack of difference in serum progesterone levels in high fertile (HF, n = 13), subfertile (SF, n = 12) and infertile (IF, n = 10) heifers.</p
Venn diagram showing the number of unique or common transcripts between the endometrium of fertility-classified heifers (HF, high fertile; SF, subfertile; IF, infertile).
<p>Up-regulated (red) and down-regulated (black) genes are presented (P<0.01 and no false discovery rate with greater than 1.5-fold change). A few up- and down-regulated genes are highlighted in the boxes.</p