Taxonomy and phylogeny of the genus Mycosphaerella and its anamorphs

Historically plant pathogenic species of Mycosphaerella have been regarded as host-specific, though this hypothesys has proven difficult to test largely due to the inavailability of fungal cultures. During the course of the past 20 years a concerted effort has been made to collect these fungi, and devise methods to cultivate them. Based on subsequent DNA sequence analyses the majority of these species were revealed to be host-specific, though some were not, suggesting that no general rule can be applied. Furthermore, analysis of recent molecular data revealed Mycosphaerella to be poly- and paraphyletic. Teleomorph morphology was shown to be too narrowly defined in some cases, and again too widely in others. Mycosphaerella and Teratosphaeria as presently circumscribed represent numerous different genera, many of which can be recognised based on the morphology of their 30 odd associated anamorph genera. Although Mycosphaerella is generally accepted to represent one of the largest genera of ascomycetous fungi, these data suggest that this is incorrect, and that Mycosphaerella should be restricted to taxa linked to Ramularia anamorphs. Furthermore, other anamorph form genera with Mycosphaerella-like teleomorphs appear to represent genera in their own right

Niche sharing reflects a poorly understood biodiversity phenomenon

Eucalyptus spp. are susceptible to a large number of foliar pathogens, some of which can cause serious defoliation and die-back. In this study, a single leaf spot on a Eucalyptus leaf collected in Madagascar revealed an unusual association of microfungi with disease symptoms. Initial observations indicated that the leaf spot was associated with Mycosphaerella marksii, a common pathogen of eucalypts. However, more intensive scrutiny showed the presence of several other microfungi co-occurring in this, and other leaf spots on the leaf. A total of 41 single conidial propagules were subsequently obtained from a single lesion for morphological study and DNA sequence comparisons. Based on these data, 11 members of the Capnodiales, including one species of Pestalotiopsis (Xylariales), were observed. Of the capnodialean taxa, nine could be cultivated, which revealed one known species, M. marksii, two taxa in the Cladosporium cladosporioides species complex that were not treated here, and six new species, including Passalora intermedia, Pseudocercospora madagascariensis, Teratosphaeria hortaea, Toxicocladosporium chlamydosporum, T. rubrigenum and T. veloxum. Results of this study highlight a remarkable fungal biodiversity that can occur within a very specific niche. Furthermore, the results emphasise the importance of verifying the identity of fungal isolates in culture, as many taxa, especially those of the Capnodiales, frequently co-occur in the same niche, lesion or leaf spo

The enigma of Calonectria species occurring on leaves of Ilex aquifolium in Europe

Species of Calonectria are common saprobes and plant pathogens on a wide range of hosts occurring in subtropical to tropical regions of the world. The aim of the present study was to resolve the status of new Calonectria collections obtained on Ilex leaves from France. Based on DNA sequence data of their b-tubulin and histone gene regions, as well as morphology, the new collections matched the ex-type strain of Cylindrocladium ilicicola. On the host and in culture, yellow to brownish-yellow perithecia were observed that did not strain red in 3 % KOH. Based on these results, C. ilicicola and its purported teleomorph, Ca. pyrochroa, were shown to represent two distinct species, as the latter has bright red perithecia that strain purple in KOH. A new combination, Ca. lauri, based on Tetracytum lauri, is subsequently proposed for C. ilicicola. Calonectria lauri is distinct from Ca. ilicicola, a pathogen commonly associated with Cylindrocladium black rot of peanut. Finally, Ca. canadiana is proposed asnew name for Cy. canadiense, which is a nursery pathogen involved with root rot of several tree genera in Quebec, Canada

Multigene phylogeny and mating tests reveal three cryptic species related to Calonectria pauciramosa

Calonectria pauciramosa is a pathogen of numerous plant hosts worldwide. Recent studies have indicated that it included cryptic species, some of which are identified in this study. Isolates from various geographical origins were collected and compared based on morphology, DNA sequence data of the ß-tubulin, histone H3 and translation elongation factor-1 regions and mating compatibility. Comparisons of the DNA sequence data and mating compatibility revealed three new species. These included Ca. colombiana sp. nov. from Colombia, Ca. polizzii sp. nov. from Italy and Ca. zuluensis sp. nov. from South Africa, all of which had distinguishing morphological features. Based on DNA sequence data, Ca. brasiliensis is also elevated to species leve

Colletotrichum species with curved conidia from herbaceous hosts

Colletotrichum (Glomerellaceae, Sordariomycetes) species with dark setae and curved conidia are known as anthracnose pathogens of a number of economically important hosts and are often identified as C. dematium. Colletotrichum dematium has been synonymised with many species, including the type of the genus, C. lineola. Since there is no living strain of the original material of either species available, we re-collected C. lineola from the original location to serve as an epitype of that name, and chose an appropriate epitype specimen and associated strain of C. dematium from the CBS collection. A multilocus molecular phylogenetic analysis (ITS, ACT, Tub2, CHS-1, GAPDH, HIS3) of 97 isolates of C. lineola, C. dematium and other Colletotrichum species with curved conidia from herbaceous hosts resulted in 20 clades, with 12 clades containing strains that had previously been identified as C. dematium. The epitype strains of C. lineola and C. dematium reside in two closely related clades. Other clades represent four previously undescribed species, C. anthrisci, C. liriopes, C. rusci and C. verruculosum, isolated respectively from Anthriscus in the Netherlands, Liriope in Mexico, Ruscus in Italy and Crotalaria in Zimbabwe. The new combinations C. spaethianum and C. tofieldiae are made. Colletotrichum truncatum is epitypified, as well as C. circinans, C. curcumae and C. fructi. Three further unidentified Colletotrichum taxa were detected in the phylogenetic analysis, which may require description after further research. Each species is comprehensively described and illustrate

Mycosphaerella podagrariae - a necrotrophic phytopathogen forming a special cellular interaction with its host Aegopodium podagraria

We present a new kind of cellular interaction found between Mycosphaerella podagrariae and Aegopodium podagraria, which is remarkably different to the interaction type of the obligate biotrophic fungus Cymadothea trifolii, another member of the Mycosphaerellaceae (Capnodiales, Dothideomycetes, Ascomycota) which we have described earlier. Observations are based on both conventional and cryofixed material and show that some features of this particular interaction are better discernable after chemical fixation. We were also able to generate sequences for nuclear ribosomal DNA (complete SSU, 5.8 S and flanking ITS-regions, D1–D3 region of the LSU) confirming the position of M. podagrariae within Mycosphaerellacea

Fungal phoenix rising from the ashes?

During May 2010, sporocarps of what appeared to be an Armillaria sp. were found in large clumps in historic Kirstenbosch Botanical Gardens on the foot of Table Mountain, Cape Town, South Africa. These sporocarps could be physically linked to the roots of unidentified dead trees and Protea spp. The aim of this study was to identify the Armillaria sp. found fruiting in Kirstenbosch. To achieve this goal isolates were made from the mycelium under the bark of dead roots linked to sporocarps. The ITS and IGS-1 regions were sequenced and compared to sequences of Armillaria spp. available on GenBank. Cladograms were generated using ITS sequences to determine the phylogenetic relationship of the isolates with other Armillaria spp. Sequence comparisons and phylogenetic analyses showed that the isolates represented A. mellea. They were also identical to isolates of this species previously discovered in the Company Gardens in South Africa and introduced from Europe apparently by the early Dutch Settlers. Armillaria mellea is alien and apparently invasive in Cape Town, fruits profusely and has the potential to spread to sensitive native forests on the foothills of the City

Calonectria species associated with cutting rot of Eucalyptus

Decline in the productivity of Eucalyptus hybrid cutting production in the Guangdong Province of China is linked to cutting rot associated with several Calonectria spp. The aim of this study was to identify these fungi using morphological and DNA sequence comparisons. Two previously undescribed Calonectria spp., Ca. pseudoreteaudii sp. nov. and Ca. cerciana sp. nov. were identified together with Ca. pauciramosa. Calonectria pseudoreteaudii resides in the Ca. reteaudii complex and Ca. cerciana is closely related to Ca. morganii. Connected to the discovery of Ca. pseudoreteaudii, species in the Ca. reteaudii complex were re-considered and the group is shown to accommodate two cryptic species. These originate from Australia and are described as Ca. queenslandica sp. nov. and Ca. terrae-reginae sp. nov