Additional file 1: Figure S1. of Che-1 sustains hypoxic response of colorectal cancer cells by affecting Hif-1Îą stabilization

Che-1 is involved in the metabolic switch in response to hypoxia. A- and B- HT29 (A) and A549 (B) cells were transiently transfected with stealth siRNA negative control (siControl) or siRNA Che-1 (siChe-1) and exposed to hypoxia for 16 h where indicated and pH was measured. C- and D- Score plots indicating metabolic differences between hypoxic and normoxic samples from HT29 (C) and A549 (D) cells transiently transfected as in A. E- HT29 cells were transfected as in A and the medium lactate content was evaluated. (TIF 5841 kb

Additional file 2: Figure S2. of Che-1 sustains hypoxic response of colorectal cancer cells by affecting Hif-1α stabilization

Che-1 regulates genes transcription in response to hypoxia. A- Quantitative RT–PCR (qRT–PCR) for metabolic genes expression was performed in HT29 cells transiently transfected with Stealth siRNA negative control (siControl) or siRNA Che-1 (siChe-1) and exposed to hypoxia for 4 h. Values were normalized to RPL19 mRNA expression. Error bars represent the standard error of three different experiments. *P = 0,0010, **P ≤ 0,0003, ***P ≤ 0,004, n.s., not significant. (TIF 1835 kb