2 research outputs found
Presencia de secuencias ERIC en Chlamydia trachomatis
Introduction. One of the methods used for the
typifi cation and for bacterial genetic diversity study
is ERIC-PCR. The presence of enterobacterial
repetitive intergenic consensus sequences (ERIC)
has been described in most Gram negative bacteria,
but their presence in bacteria, such as Chlamydia
trachomatis, has not been investigated.
Objective. To search in silico and in vitro for ERIC
sequences in the Chlamydia trachomatis genome.
Materials and Methods. In silico, ERIC
sequences were searched with the bioinformatics
tool called FASTA, downloading the genomes of
Chlamydia trachomatis D/uw-3/cx, Chlamydia
trachomatis 434/Bu, Chlamydia trachomatis L2b/
UCH-1/proctitis, Chlamydia trachomatis A/har-
13, Chlamydia muridarum Nigg, Chlamydophila
pneumoniae TW-183, Aeromonas hydrophila, and Escherichia coli K12 deposited in the NCBI
database. in vitro, the ERIC-PCR technique
was standardized with strains of Chlamydia
trachomatis serovars D, L2, and L3.
Results. Were obtained the number of alignments,
the alignment region of each ERIC primer, and
the expectation (E) and score (S) values of each
genome. Afterwards, the presence of ERIC
sequences in strains of Chamydia trachomatis
serovars D, L2, and L3 was determined, being 44
°C the optimal alignment temperature in the PCR.
Conclusions. With the obtained results we can
suggest that the ERIC sequences are present in
the Chlamydia trachomatis genome, if this fi nding
is reproduced in all the serovars, a new typing
method for this bacterium could establish.Introducción. Uno de los métodos empleados
para la tipifi cación y el estudio de la diversidad
genética bacteriana es la ERIC-PCR. La presencia
de las secuencias consenso intergénicas repetitivas
enterobacterianas (ERIC) se ha descrito en la
mayorÃa de las bacterias Gram negativas, pero no
se ha investigado su presencia en bacterias como
Chlamydia trachomatis.
Objetivo. Realizar la búsqueda in silico e in vitro
de las secuencias ERIC en el genoma de Chlamydia
trachomatis.
Materiales y Métodos. En el estudio in silico,
las secuencias ERIC se buscaron mediante la
herramienta bioinformática FASTA y empleando
los genomas de C. trachomatis D/uw-3/cx, C.
trachomatis 434/Bu, C. trachomatis L2b/UCH-1/
proctitis, C. trachomatis A/har-13, C. muridarum
Nigg, C. pneumoniae Tw-183, Aeromonas hydrophila
y Escherichia coli K12, depositadas en la
base de datos del NCBI. In vitro, se estandarizó
la PCR empleando cepas de C. trachomatis serovariedades
D, L2 y L3.
Resultados. Se obtuvieron el número de alineamientos,
la región de alineamiento de cada iniciador
ERIC y los valores de expectación (E) y score
(S) de cada genoma estudiado. Posteriormente, se
determinó la presencia de la secuencias ERIC en
las cepas de C. trachomatis serovariedades D,
L2 y L3, siendo 44ºC la temperatura óptima de
alineamiento en la PCR. Conclusiones. Con los resultados obtenidos, podemos
sugerir que en el genoma de C. trachomatis
existen las secuencias ERIC; si este hallazgo se
reproduce en todas las serovariedades, se podrÃa
establecer un nuevo método de tipifi cación para
esta bacteria
Correct Identification of Ochrobactrum anthropi From Blood Culture Using 16rRNA Sequencing: A First Case Report in an Immunocompromised Patient in Mexico
The present report describes the misidentification of Brucella spp. from a positive blood culture using traditional microbiology tests. A molecular test identified the bacterium as Ochrobactrum anthropi. According to the information available, this report is the first to include this type of case in Mexico