Brain 5-HT.

<p>Brain 5-HT content in ng/mg tissue in (a) prefrontal cortex (b) frontal cortex and (c) hippocampus. Animals were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035916#pone-0035916-g002" target="_blank">Figure 2</a>. Only vehicle treated animals are shown. N = 5–6/group. * different from Water, +different from TRP+, ♦ different from corresponding group in BALBc mice. 5-HT content was lower at baseline in BALBc in the hippocampus; TRP− significantly decreased 5-HT content in the hippocampus; TRP+ did not consistently increase 5-HT content; TRP− was consistently lower than TRP+ in all regions.</p

Time course of ATD Moja-De in both strains.

<p>(a) Plasma = plasma tryptophan (µg/ml plasma) (b) HPC TRP = tryptophan in hippocampus (µg/mg tissue) (c) HPC-5-HIAA = 5-HIAA in hippocampus (ng/mg tissue). N = 3–4/group for C57, N = 4–8/group for BALBc. * different from Time 0 and ♦ different from corresponding group in BALBc mice.</p

Brain tryptophan.

<p>Brain tryptophan content in µg/mg tissue (a) prefrontal cortex (b) frontal cortex and (c) hippocampus. BALBc and C57 mice received two treatments by gavage of TRP+, TRP− mixtures or water vehicle at 30 minute intervals followed by saline or NSD 1015 to inhibit amion acid decarboxylase at 2 hours. Animals were killed 2.5 hours after the first treatment. Data are collapsed for NSD treatment as no significant effects were predicted or observed. N = 11–12/group. * different from Water. +different from TRP+, ♦ different from corresponding group in BALBc mice. TRP+ and TRP− effectively raised and lowered TRP respectively, although the depletion condition was more consistently effective than the control condition. Similar effects were detected in both strains.</p

Brain 5-HTP.

<p>Brain 5-HTP content in ng/mg tissue in (a) prefrontal cortex (b) frontal cortex and (c) hippocampus. Animals were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035916#pone-0035916-g002" target="_blank">Figure 2</a>. Only NSD 1015-treated animals are shown. N = 6/group. * different from Water, +different from TRP+, ♦ different from corresponding group in BALBc mice. TRP− decreased 5-HT synthesis in all brain regions, but the results were only statistically relevant in C57 mice; TRP+ did not increase 5-HT synthesis; at baseline, BALBc mice had a lower level of 5-HT synthesis in the hippocampus and the prefrontal cortex, but not in the frontal cortex.</p

Time Course of Behavioral Experiment.

<p>Time Course of Behavioral Experiment.</p

Time Course of Biochemistry Experiment.

<p>Time Course of Biochemistry Experiment.</p

Behavioral data showing time spent in the light.

<p>Only TRP+- and TRP− -treated animals are shown. N = 14/group. +different from TRP+, ♦ different from corresponding group in BALBc mice.</p