<i>Trypanosoma cruzi</i> diagnostic Real-Time PCR results of samples analysed according to the protocols used in the study based on the combination of different sample treatments, DNA extraction methods and Real-Time PCR procedures.

<p><i>Trypanosoma cruzi</i> diagnostic Real-Time PCR results of samples analysed according to the protocols used in the study based on the combination of different sample treatments, DNA extraction methods and Real-Time PCR procedures.</p

<i>Trypanosoma cruzi</i> diagnostic Real-Time PCR results obtained in the eight protocols assessed for the 22 discordant samples.

<p><i>Trypanosoma cruzi</i> diagnostic Real-Time PCR results obtained in the eight protocols assessed for the 22 discordant samples.</p

Degree of agreement between protocols A and H based on a Bland-Altman plot of the nine samples with quantifiable results in both methods.

<p>Each sample is represented by plotting the mean of the measurements obtained in protocols A and H on the x-axis and the difference of the same two values on the y-axis. SD: standard deviation. Log₁₀ par. eq./10 mL: logarithmic values of parasite equivalents in 10 mL of blood.</p

Cohen’s kappa coefficient (K) results for the comparison between protocols with the sample treatment as the only variable.

<p>K values are shown including and excluding inhibited samples.</p